Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-26T01:15:13.245Z Has data issue: false hasContentIssue false
  • English
  • Français

Scanning electron microscopy of the duodenal mucosa of lambs infected with Trichostrongylus colubriformis

Published online by Cambridge University Press:  06 April 2009

I. K. Barker
I. K. Barker
Affiliation:
Department of Veterinary Paraclinical Sciences, University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria 3030, Australia
Get access Rights & Permissions [Opens in a new window]

Extract

Duodenal mucosae of uninfected lambs and lambs inoculated at least 16 days earlier with 85000–140000 Trichostrongylus colubriformis larvae were examined with the scanning electron microscope. Normal duodenum had tall spatulate villi with surface folds upon which goblet cells and a regular pattern of hexagonal enterocytes were seen. Micro villi on normal enterocytes were closely packed and imparted a granular surface texture. In heavily infected areas of gut the villi were atrophic, the mucosa sometimes being composed of irregular masses and ridges, with crypt mouths, often surrounded by collars of cells, opening into the surface. More severely affected mucosae were flat, with protuberant collars of cells surrounding crypt mouths. There were rounded bodies, interpreted as sloughing enterocytes, or inflammatory cells, on the mucosal surface. Apices of enterocytes were domed and microvilli were sparse and irregular. Micro-organisms were numerous on cell surfaces. Nematodes were located in sinuous thin-walled tunnels in the epithelium. The mucosal microtopography is compared with that of coeliac disease of humans, nippostrongylosis in rats and with villus atrophy in pigs.

Type
Research Article
Information
Parasitology , Volume 67 , Issue 3 , December 1973 , pp. 307 - 314
Copyright
Copyright © Cambridge University Press 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Asquith, P., Johnson, A. G. & Cooke, W. T. (1970). Scanning electron microscopy of normal and celiac jejunal mucosa. American Journal of Digestive Diseases 15, 511–21.CrossRefGoogle ScholarPubMed
Balcerzak, S. P., Lane, W. C. & Bullard, J. W. (1970). Surface structure of intestinal epithelium. Gastroenterology 58, 49–55.CrossRefGoogle ScholarPubMed
Barker, I. K. (1973). A study of the pathogenesis of Trichostrongylus colubriformis infection in lambs with observations on the contribution of gastrointestinal plasma loss. International Journal for Parasitology 3 (accepted for publication).CrossRefGoogle ScholarPubMed
Croft, D. N., Loehry, C. A. & Creamer, B. (1968). Small bowel cell-loss and weight loss in the coeliac syndrome. Lancet 2, 68–70.CrossRefGoogle Scholar
Demling, L., Becker, V. & Classen, M. (1969). Examination of the mucosa of the small intestine with the scanning electron microscope. Digestion 2, 51–60.CrossRefGoogle ScholarPubMed
Fitzsimmons, W. M. (1969). Pathogenesis of the trichostrongyles. Helminthological Abstracts 38, 139–90.Google Scholar
Grey, R. D. (1972). Morphogenesis of intestinal villi. I. Scanning electron microscopy of the duodenal epithelium of the developing chick embryo. Journal of Morphology 137, 193–213.CrossRefGoogle ScholarPubMed
Jenkins, T. (1970). A morphological and histochemical study of Trichuris suis (Schrank, 1788) with special reference to the host-parasite relationship. Parasitology 61, 357–74.CrossRefGoogle Scholar
Loehry, C. A. & Creamer, B. (1969). Three-dimensional structure of the rat small intestinal mucosa related to mucosal dynamics. Gut 10, 112–20.CrossRefGoogle ScholarPubMed
Marsh, M. N., Brown, A. C., & Swift, J. A., (1970). The surface ultrastructure of the small intestinal mucosa of normal control human subjects and of patients with untreated and treated coeliac disease using the scanning electron microscope. In Coeliac Disease: Proceedings of a Symposium held at the Royal Postgraduate, Medical School London, 1969 (ed. Booth, C. C., and Dowling, R. H.), pp. 26–44. London: Churchill Livingstone.Google Scholar
Marsh, M. N. & Swift, J. A. (1969). A study of the small intestinal mucosa using the scanning electron microscope. Gut 10, 940–9.CrossRefGoogle ScholarPubMed
Marsh, M. N., Swift, J. A. & Williams, E. D. (1968). Studies of the small intestinal mucosa with the scanning electron micrsocope. British Medical Journal 4, 95–6.CrossRefGoogle Scholar
Mouwen, J. M. V. M. (1971). White scours in piglets. II. Scanning electron microscopy of the mucosa of the small intestine. Veterinary Pathology 8, 401–13.CrossRefGoogle Scholar
Pfefferkorn, G. E., (1970). Specimen preparation techniques. In Proceedings of the Third Annual Scanning Electron Microscopy Symposium, p. 89. Chicago: I. I. T. Research Institute.Google Scholar
Pout, D. D. (1970). The mucosal surface patterns of the small intestine of grazing lambs. British Veterinary Journal 126, 357–63.CrossRefGoogle Scholar
Swift, J. A. & Marsh, M. N. (1968). Scanning electron microscopy of rat intestinal microvilli. Lancet 2, 915.CrossRefGoogle ScholarPubMed
Symons, L. E. A. (1965). Kinetics of the epithelial cells, and morphology of villi and crypts in the jejunum of the rat infected by the nematode Nippostrongylus brasiliensis. Gastroenterology 49, 158–68.CrossRefGoogle ScholarPubMed
Symons, L. E. A. (1969). Pathology of gastrointestinal helminthiases. International Review of Tropical Medicine 3, 49–100.Google ScholarPubMed
Symons, L. E. A. & Fairbairn, D. (1962). Pathology, absorption, transport, and activity of digestive enzymes in rat jejunum parasitized by the nematode Nippostrongylus brasiliensis. Federation Proceedings 21, 913–18.Google ScholarPubMed
Symons, L. E. A. & Jones, W. O. (1970). Nematospiroides dubius, Nippostrongylus brasiliensis and Trichostrongylus colubriformis. Protein digestion in infected mammals. Experimental Parasitology 27, 496–506.CrossRefGoogle ScholarPubMed
Toner, P. G. & Carr, K. E. (1969). The use of scanning electron microscopy in the study of the intestinal villi. Journal of Pathology 97, 611–7.CrossRefGoogle Scholar
Toner, P. G., Carr, K. E., Ferguson, A. & MacKay, C. (1970). Scanning and transmission electron microscopic studies of human intestinal mucosa. Gut 11, 471–81.CrossRefGoogle ScholarPubMed
Toner, P. G., Carr, K. E., & Wyburn, G. M., (1971). The Digestive System. An Ultrastructural Atlas and Review. London: Butterworths.Google Scholar
Triet, J. S. & Browning, T. H. (1970). Epithelial cell renewal in cultured duodenal biopsies in celiac disease. New England Journal of Medicine 283, 1245–50.Google Scholar
Wattebs, W. B. & Buck, R. C. (1971). An improved simple method of specimen preparation for replicas or scanning electron microscopy. Journal of Microscopy 94, 185–7.CrossRefGoogle Scholar
Waxler, G. L. (1972). Lesions of transmissible gastroenteritis in the pig as determined by scanning electron microscopy. American Journal of Veterinary Research 33, 1323–8.Google ScholarPubMed
Wright, N. A., Watson, A. J., Morley, A. R., Appleton, D. R. & Marks, J. M. (1972). Cell production rate in mucosa of untreated coeliac disease. Gut 13, 846.Google ScholarPubMed