Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-24T11:57:42.978Z Has data issue: false hasContentIssue false
  • English
  • Français

Reversibility of the effects of the host immune response on the intestinal phase of Trichinella spiralis in the mouse, following transplantation to a new host

Published online by Cambridge University Press:  06 April 2009

M. W. Kennedy  and
R. G. Bruce
M. W. Kennedy
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
R. G. Bruce
Affiliation:
Department of Zoology, University of Glasgow, Glasgow G12 8QQ
Get access Rights & Permissions [Opens in a new window]

Summary

On day 10 of a primary infection of Trichinella spiralis in NIH mice, when adult worms were in the process of being expelled, their fecundity was markedly depressed. Upon transplantation to new host mice, the worms were able to establish successfully in their usual position in the anterior small intestine, even if originally recovered from the large intestine of mice expelling their worms, and these worms fully recovered their fecundity after 7 days in recipients. On day 5 of secondary and tertiary infections, immediately before loss of worms began, the adult worms were stunted and had a depressed fecundity. However, such worms, when transplanted, were able to establish in normal numbers and in a normal position in the intestine of recipient mice. Additionally, these worms were found to have developed a fecundity, and grown to a length, comparable to that of normal worms, but slightly less than that of control worms transplanted from day 7 of a primary infection, after 7 days in a new host. Electron microscopy showed that worms taken at the time of expulsion in primary and tertiary infections showed structural abnormalities, including large quantities of lipid in intestinal cells and somatic musculature and decreased quantities of stored glycogen. Upon transplantation to new host mice, these worms were found to have recovered a normal ultrastructural appearance within 7 days, although there was still evidence of a slightly elevated lipid and depressed glycogen content. It is concluded that immune expulsion of T. spiralis from mice does not involve irreversible damage to the worms.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 1 , February 1981 , pp. 39 - 48
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ballantyne, A. J., Sharpe, M. J. & Lee, D. L. (1978) Changes in the adenylate energy charge of Nippostrongylus brasiliensis and Nematodirus battus during the development of immunity to these nematodes in their hosts. Parasitology 76, 211–20.Google Scholar
Castro, G. A. (1976) Spatial and temporal integration of host responses to intestinal stages of Trichinella spiralis: retro- and prospective views. In Biochemistry of Parasites and Host-Parasite Relationships (ed. Van den Bossche, H.), pp. 343–358. Amsterdam: Elsevier/North-Holland Biomedical Press.Google Scholar
Denham, D. A. & Martinez, A. R. (1970) Studies with methyridine and Trichinella spiralis. 2. The use of the drug to study the rate of larval production in mice. Journal of Helminthology 44, 357–63.Google Scholar
Despommier, D. D., Campbell, W. C. & Blair, L. S. (1977) The in vivo and in vitro analysis of immunity to Trichinella spiralis in mice and rats. Parasitology 74, 109–19.Google Scholar
Despommier, D. D., Sukhdeo, M. & Meerovitch, E. (1978) Trichinella spiralis: site selection by the larva during the enteral phase of infection in mice. Experimental Parasitology 44, 209–15.CrossRefGoogle ScholarPubMed
Fatunmbi, O. O. (1978) Studies on some aspects of the development of Trichinella spiralis in mice. M.Sc. dissertation, University of Glasgow.Google Scholar
Gardiner, C. H. (1976) Habitat and reproductive behavior of Trichinella spirals. Journal of Parasitology 62, 865–70.CrossRefGoogle Scholar
Jenkins, D. C., Ogilvie, B. M. & McLaren, D. J. (1976) The effects of immunity and the mode of infection on the development of Nippostrongylus brasiliensis in rats. In Biochemistry of Parasites and Host-Parasite Relationships (ed. van den Bossche, H.), pp. 299–306. Amsterdam: Elsevier/North-Holland Biomedical Press.Google Scholar
Jenkins, D. C. & Phillipson, R. F. (1972) Evidence that the nematode Nippostrongylus brasiliensis can adapt and overcome the effects of host immunity. International Journal for Parasitology 2, 353–9.CrossRefGoogle ScholarPubMed
Kennedy, M. W. (1976) Kinetics of establishment and rejection of the enteral phase of a primary infection of Trichinella spiralis in the NIH strain mouse. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 285.Google Scholar
Kennedy, M. W. (1979) Effects of the host immune response on the intestinal phase of Trichinella Spiralis (Nematoda) in mice. Ph.D. dissertation, University of Glasgow.Google Scholar
Kennedy, M. W. (1980) Effects of the host immune response on the longevity, fecundity and position in the intestine, of Trichinella spiralis in mice. Parasitology 80, 49–60.Google Scholar
Kennedy, M. W., Wakelin, D. & Wilson, M. M. (1979) Transplantation of adult Trichinella spiralis between hosts: worm survival and immunological characteristics of the host- parasite relationship. Parasitology 78, 121–30.CrossRefGoogle ScholarPubMed
Kozlov, D. P. (1972) Repeated fertilization in Trichinella spiralis. Parasitologiya 6, 360–3. (In Russian, English abstract: Helminthological Abscracts 42, No. 1681, 1973).Google Scholar
Larsh, J. E. Jr (1963) Experimental trichiniasis. In Advances in Parasitology, vol. 1 (ed. Dawes, B.), pp. 213–86. London: Academic Press.Google Scholar
Lee, D. L. (1969) Nippostrongylus brasiliensis: some aspects of the fine structure and biology of the infective larva and the adult. In Nippostrongylus and Toxoplasma. Symposia of the British Society for Parasitology, vol. 7 (ed. Taylor, A. E. R.), pp. 3–16. Oxford: Blackwell Scientific Publications.Google Scholar
Love, R. J., Ogilvie, B. M. & McLaren, D. J. (1976) The immune mechanism which expels the intestinal stage of Trichinella spiralis from rats. Immunology 30, 7–15.Google Scholar
McCoy, O. R. (1931) Immunity of rats to reinfection with Trichinella spiralis. American Journal of Hygiene 14, 484–94.Google Scholar
Ogilvie, B. M. & Hockley, D. J. (1968) Effects of immunity on Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction, and morphology. Journal of Parasitology 54, 1073–84.CrossRefGoogle ScholarPubMed
Rappaport, I. & Wells, H. S. (1951) Studies in trichinosis. I. Immunity to reinfection following a single light infection. Journal of Infectious Diseases 88, 248–53.Google Scholar
Threadgold, L. T. (1967) The ultrastructure of the animal cell. In International Series of Monographs in Pure and Applied Biology-Zoology Division, vol. 37 (ed. Kerkut, G. A.), p. 313. Oxford: Pergamon Press.Google Scholar
Wakelin, D. (1978) Immunity to intestinal parasites. Nature, London 273, 617–20.CrossRefGoogle ScholarPubMed
Wakelin, D. & Lloyd, M. (1976) Immunity to primary and challenge infections of Trichinella spiralis in mice: a re-examination of conventional parameters. Parasitology 72, 173–82.Google Scholar