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Resistance against Schistosoma mansoni induced by highly irradiated infections: studies on species specificity of immunization and attempts to transfer resistance

Published online by Cambridge University Press:  06 April 2009

Q. D. Bickle ,
B. J. Andrews ,
M. J. Doenhoff ,
M. J. Ford  and
M. G. Taylor
Q. D. Bickle
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
B. J. Andrews
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
M. J. Doenhoff
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
M. J. Ford
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
M. G. Taylor
Affiliation:
Department of Medical Helminthology, Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans AL4 OXQ
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Extract

Significant levels of resistance against Schistosoma mansoai challenge were developed by mice exposed to highly irradiated (20 krad.) cercariae of the homologous species (53–67%), whereas vaccination with S. bovis, S. haematobiurn or S.japonicuni failed to confer significant levels of resistance (–5–12%), thus confirming the specificity ofthe immunizing procedure. Attempts to transfer resistance to naive recipients by injection of serum and of spleen or lymph node cells from donor mice vaccinated with highly irradiated cercariae were largely unsuccessful. However, significant levels of resistance could be transferred to mice by injection of serum from rabbits exposed to irradiated cercariae. Comparable levels of resistance were conferred by injection of serum at the time of challenge (34–69%) or 5–6 days later (31–56%). Tn contrast, sera from rabbits injected with solubleegg antigen or homogenized cercariae failed to confer protection upon recipient mice. Sera from vaccinated mice, vaccinated rabbits and antigen-injected rabbits all caused cell adherence to skin-transformed schistosomula hut neither the level of adherence nor the serum titre correlated with the ability to confer protection to mice.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 301 - 312
Copyright
Copyright © Cambridge University Press 1985

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References

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