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The response to Schistosoma bovis in normal and T-cell deprived mice

Published online by Cambridge University Press:  06 April 2009

H. M. Murare ,
Alison Agnew ,
Marilyn Baltz ,
S. B. Lucas  and
M. J. Doenhoff
H. M. Murare
Affiliation:
Department of Medical Helminthology, London School of Tropical Hygiene and Medicine, Winches Farm Laboratories, 395 Hatfield Road, St Albans AL4 0XQ
Alison Agnew
Affiliation:
Department of Medical Helminthology, London School of Tropical Hygiene and Medicine, Winches Farm Laboratories, 395 Hatfield Road, St Albans AL4 0XQ
Marilyn Baltz
Affiliation:
Department of Medical Helminthology, London School of Tropical Hygiene and Medicine, Winches Farm Laboratories, 395 Hatfield Road, St Albans AL4 0XQ
S. B. Lucas
Affiliation:
Department of Medical Helminthology, London School of Tropical Hygiene and Medicine, Winches Farm Laboratories, 395 Hatfield Road, St Albans AL4 0XQ
M. J. Doenhoff*
Affiliation:
Department of Medical Helminthology, London School of Tropical Hygiene and Medicine, Winches Farm Laboratories, 395 Hatfield Road, St Albans AL4 0XQ
*
*Reprint requests to Dr M. J. Doenhoff.
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Summary

Normal and T-cell deprived mice have been compared in their response to infection with Schistosoma bovis. The deprived mice survived longer than comparably infected, immunologically intact controls, despite an increased longevity of the adult S. bovis worms in the former animals giving rise to higher tissue egg densities. The reduced pathology in deprived mice was due to inhibition of T-cell dependent granuloma formation around tissue-bound schistosome eggs, with concomitantly decreased tissue disruption as evidenced by smaller spleens and lower circulating transaminase concentrations. These observations on S. bovis contrast with the greater morbidity and earlier mortality induced by S. mansoni in T-cell deprived mice, the latter due to an hepatotoxic potential of S. mansoni eggs that is expressed in the absence of the host immune response. The absence of hepatocyte damage around S. bovis eggs in deprived mice indicates that this schistosome lacks such a toxin, and this could explain why during S. bovis infection synthesis of the two acute-phase proteins, complement C3 and serum amyloid P-component (SAP) here seemed less T-dependent than has previously been found during S. mansoni infection of mice. In a time-course experiment the hypergammaglobulinaemia induced by S. bovis, and the specific IgG antibody response against egg antigens were significantly T-cell dependent during the early stages of patency. Similarly, in most experiments assayed once between 9 and 11 weeks after S. bovis infection, deprived mice had significantly reduced hypergammaglobulinaemias, and reduced specific IgM and IgG antibody responses against both worm and egg antigens.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 3 , December 1987 , pp. 517 - 530
Copyright
Copyright © Cambridge University Press 1987

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References

Byram, J. E. & von Lichtenberg, F. (1977). Altered schistosome granuloma formation in nude mice. American Journal of Tropical Medicine and Hygiene 26, 944–56.CrossRefGoogle ScholarPubMed
Byram, J. E., Doenhoff, M. J., Musallam, R., Brink, L. H. & von Lichtenberg, F. (1979). Schistosoma mansoni infections in T-cell deprived mice, and the ameliorating effect of administering homologous chronic infection serum. II. Pathology. American Journal of Tropical Medicine and Hygiene 28, 274–85.CrossRefGoogle ScholarPubMed
Colten, H. R. & Einstein, L. P. (1976). Complement metabolism: cellular and humoral regulation. Transplantation Reviews 32, 3–24.Google ScholarPubMed
Doenhoff, M. J., Hassounah, O. A. & Lucas, S. B. (1985). Does the immunopathology induced by schistosome eggs potentiate parasite survival? Immunology Today 6, 203–6.CrossRefGoogle ScholarPubMed
Doenhoff, M. J., Hassounah, O., Murare, H., Bain, J. & Lucas, S. (1986). The schistosome egg granuloma: immunopathology in the service of host protection or parasite survival? Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 503–14.CrossRefGoogle ScholarPubMed
Doenhoff, M. J., Pearson, S., Dunne, D. W., Bickle, Q., Lucas, S., Bain, J., Musallam, R. & Hassounah, O. (1981). Immunological control of hepatotoxicity and parasite egg excretion in Schistosoma mansoni infections: stage specificity of the reactivity of immune serum in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 41–53.CrossRefGoogle ScholarPubMed
Doenhoff, M. J., Musallam, R., Bain, J. & McGregor, A. (1979). Schistosoma mansoni infections in T-cell deprived mice, and the ameliorating effects of administering homologous chronic infection serum. I. Pathogenesis. American Journal of Tropical Medicine and Hygiene 28, 260–73.CrossRefGoogle ScholarPubMed
Dunne, D. W., Bain, J., Lillywhite, J. & Doenhoff, M. J. (1984). The stage-, strain- and species-specificity of a Schistosoma mansoni egg antigen fraction (CEF6) with serodiagnostic potential. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 460–70.CrossRefGoogle ScholarPubMed
Dunne, D. W., Lucas, S., Bickle, Q., Pearson, S., Madgwick, L., Bain, J. & Doenhoff, M. J. (1981) Identification and partial purification of an antigen (ω1) from Schistosoma mansoni eggs which is putatively hepatotoxic in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 54–71.CrossRefGoogle Scholar
Engvall, E. & Perlmann, P. (1971). Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8, 871–4.CrossRefGoogle ScholarPubMed
Karman, A. (1955). A note on the spectrophotometric assay of glutamic oxalacetic transaminase in human blood serum. Journal of Clinical Investigation 34, 126–33.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
Lucas, S., Musallam, R., Bain, J., Hassounah, O., Bickle, Q. & Doenhoff, M. (1980). The pathological effects of immunosuppression of Schistosoma mansoni-infected mice, with particular reference to survival and hepatotoxicity after thymectomy and treatment with antithymocyte serum, and treatment with hydrocortisone acetate. Transactions of the Royal Society of Tropical Medicine and Hygiene 74, 633–43.CrossRefGoogle ScholarPubMed
McLaren, M. L., Draper, C. C., Roberts, R. M., Minter-Goedbloed, E., Ligthart, G. S., Teesdale, C. H., Amin, M. A., Omer, A. H. S., Bartlett, A. & Voller, A. (1978). Studies on the enzyme-linked immunosorbent assay (ELISA) test for Schistosoma mansoni infection. Annals of Tropical Medicine and Parasitology 72, 243–53.CrossRefGoogle Scholar
Mancini, G., Carbonara, O. A. & Heremans, J. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. International Journal of Immunochemistry 2, 235–4.CrossRefGoogle ScholarPubMed
Murare, H. M. & Doenhoff, M. J. (1987). Parasitological observations on Schistosoma bovis in normal and T-cell deprived mice. Parasitology 95, 507–16.CrossRefGoogle ScholarPubMed
Musallam, R., Bain, J., McGregor, A. & Doenhoff, M. (1980). Serum protein concentrations during Schistosoma mansoni infection in intact and T-cell deprived mice. II. Immunoglobulin G and antibodies specific for heterologous erythrocytes. Immunology 40, 343–52.Google ScholarPubMed
Pepys, M. B. (1975). Studies in vivo of cobra factor and murine C3. Immunology 28, 369–77.Google ScholarPubMed
Pepys, M. B. (1979). Isolation of serum amyloid P-component (protein SAP) in the mouse. Immunology 37, 637–41.Google ScholarPubMed
Pepys, M. B., Baltz, M., Musallam, R. & Doenhoff, M. J. (1980). Serum protein concentrations during Schistosoma mansoni infection in intact and T-cell deprived mice. I. The acute phase proteins, C3 and serum amyloid P-component (SAP). Immunology 39, 249–54.Google ScholarPubMed
Pepys, M. B., Dash, A. C., Fielder, A. H. L. & Mirjah, D. D. (1977). Isolation and study of murine C3. Immunology 33, 491–9.Google ScholarPubMed
Taylor, R. B. & Wortis, H. H. (1968). Thymus dependence of antibody response: variation with dose of antigen and class of antibody. Nature, London 220, 927–8.CrossRefGoogle ScholarPubMed
von Lichtenberg, F. (1962). Host response to eggs of S. mansoni. I. Granuloma formation in the unsensitized laboratory mouse. American Journal of Pathology 41, 711–31.Google ScholarPubMed
von Lichtenberg, F. (1964). Studies on granuloma formation. III. Antigen sequestration and destruction in the schistosome pseudotubercle. American Journal of Pathology 45, 75–93.Google ScholarPubMed
Warren, K. S. (1975). Hepatosplenic schistosomiasis mansoni: an immunologic disease. Bulletin of the New York Academy of Medicine 51, 545–55.Google ScholarPubMed