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A rapid chromatographic method for elimination of fungal contamination in in vitro cultures of Leishmania spp

Published online by Cambridge University Press:  06 April 2009

B. M. Okot-Kotber
B. M. Okot-Kotber
Affiliation:
International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi
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Extract

It was demonstrated that antimycotic agents are unsuitable for elimination of mycotic contamination in in vitro cultures of Leishmania spp. Ion-exchange chromatography was designed to separate the protozoan parasites from the contaminating yeast or fungal cells based on their surface net charges. The method was found to be efficient and rapid, and would also reduce bacterial contamination in cultures which have been exposed to both contaminants Viability of the cells has been shown to be unaltered by this treatment as demonstrated by their ability to multiply rapidly in culture medium, as was seen in the controls.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 1 , August 1985 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1985

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References

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