Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-23T07:56:17.540Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein antigens in the cyst fluid of Taenia saginata cysticerci

Published online by Cambridge University Press:  06 April 2009

G. W. P. Joshua ,
L. J. S. Harrison  and
M. M. H. Sewell
G. W. P. Joshua
Affiliation:
University of Edinburgh, Department of Tropical Animal Health, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG
L. J. S. Harrison
Affiliation:
University of Edinburgh, Department of Tropical Animal Health, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG
M. M. H. Sewell
Affiliation:
University of Edinburgh, Department of Tropical Animal Health, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG
Get access Rights & Permissions [Opens in a new window]

Summary

Taenia saginata cyst fluid proteins from 4, 8, 12 and 16-week-old cysticerci were analysed by a combination of direct I radio-isotope labelling, immunoprecipitation using a panel of sera from infected cattle infected with T. saginata and SDS–PAGE. Protein antigens of 12, 14, 16, 20 and 26 kDa were identified in all of the cyst fluids examined. These were immunogenic and were precipitated by serum taken from cattle from 8 weeks after infection onwards and were therefore considered to be of diagnostic potential. A 185 kDa protein antigen found only in the cyst fluid of 4-week-old cysticerci and a 43 kDa protein antigen first detected in cyst fluid from 8-week-old cysticerci were also identified but were considered to be of more limited diagnostic potential due to their restricted presence. An apparently non-immunogenic 67 kDa protein, found in all the cyst fluids examined, may have been host serum albumin.

Keywords

Taenia saginata cysticercicyst fluidprotein antigens
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 463 - 467
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72, 248–54.CrossRefGoogle ScholarPubMed
Gallie, G. J. & Sewell, M. M. H. (1972). The survival of Cysticercus bovis in resistant calves. The Veterinary Record 91, 481–2.CrossRefGoogle ScholarPubMed
Gallie, G. J. & Sewell, M. M. H. (1974). The serological response of calves infected neonatally with T. saginata (Cysticercus bovis). Tropical Animal Health and Production 6, 173–7.CrossRefGoogle Scholar
Gemmel, M. A. (1986). A critical approach to the concepts and eradication of echinococcosis/hydatidosis and taeniasis/cysticercosis. Proceedings of the Sixth International Congress of Parasitology: Quo Vadit? (ed. Howell, M. J.) pp. 462–472. The Australian Academy of Science.Google Scholar
Gemmel, M. A., Matyas, Z., Pawlowski, Z. & Soulsby, E. J. L. (1983). Guidelines for surveillance prevention and control of Taeniasis/Cysticercosis. World Health Organization VPH/83/49.Google Scholar
Harrison, L. J. S., Joshua, G. W. P., Wright, S. H. & Parkhouse, R. M. E. (1989). Specific detection of surface/secreted glycoproteins of viable cysticerci in Taenia saginata cysticercosis. Parasite Immunology 11, 351–70.CrossRefGoogle ScholarPubMed
Harrison, L. J. S. & Parkhouse, R. M. E. (1985). Antigens of taeniid cestodes in protection, diagnosis and escape. Current Topics in Microbiology and Immunology 120, 159–72.Google ScholarPubMed
Harrison, L. J. S., Parkhouse, R. M. E. & Sewell, M. M. H. (1984). Variation in ‘target’ antigens between appropriate and inappropriate hosts of Taenia saginata metacestodes. Parasitology 88, 319–32.CrossRefGoogle ScholarPubMed
Joshua, G. W. P., Harrison, L. J. S. & Sewell, M. M. H. (1988). Excreted/secreted products of developing Taenia saginata metacestodes. Parasitology 97, 477–87.CrossRefGoogle ScholarPubMed
Joshua, G. W. P., Harrison, L. J. S. & Sewell, M. M. H. (1989). Developmental changes in proteins and glycoproteins revealed by direct radio-iodination of viable Taenia saginata larvae. Parasitology 99, 265–74.CrossRefGoogle ScholarPubMed
Kamango-Sollo, E. I. P., Lindquist, K. J. & Gathuma, J. M. (1985). Bovine serum components in Taenia saginata cysticerci (C. bovis). Bulletin of Animal Health and Production in Africa 33, 1–3.Google Scholar
Larralde, C., Laclette, J. P., Owen, C. S., Madazo, I., Sandoval, M., Bojalil, R., Sciutto, E., Contreras, L., Arzate, J., Diaz, M. L., Govezensky, T., Montoya, R. M. & Goodsaid, F. (1986). Reliable serology of Taenia solium cysticercosis with antigens from cyst vesicular fluid: ELISA and haemagglutination tests. American Journal of Tropical Medicine and Hygiene 35, 965–73.CrossRefGoogle Scholar
Machnicka, B. & Grzybowski, J. (1986). Host serum proteins in Taenia saginata metacestode fluid. Veterinary Parasitology 19, 47–54.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E. & Askonas, B. A. (1969). Immunoglobulin M biosynthesis — intracellular accumulation of 7 s subunits. The Biochemical Journal 115, 163–9.CrossRefGoogle Scholar
Parkhouse, R. M. E. & Harrison, L. J. S. (1987). Cyst fluid and surface associated glycoprotein antigens of Taenia sp. metacestodes. Parasite Immunology 9, 263–8.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E. & Harrison, L. J. S.. (1989). Antigens of parasitic helminths in diagnosis, protection and pathology. A Symposium of the British Society for Parasitology and the Linnean Society. Research Developments in the study of Parasitic Infections (ed. Anderson, R. M., Facer, C. A. & Rollinson, D). Parasitology 99, S5–S19.CrossRefGoogle Scholar
Rogers, A. W. (1970). Practical Autoradiography. Amersham International plc. review No. 20.Google Scholar
Slais, J. (1970). The morphology and pathogenicity of the bladder worms Cysticercus cellulosae and Cysticercus bovis. Pubs. Academica; Prague: Publishing House of the Czechoslovak Academy of Sciences.CrossRefGoogle Scholar
Vidor, E., Piens, M. A. & Garin, , (1987). Host serum protein levels in cysts of human hydatidosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 669–71.CrossRefGoogle ScholarPubMed