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Quinanil as a Bactericidal Agent in the Isolation of an Insect Flagellate

Published online by Cambridge University Press:  06 April 2009

G. N. Boxhall ,
F. C. Happold  and
Ll. Lloyd
G. N. Boxhall
Affiliation:
From the Departments of Pathology and Bacteriology and Department of Zoology, University of Leeds
F. C. Happold
Affiliation:
From the Departments of Pathology and Bacteriology and Department of Zoology, University of Leeds
Ll. Lloyd
Affiliation:
From the Departments of Pathology and Bacteriology and Department of Zoology, University of Leeds
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Extract

A flagellate from Polietes lardaria, closely allied to Herpetomonas muscarum, has been repeatedly isolated in bacteria-free culture by the use of quinanil after numerous attempts to isolate it by other methods with one exception had failed. The flagellates were exposed to 1/10,000 or 1/100,000 quinanil for 2–4 or more hours and cultivated in Locke serum agar, the medium for the first subculture containing quinanil in concentrations of 1/10,000 or 1/50,000. The proofs of the purity of the cultures were exhaustive.

We are greatly indebted to Dr St John-Brooks and the National Collection of Type Cultures for certain of the flagellates isolated by Noguchi and Tilden. One of us (F. C. H.) has also been in receipt of grants from the Medical Research Council, and he would like to take this opportunity of expressing his thanks to that body.

Type
Research Article
Information
Parasitology , Volume 26 , Issue 1 , April 1934 , pp. 44 - 48
Copyright
Copyright © Cambridge University Press 1934

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