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Resistance against migrating Ascaris suum larvae in pigs immunized with infective eggs or adult worm antigens

Published online by Cambridge University Press:  12 July 2001

F. J. SERRANO ,
D. REINA ,
E. FRONTERA ,
A. ROEPSTORFF  and
I. NAVARRETE
F. J. SERRANO
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Avda de la Univerdidad s/n, 10071 Cáceres, Spain
D. REINA
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Avda de la Univerdidad s/n, 10071 Cáceres, Spain
E. FRONTERA
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Avda de la Univerdidad s/n, 10071 Cáceres, Spain
A. ROEPSTORFF
Affiliation:
Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Ridebanevej 3, DK-1870 Frederiksberg C, Denmark
I. NAVARRETE
Affiliation:
Parasitology Section, Department of Medicine and Animal Health, Facultad de Veterinaria, Universidad de Extremadura, Avda de la Univerdidad s/n, 10071 Cáceres, Spain
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Abstract

Resistance to Ascaris suum infections was investigated in 8- and 15-week-old Iberian pigs. Groups of 3 or 5 pigs were immunized weekly for 6 weeks with antigens of adult A. suum: a 97 kDa body wall (BW) fraction, a 42 kDa fraction of pseudocoelomic fluid (PF) or a 14 kDa PF-fraction; or were inoculated with increasing doses of infective eggs (500–20000), with or without abbreviation by pyrantel pamoate. All immunized pigs and unimmunized control pigs, were challenged with 10000 infective eggs 7 days after the last immunization. The number of liver lesions and lung larvae was substantially lower in the older pigs than in the younger ones 7 days after challenge, but the resistance in immunized pigs of both age groups was similar in comparison to the challenge controls of the same age. The highest degree of resistance against lung larvae was observed in pigs immunized with A. suum eggs (97–99%). The pigs immunized with the 14 kDa and 42 kDa PF-fractions were also well protected (67–93%), while no protection was produced by the 97 kDa BW fraction (0–49%). The reduction of white spots following immunization was less evident, with a maximum of 82% reduction in egg-inoculated young pigs.

Keywords

Ascaris suumswineimmunityantigens
Type
Research Article
Information
Parasitology , Volume 122 , Issue 6 , June 2001 , pp. 699 - 707
Copyright
© 2001 Cambridge University Press

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