Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-28T04:58:34.807Z Has data issue: false hasContentIssue false
  • English
  • Français

The release of membrane antigens into culture by adult Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

J. R. Kusel ,
P. E. Mackenzie  and
D. J. McLaren
J. R. Kusel
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
P. E. Mackenzie
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
D. J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
Get access Rights & Permissions [Opens in a new window]

Extract

Antigens sharing determinants with surface membranes and soluble proteins of adult Schistosoma mansoni have been detected in culture media after incubation of radioactively labelled worms. The relative quantities of these antigens were measured with specific antisera raised in rabbits and with serum from an immune rhesus monkey. It was found that 12–16% of TCA-precipitable radioactivity in the culture medium consisted of membrane antigens and 6–8% consisted of antigens sharing determinants with proteins found in the soluble fraction of adult worms. Over half the membrane antigens were present in particulate form, while other antigens were present in solution. Surface labelling the adult worms with [125I]confirmed that some of the particles in the culture medium were derived from the surface membrane of the adult worm and electron microscope examination of such particles showed that large membrane fragments were present. These results support the hypothesis that anti-bodies against schistosome membrane antigens are induced by particulate membrane antigens released by the parasite.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 2 , October 1975 , pp. 247 - 259
Copyright
Copyright © Cambridge University Press 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Avrameus, S., Taudou, B. & Chuilon, S. (1969). Glutaraldehyde, cyanuric chloride and tetra-azotised O-dianisidine as coupling reagents. Immunochemistry 6, 67–76.CrossRefGoogle Scholar
Clegg, J. A. & Smithers, S. R. (1972). The effects of immune rhesus monkey serum of schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–98.CrossRefGoogle ScholarPubMed
Dresser, D. W. & Mitchison, N. A. (1968). The mechanism of immunological paralysis. Advances in Immunology 8, 129–81.CrossRefGoogle ScholarPubMed
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 13–25.CrossRefGoogle ScholarPubMed
Hsu, S. Y., Li & Hsu, H. F. (1963). Further studies on rhesus monkeys immunized against Schistosoma japonicum by administration of cercariae of the Formosan strain. Zeitschrift für Tropenmedizin und Parasitologie 14, 506–12.Google Scholar
Kusel, J. R. (1972). Protein composition and protein synthesis in the surface membranes of Schistosoma mansoni. Parasitology 65, 55–69.CrossRefGoogle ScholarPubMed
Kusel, J. R., Sher, F. A., Perez, H., Clegg, J. A. & Smithers, S. R. (1975). The use of radioactive isotopes in the study of specific schistosome membrane antigens. Isotopes and Radiation in Parasitology IV, pp. 127–43. International Atomic Energy Agency: Vienna.Google Scholar
Levine, D. M. & Kagan, I. G. (1960). Studies on the immunology of schistosomiasis by vaccination and passive transfer. Journal of Parasitology 46, 787–92.CrossRefGoogle ScholarPubMed
Murrell, K. D. & Clay, B. (1972). In vitro detection of cytotoxic antibodies to Schistosoma mansoni schistosomules. American Journal of Tropical Medicine and Hygiene 21, 569–77.CrossRefGoogle ScholarPubMed
Murrell, K. D., Vannier, W. E. & Ahmed, A. (1974). Schistosoma mansoni. Antigenic heterogeneity of excretions and secretions. Experimental Parasitology 36, 316–30.CrossRefGoogle ScholarPubMed
Parish, C. R. (1972). Preferential induction of cell mediated immunity by chemically modified sheep erythrocytes. European Journal of Immunology 2, 143–51.CrossRefGoogle ScholarPubMed
Perez, H., Clegg, J. A. & Smithers, S. R. (1974). Acquired immunity to Schistosoma mansoni in the rat. Measurement of immunity by the lung recovery technique. Parasitology 69, 349–59.CrossRefGoogle ScholarPubMed
Perez, H. & Terry, R. J. (1973). The killing of adulti Schistosoma mansoni in vitro in the presence of antisera to host antigenic determinants and peritoneal cells. International Journal for Parasitology 3, 499–503.CrossRefGoogle Scholar
Phillips, D. R. & Morrison, M. (1970). The arrangement of proteins in the human erythro-cyte membrane. Biochemical and Biophysical Research Communications 40, 284–9.CrossRefGoogle Scholar
Riggs, A. D., Bourgeois, S., Newby, R. F. & Cohn, M. (1968). DNA binding of the lac repressor. Journal of Molecular Biology 34, 365–8.CrossRefGoogle ScholarPubMed
Sadun, E. H. & Lin, S. S. (1959). Studies on the host-parasite relationship of Schistosoma japonicum. IV. Resistance acquired by infection, vaccination, and by injection of immune serum in monkeys, rabbits and mice. Journal of Parasitology 45, 543–7.CrossRefGoogle ScholarPubMed
Sher, F. A., Mackenzie, P. E. & Smithers, S. R. (1974). Decreased recovery of invading parasites from the lungs as a parameter of acquired immunity to schistomiasis in the laboratory mouse. Journal of Infectious Diseases 130, 626–33.CrossRefGoogle Scholar
Smithers, S. R. & Terry, R. J. (1969). The Immunology of Schistosomiasis. In Advances in Parasitology vol. 7 (ed. Dawes, B.), pp. 41–93. New York and London: Academic Press.Google Scholar