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Recombinant protein Bd37 protected gerbils against heterologous challenges with isolates of Babesia divergens polymorphic for the bd37 gene

Published online by Cambridge University Press:  13 October 2006

K. HADJ-KADDOUR ,
B. CARCY ,
A. VALLET ,
S. RANDAZZO ,
S. DELBECQ ,
J. KLEUSKENS ,
T. SCHETTERS ,
A. GORENFLOT  and
E. PRECIGOUT
K. HADJ-KADDOUR
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
B. CARCY
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
A. VALLET
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
S. RANDAZZO
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
S. DELBECQ
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
J. KLEUSKENS
Affiliation:
Department of Parasitology, Intervet International B.V., Boxmeer, The Netherlands
T. SCHETTERS
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France Department of Parasitology, Intervet International B.V., Boxmeer, The Netherlands
A. GORENFLOT
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
E. PRECIGOUT
Affiliation:
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’, UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France
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Abstract

The Bd37gene encoding for a glycosyl-phosphatidyl-inositol anchored protein of Babesia divergens displays genetic polymorphisms among isolates. Five major polymorphic groups (clades) were shown by PCR-RFLP among different B. divergens isolates. Each group has been characterized according to a reference Bd37 gene (Rouen87, W8843, Y5, 6303E and 1705B). Recombinant (GST fusion) protein (Bd37r) expressed from the Bd37 gene, was used as antigen in a saponin-based formulation and was able to protect gerbils, after 2 injections at low dose vaccine concentration (1 μg per dose), against a virulent challenge with the B. divergens Rouen87 isolate. In spite of polymorphism of Bd37 gene, Bd37r induced complete immunoprotection against challenges with each of the 5 reference isolate groups defined by PCR-RFLP.

Keywords

Babesiosisrecombinant proteinheterologous vaccineBabesia divergens
Type
Research Article
Information
Parasitology , Volume 134 , Issue 2 , February 2007 , pp. 187 - 196
Copyright
2006 Cambridge University Press

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