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Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)

Published online by Cambridge University Press:  11 August 2014

J. P. DUBEY ,
M. HILALI ,
E. VAN WILPE ,
S. K. VERMA ,
R. CALERO-BERNAL  and
A. ABDEL-WAHAB
J. P. DUBEY*
Affiliation:
U. S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA
M. HILALI
Affiliation:
Parasitology Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
E. VAN WILPE
Affiliation:
Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, South Africa
S. K. VERMA
Affiliation:
U. S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA
R. CALERO-BERNAL
Affiliation:
U. S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA
A. ABDEL-WAHAB
Affiliation:
Parasitology Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
*
* Corresponding author: USDA, ARS, APDL, BARC-East, Building 1001, Beltsville, MD 20705, USA. E-mail: [email protected]
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Summary

Four valid species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis, Sarcocystis buffalonis, Sarcocystis levinei and Sarcocystis dubeyi. Here, we redescribe structure of S. fusiformis sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9 μm thick, depending on the section and the technique. In 5 μm paraffin-embedded sections, the sarcocyst wall was indistinct, 2–5 μm thick and appeared smooth. In 1 μm plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2·5–5·2 μm thick and had branched villar protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the sarcocyst wall. On each vp there were uniform papillomatous structures that were 100 nm wide. By TEM, vp were up to 6 μm long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11·2 to 16·8 μm in length. By TEM, bradyzoites had a very long (10 μm) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of S. fusiformis from similar sarcocysts in domestic and wild ruminants.

Keywords

Sarcocystis fusiformissarcocystwater buffalo (Bubalus bubalis)electron microscopyultrastructure
Type
Research Article
Information
Parasitology , Volume 142 , Issue 2 , February 2015 , pp. 385 - 394
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Creative Commons
This is a work of the U.S. Government and is not subject to copyright protection in the United States.
Copyright
Copyright © Cambridge University Press 2014

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