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The Preparation of Gradocol Membranes and their Application in the Study of Plant Viruses

Published online by Cambridge University Press:  06 April 2009

Kenneth M. Smith  and
J. P. Doncaster
Kenneth M. Smith
Affiliation:
Potato Virus Research Station, School of Agriculture and Molteno Institute, Cambridge
J. P. Doncaster
Affiliation:
Potato Virus Research Station, School of Agriculture and Molteno Institute, Cambridge
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Extract

An account is given of the preparation of Elford's gradocol membranes and their application in the study of plant viruses. The technique is discussed step by step and the chief sources of error are indicated. It is shown that plant virus suspensions clog the membranes, and suggestions are made for the preparation of those suspensions for filtration. The effect of virus content, purification and pH of virus fluids upon their filtration is discussed.

Type
Research Article
Information
Parasitology , Volume 27 , Issue 4 , October 1935 , pp. 523 - 542
Copyright
Copyright © Cambridge University Press 1935

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References

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