Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-23T14:07:43.443Z Has data issue: false hasContentIssue false
  • English
  • Français

The route of migration of Eimeria stiedae (Lindemann, 1865) sporozoites between the duodenum and bile ducts of the rabbit

Published online by Cambridge University Press:  06 April 2009

R. J. Horton
R. J. Horton
Affiliation:
Department of Pathology, University of Cambridge*
Get access Rights & Permissions [Opens in a new window]

Extract

Young rabbits were infected by mouth with sporulated Eimeria stiedae oocysts and killed at 1, 3, 5, 8, 12, 16, 24, 30, 48, 54, 72, 84, and 96 h after infection. Sporozoites were found in the lamina propria of the duodenum between 5 and 8 h after infection. Sporozoites were present in the mesenteric lymph nodes, both free and within lymphatic monocytes at between 12 and 84 h after infection. No sporozoites were observed in duodenal portal blood. These observations provide morphological evidence compatible with a lymphatic route of migration between duodenum and mesenteric lymph nodes, probably within lymphatic monocytes. The route to the bile duct epithelium is discussed and a subsequent migration in the portal blood suggested.

I wish to express my gratitude to Dr D. F. Kelly of the School of Veterinary Medicine, Cambridge, for his guidance and constructive criticism, and to Dr Elaine Rose of the Houghton Poultry Research Station, St Ives, for her invaluable advice on technical problems and for donating the oocysts for the study. I should also like to thank Dr E. A. Pierce of the Department of Animal Physiology, Babraham, and Mr P. Long of Houghton for their advice.

This study was carried out as a Research Project during the Natural Sciences Tripos, Part II Pathology course 1965, in the Department of Pathology, Cambridge.

Type
Research Article
Information
Parasitology , Volume 57 , Issue 1 , February 1967 , pp. 9 - 17
Copyright
Copyright © Cambridge University Press 1967

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Davies, S. F. M., Joyner, L. P. & Kendall, S. B. (1962). Coccidiosis. Edinburgh: Oliver and Boyd.Google Scholar
van Doornink, W. M. & Becker, E. R. (1957). Transport of sporozoites of Eimeria necatrix in macrophages. J. Parasit. 43, 40–4.CrossRefGoogle Scholar
Garnham, P. C. (1950). The liver in relation to protozoal infections. Trans. R. Soc. trop. Med. Hyg. 43, 649–58.CrossRefGoogle Scholar
Gesner, B. M. (1965). The ‘life history’ and functions of lymphocytes. In Zweifach., B. W., Grant, L. & McCluskey, R. T.: The Inflammatory Process. New York and London: Academic Press.Google Scholar
Jackson, A. R. (1964). The isolation of viable coccidial oocysts. Parasitology 54, 87–93.CrossRefGoogle Scholar
McFarlane, D. (1944). Picro Mallory; An easily controlled regressive trichromic staining method. Stain Tech. 19, 29–37.CrossRefGoogle Scholar
Minning, W. (1936). Zur Entstehung der Kokzidienkunde in der Leber von Kaninchen. Z. ParasitKde 9, 61–72.CrossRefGoogle Scholar
Pérard, C. (1924). Recherches sur les coccidies et les coccidioses du lapin. Annls Inst. Pasteur—Paris 38, 953–76.Google Scholar
Ransom, B. H. & Cram, E. R. (1921). The course of migration of Ascaris larvae. Am. J. trop. Med. 1, 129–59.CrossRefGoogle Scholar
Reich, F. (1913). Das Kaninchencoccid E. stiedae (Lindemann, 1865). Arch. Protistenk. 28, 1–42.Google Scholar
Rose, M. E. (1958). The life cycle and development of Eimeria stiedae (Lindemann, 1865). Dissertation for the degree of Doctor of Philosophy in the University of Cambridge.Google Scholar
Shortt, H. E. & Cooper, W. (1947). Staining of microscopical sections containing protozoal parasites by a modification of McNamara's method. Trans. R. Soc. trop. Med. Hyg. 41, 427–28.Google Scholar
Siefried, C. (1927). Kaninchenkokzidiose (Coccidiosis cuniculi). Ergeben. allg. Path. Anat. 32, 509–20.Google Scholar
Smetana, H. (1933 a). Coccidiosis of the liver of rabbits; experimental study of the mode of infection of the liver by sporozoites. Archs Path. 15, 330–39.Google Scholar
Smetana, H. (1933 b). Coccidiosis of the liver of rabbits; experimental study of the histogenisis of coccidiosis of the liver. Archs Path. 15, 516–36.Google Scholar
von Wasielewski, T. (1904). Studien und Microphotogramme zur Kenntnis der Pathogenen Protozoen. 1. Untersuchungen über den bau die Entwicklung und über der pathogene Bedeutung der Coccidien. Leipzig: J. A. Barth.Google Scholar