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A rapid chromatographic method for elimination of fungal contamination in in vitro cultures of Leishmania spp

Published online by Cambridge University Press:  06 April 2009

B. M. Okot-Kotber
B. M. Okot-Kotber
Affiliation:
International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi
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Extract

It was demonstrated that antimycotic agents are unsuitable for elimination of mycotic contamination in in vitro cultures of Leishmania spp. Ion-exchange chromatography was designed to separate the protozoan parasites from the contaminating yeast or fungal cells based on their surface net charges. The method was found to be efficient and rapid, and would also reduce bacterial contamination in cultures which have been exposed to both contaminants Viability of the cells has been shown to be unaltered by this treatment as demonstrated by their ability to multiply rapidly in culture medium, as was seen in the controls.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 1 , August 1985 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Brazil, R. P. (1978). Isolation of intracellular stages of Leishmania mexicana amazonensis using cellulose column. Annals of Tropical Medicine and Parasitology 72, 579–80.Google Scholar
Cohen, B. A. J., Looij, B. J. & Wittner, M. (1977). 5-Fluorocytosine a valuable fungicidal agent in specific culture media. Annales de la Société Belge de Médecine Tropicale 57, 55–6.Google ScholarPubMed
Delektorskii, V. V., Tishchenko, L. D., Alita, O. V., Safianova, V. M. & Emelianova, L. P. (1982). Electronno-mikroskopicheskoe izuchenie izmenenii ultrastruktury promastigot L. brazili ensis pri vozdeistivii monomisinom amfogliukaminom. (Comparative electron microscopic study of the ultrastructural changes in L. braziliensis promastigotes exposed to monomycin and amphoglucamine.) Meditsinkaya Parazitoiogiya (Moskva) 60, 62–5.Google Scholar
Hart, D. T., Vickerman, K. & Coombs, G. H. (1981). A quick, simple method for purifying Leishmania mexicana amastigotes in large numbers. Parasitology 82, 345–55.Google Scholar
Homewood, C. A. & Neame, K. D. (1976). A comparison of methods used for the removal of white blood cells from malaria-infected blood. Annals of Tropical Medicine and Parasitology 70, 249–51.Google Scholar
Howard, R. J., Smith, P. M. & Mitchell, G. F. (1978). Removal of leukocytes from red cells in Plasmodium berghei-infected mouse blood and purification in schizont-infected cells. Annals of Tropical Medicine and Parasitology 72, 573–5.CrossRefGoogle ScholarPubMed
Infante, R. B., Hernandez, A. G., Riggione, F. & Dawidowicz, K. (1980). A new method for the partial purification of leishmania amastigotes from cutaneous lesions. Parasitology 80, 105–12.CrossRefGoogle ScholarPubMed
Kimber, C. D., Evans, D. A., Robinson, B. L. & Peters, W. (1981). Control of yeast contamination with 5-fluorocytosine in in vitro cultivation of Leishmania spp. Annals of Tropical Medicine and Parasitology 75, 453–4.Google Scholar
Lanham, S. M. & Godfrey, D. G. (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Experimental Parasitology 28, 521–34.CrossRefGoogle ScholarPubMed
New, R. R. C., Chance, M. L. & Heath, S. (1981). Antileishmanial activity of amphotericin and other antifungal agents entrapped in liposomes. Journal of Antimicrobial Chemotherapy 8, 371–81.CrossRefGoogle ScholarPubMed
Osisanya, J. O. S., Gould, S. & Warhurst, D. C. (1980). Elimination of yeast contamination from malaria cultures using 5-fluorocytosine. Annals of Tropical Medicine and Parasitology 74, 559.CrossRefGoogle ScholarPubMed