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Schistosoma mansoni: a comparative study of schistosomula transformed mechanically and by skin penetration. Electrophysiological responses to a wide range of substances

Published online by Cambridge University Press:  06 April 2009

D. P. Thompson ,
R. A. Pax  and
J. L. Bennett
D. P. Thompson
Affiliation:
Departments of Pharmacology and Toxicology, and Zoology, and Neuroscience Program, Michigan State University, East Lansing, Michigan 48824
R. A. Pax
Affiliation:
Departments of Pharmacology and Toxicology, and Zoology, and Neuroscience Program, Michigan State University, East Lansing, Michigan 48824
J. L. Bennett
Affiliation:
Departments of Pharmacology and Toxicology, and Zoology, and Neuroscience Program, Michigan State University, East Lansing, Michigan 48824
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Summary

Volume conducted potentials were recorded from schistosomula of Schistosoma mansoni transformed mechanically (MS) and by skin penetration (SS). The spontaneous electrical activity recorded consisted of bi and triphasic transients ranging from 20 to 200 μV in amplitude and 10 to 300 msec in duration. Low amplitude potentials occurred at a much greater frequency than large amplitude potentials, which appeared to correlate with peristaltic-like contractions of the schistosomulum's musculature. Electrical activity in the schistosomulum was highly sensitive to a number of agents believed to affect metabolic pathways, the neuromuscular system or the host/parasite interface of adult schistosomes. Among the most reactive substances were potassium antimony tartrate, eserine, poly-L-arginine and potassium cyanide. Over a wide range of experimental treatments, electrophysiological responses in schistosomula transformed from cercariae by mechanical decaudation and skin penetration were remarkably similar, supporting the notion that MS are suitable material for in vitro immunochemical, biochemical and physiological study. Some treatments, however, were more or less effective in altering electrophysiological activity and motility in the schistosomulum than in adult S. mansoni. This suggests that significant physiological alterations may occur during development from skin stage to adult parasites concomitant with immunochemical and morphological changes already known to occur.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 3 , June 1984 , pp. 477 - 489
Copyright
Copyright © Cambridge University Press 1984

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