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Rapid and sensitive identification of Neospora caninum by in vitro amplification of the internal transcribed spacer 1

Published online by Cambridge University Press:  26 March 2010

O. J. M. Holmdahl  and
J. G. Mattsson
O. J. M. Holmdahl
Affiliation:
Department of Parasitology, Swedish University of Agricultural Sciences and the National Veterinary Institute, PO Box 7073, S-750 07, Uppsala, Sweden Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences, PO Box 7019, S-750 07 Uppsala, Sweden
J. G. Mattsson
Affiliation:
Department of Parasitology, Swedish University of Agricultural Sciences and the National Veterinary Institute, PO Box 7073, S-750 07, Uppsala, Sweden
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Summary

Neospora caninum and N. caninum-like organisms are cyst-forming coccidian parasites known to cause neuromuscular disorders in dogs and abortion in cattle. In this article we report on the use of the polymerase chain reaction (PCR) for the detection of DNA from N. caninum. After determining the sequence of the internal transcribed spacer 1 (ITSl) of N. caninum and Toxoplasma gondii, and part of the sequences for & species of Sarcocystis, we designed a primer set for the amplification of a 279-base-pair fragment of ITSl from N. caninum. The PCR system made possible the specific detection of 5 N. caninum organisms and no amplification was observed from any of the other cyst-forming coccidia tested, including the closely related T. gondii. Furthermore, we were also able to demonstrate the presence of N. caninum in brain and lung tissue samples from experimentally infected mice. Our data also link the 5-8S rRNA gene for T. gondii and N. caninum to the 16S-like rRNA gene, within the rDNA unit.

Keywords

polymerase chain reactionNeospora caninumToxoplasma gondiiinternal transcribed spacerrRNA
Type
Research Article
Information
Parasitology , Volume 112 , Issue 2 , February 1996 , pp. 177 - 182
Copyright
Copyright © Cambridge University Press 1996

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