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RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis

Published online by Cambridge University Press:  05 December 2003

E. MARTINEZ ,
V. ALONSO ,
A. QUISPE ,
M. C. THOMAS ,
R. ALONSO ,
J. E. PIÑERO ,
A. C. GONZÁLEZ ,
A. ORTEGA  and
B. VALLADARES
E. MARTINEZ
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
V. ALONSO
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
A. QUISPE
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
M. C. THOMAS
Affiliation:
Unidad Mixta de Investigaciones Médicas. Hospital Clínico Universitario San Cecilio. Dr Oloriz, 16, CP18012 Granada. Spain
R. ALONSO
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
J. E. PIÑERO
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
A. C. GONZÁLEZ
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
A. ORTEGA
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
B. VALLADARES
Affiliation:
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, CP38271 La Laguna, Tenerife, Spain
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Abstract

The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively.

Keywords

RAPDPCRprimerLeishmania braziliensis
Type
Research Article
Information
Parasitology , Volume 127 , Issue 6 , December 2003 , pp. 513 - 517
Copyright
2003 Cambridge University Press

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