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Ribonucleic acid synthesis in Plasmodium knowlesi maintained both in vivo and in vitro

Published online by Cambridge University Press:  06 April 2009

P. I. Trigg ,
P. G. Shakespeare ,
Susan J. Burt  and
Sally I. Kyd
P. I. Trigg
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
P. G. Shakespeare
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Susan J. Burt
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Sally I. Kyd
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Extract

RNA extracted from purified parasites of Plasmodium knowlesi was fractionated using agarose gel electrophoresis. Preparations from parasites grown both in vivo and in vitro contained species of RNA with sedimentation coefficients of 4·0S, 5·0S, 16·6S, 24·2S, 31·4S, 38·0S and 48·3S. There was less RNA present in parasites grown in vitro than the equivalent stage parasites grown in vivo but the proportional amounts of the various species of RNA was similar in both cases. It is suggested that the 24·2S and 16·6S species of RNA are ribosomal and that the high molecular weight 31·4S, 38·0S and 48·0S species are ribosomal precursors. Ribosomal RNA synthesis occurs throughout the cell cycle during growth from the ring to the schizont stage; maximum incorporation of [H3]-adenosine occurs at the late trophozoite stage before nuclear division.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 2 , October 1975 , pp. 199 - 209
Copyright
Copyright © Cambridge University Press 1975

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References

Acheson, N. H., Buetti, E., Scherrer, K. & Weil, R. (1971). Transcription of the polyoma virus genome synthesis and cleavage of giant late polyoma-specific RNA. Proceedings of the National Academy of Sciences 68, 2231–5.CrossRefGoogle ScholarPubMed
Aikawa, M. (1966). The fine structure of the erythrocytic stages of three avian malarial parasites, Plasmodium fallax, P. lophurae and P. cathemerium. American Journal of Tropical Medicine and Hygiene 15, 448–71.CrossRefGoogle ScholarPubMed
Aikawa, M. (1971). Plasmodium: The fine structure of malarial parasites. Experimental Parasitology 30, 284–3.CrossRefGoogle ScholarPubMed
Aikawa, M. & Beaudoin, R. L. (1968). Studies on nuclear division of a malarial parasite under pyrimethamine treatment. Journal of Cell Biology 39, 749–54.CrossRefGoogle ScholarPubMed
Aikawa, M., Cook, R. T., Sakoda, J. J. & Sprinz, H. (1969). Fine structure of the erythrocytic stages of Plasmodium knowlesi: A comparison between intracellular and free forms. Zeitschrift für Zellforschung und Mikroscopische Anatomie 100, 271–84.CrossRefGoogle ScholarPubMed
Bray, G. S. (1960). A single efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1, 279–85.CrossRefGoogle Scholar
Brown, I. N., Brown, K. N. & Hills, L. A. (1966). The separation of Plasmodium knowlesi from host cells. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 3.CrossRefGoogle Scholar
Click, R. E. & Tint, B. L. (1967). Comparative sedimentation rates of plant, bacterial and animal ribosomal RNA. Journal of Molecular Biology 25, 111–22.Google Scholar
Darnell, J. E. (1968). Ribonucleic acids from animal cells. Bacteriological Reviews 32, 262–90.Google Scholar
Dische, Z. (1955). Color reactions of nucleic acid components in The Nucleic Acids (ed. Chargaff, E., & Davidson, J. N.), p. 287. Academic Press: New York.Google Scholar
Fleck, A. & Munro, H. N. (1962). The precision of ultraviolet absorption measurements in the Schmidt-Tannhauser procedure for nucleic acid estimation. Biochimica et Biophysica Acta 55, 571–83.CrossRefGoogle Scholar
Fujisawa, T., Abe, S., Kawada, T., Satake, M. & Ogata, K. (1973). Studies on the processing of 45-S RNA in rat liver nucleolus with specific reference to 29–5 S RNA. Biochimica et Biophysica Acta 324, 226–40.Google Scholar
Gutteridge, W. E. & Trigg, P. I. (1970). Incorporation of radioactive precursors into DNA and RNA of Plasmodium knowlesi in vitro. The Journal of Protozoology 17, 89–96.Google Scholar
Hamilton, M. G. (1967). The molecular weight of the 30-S RNA of Jensen sarcoma ribosomes as determined by equilibrium centrifugation. Biochemica et Biophysica Acta 134, 473–5.CrossRefGoogle Scholar
Kjeldgaard, N. O. & Kurland, C. G. (1963). The distribution of soluble and ribosomal RNA as a function of growth rate. The Journal of Molecular Biology 6, 341–8.Google Scholar
Koroly, M. J. & Conner, R. L. (1974). The fate of RNA degradation products in starved cultures of Tetrahymena pyriformis W. The Journal of Protozoology 21, 169–77.CrossRefGoogle ScholarPubMed
Koln, J. & Feinberg, J. G. (1965). Electrophoresis on cellulose acetate. Shandon Instrument Applications, 11.Google Scholar
Leick, V. & Plesner, P. (1968). Formation of ribosomes in Tetrahymena pyriformis. Biochimica et Biophysica Acta 169, 398–408.CrossRefGoogle ScholarPubMed
Lewicki, P. P. & Sinskey, A. J. (1970). Precision of RNA separation by polyacrylamide gel electrophoresis. Analytical Biochemistry 33, 273–8.CrossRefGoogle ScholarPubMed
Loening, U. E. (1968). Molecular weights of ribosomal RNA in relation to evolution. The Journal of Molecular Biology 38, 355–65.CrossRefGoogle ScholarPubMed
Loening, U. E. (1969). The determination of the molecular weight of ribonucleic acid by polyacrylamide gel electrophoresis. The Biochemical Journal 113, 131–8.CrossRefGoogle Scholar
McConkey, E. H. & Hopkins, J. W. (1969). Molecular weights of some HeLa cell ribosomal RNA's. The Journal of Molecular Biology 39, 545–50.CrossRefGoogle ScholarPubMed
McIndoe, W. & Munro, H. N. (1967). Species of RNA from liver cell fractions separated on agarose gels. Biochemica et Biophysica Acta 134, 458–60.Google Scholar
Mita, T. (1965). Effects of actinomycin D on the RNA synthesis and the synchronous cell division of Tetrahymena pyriformis G.L. Biochimica et Biophysica Acta 103, 182–5.CrossRefGoogle Scholar
Mitchison, J. M. (1971). The Biology of the Cell Cycle, pp. 115–27. Cambridge University Press.Google Scholar
Penman, S. (1967). Ribonucleic acid metabolism in mammalian cells. The New England Journal of Medicine 276, 502–11.Google Scholar
Peterman, M. L. & Pavlovec, A. (1966). The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes. Biochemica et Biophysica Acta 114, 264–76.CrossRefGoogle Scholar
Prescott, D. M. (1964). The normal cell cycle. In Synchrony in Cell Division and Growth, pp. 71–108. (ed. E., Zeuthen). New York: Interscience Publishers Inc.Google Scholar
Rao, M. V. N. & Prescott, D. M. (1967). Micronuclear RNA synthesis in Paramecium caudatum. The Journal of Cell Biology 33, 281–5.Google Scholar
Shakespeare, P. G. & Trigg, P. I. (1973). Glucose catabolism by the simian malaria parasite Plasmodium knowlesi. Nature, London 241, 538–40.Google Scholar
Tokuyasu, K., Ilan, J. & Ilan, J. (1969). Biogenesis of ribosomes in Plasmodium berghei. Military Medicine 134, (Suppl.) 1032–8.CrossRefGoogle ScholarPubMed
Trigg, P. I. & Gutteridge, W. E. (1971). A minimal medium for the growth of Plasmodium knowlesi in dilution cultures. Parasitology 62, 113–23.CrossRefGoogle ScholarPubMed
Trigg, P. I. & Gutteridge, W. E. (1972). A rational approach to the serial culture of malaria parasites: evidence for a deficiency in RNA synthesis during the first cycle in vitro. Parasitology 65, 265–71.Google Scholar
Warhurst, D. C. & Williamson, J. (1968). Electrophoretic fractionation of ribonucleic acid from Plasmodium knowlesi. Transactions of the Royal Society of Tropical Medicine and Hygiene 62, 3–4.Google Scholar
Warhurst, D. C. & Williamson, J. (1970). Ribonucleic acid from Plasmodium knowlesi before and after chloroquine treatment. Chemical-Biological Interactions 2, 89–106.Google Scholar
Williamson, J. & Cover, B. (1966). Separation of blood-cell free trypanosomes and malaria parasites on a sucrose gradient. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 425–7.CrossRefGoogle Scholar