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rDNA phylogeny of Intrapredatorus barri (Microsporida: Amblyosporidae) parasitic to Culex fuscanus Wiedemann (Diptera: Culicidae)

Published online by Cambridge University Press:  12 July 2001

F. NILSEN  and
W.-J. CHEN
F. NILSEN
Affiliation:
Institute of Marine Research, Department of Aquaculture, P. O. Box 1870, N-5817 Bergen, Norway
W.-J. CHEN
Affiliation:
Department of Public Health and Parasitology, College of Medicine, Chang Gung University, Kwei-San, Tao-Yuan 33332, Taiwan
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Abstract

Intrapredatorus barri, a microsporidian parasite of the predator mosquito Culex fuscanus Wiedemann has been shown to be heterosporous. In many features this species has been reported to be similar to Amblyospora trinus, another microsporidian parasite of a different predator mosquito which was thus proposed for reassignation to the genus Intrapredatorus. In this report small subunit rDNA has been sequenced from I. barri and phylogenetic analysis of 42 microsporidia sequences has been performed.Nucleotide sequence data reported in this paper are available in the Gen Bank™ database under the accession number AY013359. The molecular data show that I. barri can not be a member of the genus Amblyospora. Furthermore, the phylogenetic analysis shows, with high confidence, that the Amblyospora spp. sequences in the present study are not monophyletic.

Keywords

Culex fuscanusIntrapredatorus barriphylogenyssRNA sequence
Type
Research Article
Information
Parasitology , Volume 122 , Issue 6 , June 2001 , pp. 617 - 623
Copyright
© 2001 Cambridge University Press

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References

ANDREADIS, T. G. (1989). Host specificity of Amblyospora connecticus (Microsporida: Amblyosporidae), a polymorphic microsporidian parasite of Aedes cantator (Diptera: Culicidae). Journal of Medical Entomology 26, 140–145.CrossRefGoogle Scholar
ANDREADIS, T. G. (1994a). Ultrastructural characterization of meiospores of six new species of Amblyospora (Microspora: Amblyosporidae) from northern Aedes (Diptera: Culicidae) mosquitoes. Journal of Eukaryotic Microbiology 41, 147–154.Google Scholar
ANDREADIS, T. G. (1994b). Host range tests with Edhazardia aedis (Microsporida: Culicosporidae) against northern nearctic mosquitoes. Journal of Invertebrate Pathology 64, 46–51.Google Scholar
ANDREADIS, T. G. (1999). Epizootiology of Amblyospora stimuli (Microsporida: Amblyosporidae) infections in field populations of a univoltine mosquito, Aedes stimulans (Diptera: Culicidae), inhabiting a temporary vernal pool. Journal of Invertebrate Pathology 74, 198–205.CrossRefGoogle Scholar
AVERY, S. W. & UNDEEN, A. H. (1990). Horizontal transmission of Parathelohania anopheles to the copepod, Microcyclops varicans and the mosquito, Anopheles quadrimaculatus. Journal of Invertebrate Pathology 56, 98–105.CrossRefGoogle Scholar
BAKER, M. D., VOSSBRINCK, C. R., MADDOX, J. V. & UNDEEN, A. H. (1994). Phylogenetic relationships among Vairimorpha and Nosema species (Microspora) based on ribosomal RNA sequence data. Journal of Invertebrate Pathology 64, 100–106.CrossRefGoogle Scholar
BAKER, M. D., VOSSBRINCK, C. R., BECNEL, J. J. & ANDREADIS, T. G. (1998). Phylogeny of Amblyospora (Microsporida: Amblyosporidae) and related genera based on small subunit ribosomal DNA data: a possible example of host parasite cospeciation. Journal of Invertebrate Pathology 71, 199–206.CrossRefGoogle Scholar
BECNEL, J. J. (1992). Horizontal transmission and subsequent development of Amblyospora californica (Microsporida: Amblyosporidae) in the intermediate and definitive hosts. Diseases of Aquatic Organisms 13, 17–28.CrossRefGoogle Scholar
BECNEL, J. J. & ANDREADIS, T. G. (1998). Amblyospora salinaria n. sp. (Microsporida: Amblyosporidae), parasite of Culex salinarius (Diptera: Culicidae): its life cycle stages in an intermediate host. Journal of Invertebrate Pathology 71, 258–262.Google Scholar
BECNEL, J. J. & SWEENEY, A. W. (1990). Amblyospora trinus n. sp. (Microsporida: Amblyosporidae) in the Australian mosquito Culex halifaxi (Diptera: Culicidae). Journal of Protozoology 37, 584–592.Google Scholar
BECNEL, J. J., HAZARD, E. I., FUKUDA, T. & SPRAGUE, V. (1987). Life cycle of Culicospora magna (Kudo, 1920) (Microsporida: Culicosporidae) in Culex restuans Theobald with special reference to sexuality. Journal of Protozoology 34, 313–322.CrossRefGoogle Scholar
BECNEL, J. J., SPRAGUE, V., FUKUDA, T. & HAZARD, E. I. (1989). Development of Edhazardia aedis (Kudo, 1930) N. G., N. Comb. (Microsporida: Amblyosporidae) in the mosquito Aedes aegypti (L.) (Diptera: Culicidae). Journal of Protozoology 36, 119–130.Google Scholar
CASTILLO, J. M. (1980). Microsporidian pathogens of Culicidae (Mosquitos). In Bibliography on Pathogens of Medically Important Arthropods: 1980 (ed. Roberts, D. W. & Castillo, J. M.). Bulletin of World Health Organization (Suppl.) 58, 33–46.
CHEN, W. J. (1998). A microsporidium of the predacious mosquito Culex fuscanus Wiedemann (Diptera: Culicidae) from southern Taiwan. Journal of Invertebrate Pathology 71, 179–181.CrossRefGoogle Scholar
CHEN, W. J., KUO, T. L. & WU, S. T. (1998). Development of a new microsporidian parasite, Intrapredatorus barri n.g., n. sp. (Microsporida: Amblyosporidae) from the predacious mosquito Culex fuscanus Wiedemann (Diptera: Culicidae). Parasitology International 47, 183–193.Google Scholar
CHENEY, S. A., LAFRANCHI-TRISTEM, N. J. & CANNING, E. U. (2000). Phylogenetic relationships of Pleistophora-like microsporidia based on small subunit ribosomal DNA sequences and implications for the source of Trochipleistophora hominis infections. Journal of Eukaryotic Microbiology 47, 280–287.CrossRefGoogle Scholar
GARCIA, J. J. & BECNEL, J. J. (1994). Eight new species of microsporidia (Microspora) from Argentine mosquitoes (Diptera: Culicidae). Journal of Invertebrate Pathology 64, 243–252.CrossRefGoogle Scholar
GARCIA, J. J., HAZARD, E. I. & FUKUDA, T. (1993). Light and electron microscopy studies on the development of Parathelohania anophelis (Microspora: Amblyosporidae) in female Anopheles quadrimaculatus (Diptera: Culicidae). Journal of Invertebrate Pathology 61, 85–89.CrossRefGoogle Scholar
GATEHOUSE, H. S. & MALONE, L. A. (1998). The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size. Journal of Invertebrate Pathology 71, 97–105.CrossRefGoogle Scholar
hazard, e. i. & chapman, h. c. (1977). Microsporidian pathogens of Culicidae (Mosquitos). In Pathogens of Medically Important Arthropods, (ed. Robert, D. W. & Strand, M. A.). Bulletin of World Health Organization (Suppl.) 55, 63–107.
HAZARD, E. I., FUKUDA, T. & BECNEL, J. J. (1984). Life cycle of Culicosporella lunata (Hazard & Savage 1970) Weiser, 1977 (Microspora) as revealed in the light microscope with a redescription of the genus and species. Journal of Protozoology 31, 385–391.CrossRefGoogle Scholar
LARSSON, R. (1988). Identification of microsporidian genera (Protozoa, Microspora)–a guide with comments on the taxonomy. Archiv für Protistenkunde 136, 1–37.CrossRefGoogle Scholar
MALONE, L. A. & MCIVOR, C. A. (1996). Use of nucleotide sequence data to identify a microsporidian pathogen of Pieris rapae (Lepidoptera, Pieridae). Journal of Invertebrate Pathology 68, 231–238.CrossRefGoogle Scholar
MALONE, L. A., BROADWELL, A. H., LINDRIDGE, E. T., MCIVOR, C. A. & NINHAM, J. A. (1994). Ribosomal RNA genes of two microsporidia, Nosema apis and Pavraia oncoperae are very variable. Journal of Invertebrate Pathology 64, 151–152.CrossRefGoogle Scholar
MOSER, B. A., BECNEL, J. J., MARUNIAK, J. & PATTERSON, R. (1998). Analysis of the ribosomal DNA sequences of the microsporidia Thelohania and Vairimorpha of fire ants. Journal of Invertebrate Pathology 72, 154–159.CrossRefGoogle Scholar
NILSEN, F. (1999). Small subunit rDNA phylogeny of Bacillidium sp. (Microsporidia, Mrazekiidae) infecting oligochaets. Parasitology 118, 553–558.Google Scholar
NILSEN, F. (2000). Small subunit ribosomal DNA phylogeny of microsporidia with particular reference to genera that infect fish. Journal of Parasitology 86, 128–133.CrossRefGoogle Scholar
NILSEN, F., ENDRESEN, C. & HORDVIK, I. (1998). Molecular phylogeny of microsporidians with particular reference to species that infect muscles of fish. Journal of Eukaryotic Microbiology 45, 529–537.CrossRefGoogle Scholar
SPRAGUE, V., BECNEL, J. J. & HAZARD, E. I. (1992). Taxonomy of Phylum Microspora. Critical Reviews in Microbiology 18, 285–395.CrossRefGoogle Scholar
SWOFFORD, D. L. (2000). PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4. Sinauer Associates, Sunderland, Massachusetts.
VAN DE PEER, Y., NICOLAI, S., DE RIJK, P. & DE WACHTER, R. (1996). Database on the structure of small ribosomal subunit rRNA. Nucleic Acids Research 24, 86–91.CrossRefGoogle Scholar
VAN DE PEER, Y., ROBBRECHT, E., DEHOOG, S., CAERS, A., DERIJK, P. & DEWACHER, R. (1999). Database on the structure of small subunit ribosomal RNA. Nucleic Acids Research 27, 179–183.CrossRefGoogle Scholar
VOSSBRINCK, C. R., BAKER, M. D., DIDIER, E. S., DEBRUNNER-VOSSBRINCK, B. A. & SHADDUCK, J. A. (1993). Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: Species identification and phylogenetic construction. Journal of Eukaryotic Microbiology 40, 354–362.CrossRefGoogle Scholar
ZHU, X., WITTNER, M., TANOWITZ, H. B., CALI, A. & WEISS, L. M. (1994). Ribosomal RNA sequences of Enterocytozoon bienusi, Septata intestinalis and Ameson michaelis: Phylogenetic construction and structural correspondence. Journal of Eukaryotic Microbiology 41, 204–209.CrossRefGoogle Scholar