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Presence of Trypanosoma cruzi antigen on the surface of both infected and uninfected cells in tissue culture

Published online by Cambridge University Press:  06 April 2009

I. A. Abrahamsohn  and
J. K. Kloetzel
I. A. Abrahamsohn
Affiliation:
Instituto de Ciências Biomédicas da Universidade de São Paulo, Departamento de Medicina Preventiva, Faculdad de Medicina da Universidade de São Paulo and Instituto de Medicina Tropical de São Paulo, Av. Dr Enéas de Carvalho Aguiar 470, Caixa Postal 2921, São Paulo, Brasil
J. K. Kloetzel
Affiliation:
Instituto de Ciências Biomédicas da Universidade de São Paulo, Departamento de Medicina Preventiva, Faculdad de Medicina da Universidade de São Paulo and Instituto de Medicina Tropical de São Paulo, Av. Dr Enéas de Carvalho Aguiar 470, Caixa Postal 2921, São Paulo, Brasil
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Summary

LLC-MK2 cell monolayers infected with Trypanosoma cruzi were shown by immunofluorescence to present parasite antigens on the surface of both parasitized and non-parasitized cells after completion of the first intracellular cycle and rupture of infected cells. The cell-culture supernatant fluid at this stage, as well as the supernatant fluid of parasites left overnight in culture medium were concentrated and contained antigen capable of binding to uninfected cell monolayers. The origin of this antigen, as well as its eventual role in the pathogenesis of Chagas' disease, are discussed.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 1 , February 1980 , pp. 147 - 152
Copyright
Copyright © Cambridge University Press 1980

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References

Allison, A. C. (1972). Immunity against viruses. Scientific Basis of Medicine, Annual Reviews, pp. 49–73.Google ScholarPubMed
Araujo, F. G. (1976). Antígenos circulantes em camundongos experimentalmente infectados com o Trypanosoma cruzi. Revista do Instituto de Medicina Tropical de São Paulo 18, 433–9.Google Scholar
Baker, J. R. & Green, S. M. (1977). Trypanosomal antigen on macrophages cultivated in vitro with Trypanosoma (Schizotrypanum) dionisii. Annals of Tropical Medicine and Parasitology 71, 141–5.CrossRefGoogle ScholarPubMed
Brown, K. N., Brown, I. N., Trigg, P. I., Phillips, R. S. & Hills, L. A. (1970). Immunity to malaria. II. Serological response of monkeys sensitized by drug-suppressed infection or by dead parasitized cells in Freund's complete adjuvant. Experimental Parasitology 28, 318–38.CrossRefGoogle ScholarPubMed
Coleman, R. M., Rencricca, N. J., Stout, J. P., Brissette, W. H. & Smith, D. M. (1975). Splenic mediated erythrocyte cytotoxicity in malaria. Immunology 29, 49–54.Google ScholarPubMed
Dzbenski, T. H. (1974). Exoantigens of Trypanosoma cruzi in vivo. Tropenmedizin und Parasitenkunde 25, 485–91.Google ScholarPubMed
Farah, F. S., Samra, S. A. & Newayri-Salti, N. (1975). The role of the macrophage in cutaneous leishmaniasis. Immunology 29, 755–64.Google ScholarPubMed
Franco, M. F. de (1972). Cardite experimental do cobaio pela cepa Y do Trypanosoma cruzi. Correlação entre a histopatolo gia e a presença de antígenos parasitários identificados por imunofluorescência indireta. Thesis. Botucatu, Brazil.Google Scholar
Glauert, A. M. (1974). Fixation, dehydration and embedding of biological specimens. In Practical Methods in Electron Microscopy (ed. Glauert, A. M.), vol. 3, pp. 45–6, North Holland, American Elsevier.Google Scholar
Kilejian, A., Abati, A. & Trager, W. (1977). Plasmodium falciparum and Plasmodium coatneyi. Immunogenicity of ‘knob-like protrusions’ on infected erythrocyte membranes. Experimental Parasitology 42, 157–64.CrossRefGoogle ScholarPubMed
Kuhn, R. E. & Murnane, J. E. (1977). Trypanosoma cruzi: immune destruction of parasitized mouse fibroblasts in vitro. Experimental Parasitology 41, 66–73.CrossRefGoogle ScholarPubMed
Margarino Torres, C. B. (1929). Patogenia de la miocarditis cronica en la ‘enfermedad de Chagas’. Quinta Reunión de la Sociedad Argentina de Patologia Regional de Norte 2, 902–16.Google Scholar
Ribeiro dos Santos, R. & Márquez, J. O. (1977). Mecanismos imunolóogicos comprometidos en la destrucción neuronal que ocurre en la enfermedad de Chagas. Revista Neurologica Argentina 3, 439–42.Google Scholar
Santos-Buch, C. A. & Teixeira, A. R. L. (1974). The immunology of experimental Chagas' disease. III. Rejection of allogeneic heart cells in vitro. Journal of Experimental Medicine 140, 38–53.CrossRefGoogle Scholar
Siqueira, A., Ferrioli Filho, F. & Carvalheiro, J. da R. (1966). Um antígeno solúvel presente no soro de ratos infectados com Trypanosoma cruzi. Revista do Instituto de Medicina Tropical de São Paulo 8, 148.Google Scholar