Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-23T02:08:07.080Z Has data issue: false hasContentIssue false
  • English
  • Français

Population dynamics of Theileria sergenti in persistently infected cattle and vector ticks analysed by a polymerase chain reaction

Published online by Cambridge University Press:  06 April 2009

S. Kubota ,
C. Sugimoto  and
M. Onuma
S. Kubota
Affiliation:
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
C. Sugimoto
Affiliation:
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
M. Onuma*
Affiliation:
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
*
* Corresponding author.
Get access Rights & Permissions [Opens in a new window]

Summary

Theileria sergenti Shintoku stock consists of 2 parasite populations bearing 2 atlelic forms of p33/32, an immunodominant piroplasm surface protein. Parasite population changes during parasite passages among cattle and tick vectors, and during persistent infection in individual calves were analysed by using allele-specific polymerase chain reaction (PCR). The parasite DNAs were prepared from piroplasms from calves which had been infected with Shintoku stock by inoculation of sporozoite stabilates or parasitized erythrocytes, and from sporozoite stabilates which had been prepared from Shintoku stock-infected ticks. Changes in a dominant parasite population were demonstrated during transmission from calves to vector ticks and from infected ticks to calves. Parasite population changes were also apparent during persistent infection in cattle over several months, and this change is thought to occur under host immune pressure. The results of this study indicate that expression of diverse forms of p33/32 may play a role in parasite persistence within mammalian hosts and its transmission from tick vector.

Keywords

antigenic diversityApicomplexapiroplasmaPCRTheileria sergentitick.
Type
Research Article
Information
Parasitology , Volume 112 , Issue 5 , May 1996 , pp. 437 - 442
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bouharoun-Tayoun, H., Oeuvray, C., Lunel, F. & Druilhe, P. (1995). Mechanisms underlying the monocyte-mediated antibody-dependent killing of Plasmodium falciparum asexual blood stages. Journal of Experimental Medicine 182, 409–18.CrossRefGoogle ScholarPubMed
Cunningham, M. P., Brown, C. G. D., Burridge, M. J. & Purnell, R. E. (1973). Cryopreservation of ineffective particles of Theileria parva. International Journal for Parasitology 3, 583–7.CrossRefGoogle Scholar
Fujisaki, K., Ito, Y., Kamio, T. & Kitaoka, S. (1985). The presence of Theileria sergenti in Haemaphysalis longicornis over wintering in pasture in Japan. Annals of Tropical Medicine and Parasitology 79, 519–24.CrossRefGoogle Scholar
Fujisaki, K., Kamio, T., Kawazu, S., Shimizu, S. & Shimura, K. (1993). Theileria sergenti: experimental transmission by the long-nosed cattle louse, Linognathus vituli. Annals of Tropical Medicine and Parasitology 87, 217–18.CrossRefGoogle ScholarPubMed
Kawazu, S., Sugimoto, C., Kamio, T. & Fujisaki, K. (1992). Analysis of the genes encoding immunodominant piroplasm surface proteins of Theileria sergenti and Theileria buffeli by nucleotide sequencing and polymerase chain reaction. Molecular and Biochemical Parasitology 56, 169–76.CrossRefGoogle ScholarPubMed
Kimura, M., Kaneko, O., Inoue, A., Ishii, A. & Tanabe, K. (1995). Amplification by polymerase chain reaction of Plasmodium falciparum DNA from Giemsa-stained thin blood smears. Molecular and Biochemical Parasitology 70, 193–7.CrossRefGoogle ScholarPubMed
Kubota, s., Sugimoto, c. & Onuma, M. (1995). A genetic analysis of mixed population in Theileria sergenti stocks and isolates using allele-specific polymerase chain reaction. Journal of Veterinary Medical Science 57, 279–82.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Lennard, P. (1990). Image analysis for all. Nature, London 347, 103–4.CrossRefGoogle ScholarPubMed
Matsuba, T., Kawakami, Y., Iwai, H. & Onuma, M. (1992). Genomic analysis of Theileria sergenti stocks in Japan with DNA probes. Veterinary Parasitology 41, 35–43.CrossRefGoogle ScholarPubMed
Matsuba, T., Sugimoto, C., Onoe, S., Kawakami, Y., Iwai, H. & Onuma, M. (1993 a). Changes in the hybridization patterns of populations of Theileria sergenti during infection. Veterinary Parasitology 47, 215–23.CrossRefGoogle ScholarPubMed
Matsuba, T., Kubota, H., Tanaka, M., Hattori, M., Murata, M., Sugimoto, C. & Onuma, M. (1993 b). Analysis of mixed parasite populations of Theileria sergenti using cDNA probes encoding a major piroplasm surface protein. Parasitology 107, 369–77.CrossRefGoogle ScholarPubMed
Mehlhorn, H. & Schein, E. (1984). The piroplasms: Life cycle and sexual stages. Advances in Parasitology 23, 37–103.CrossRefGoogle ScholarPubMed
Minami, T., Fujinaga, T., Furuya, K. & Ishihara, T. (1980). Clinico-hematologic and serologic comparisons of Japanese and Russian strains of Theileria sergenti. National Institute of Animal Health Quarterly (Japan) 20, 44–52.Google ScholarPubMed
Ohgitani, T., Okabe, T. & Sasaki, N. (1987). Antigenic properties of Theileria sergenti in ELISA serodiagnosis. Japanese Journal of Veterinary Science 49, 531–4.Google ScholarPubMed
Shiels, B. R., D'Oliveira, C., McKellar, S., Ben-Miled, L., Kawazu, S. & Hide, G. (1995). Selection of diversity at putative glycosylation sites in the immunodominant merozoite/piroplasm surface antigen of Theileria parasites. Molecular and Biochemical Parasitology 72, 149–62.CrossRefGoogle ScholarPubMed
Shirakata, S., Onuma, M., Kirisawa, R., Takahashi, K. & Kawakami, Y. (1989). Localization of surface antigens on Theileria sergenti merozoite by monoclonal antibodies. Japanese Journal of Veterinary Science 51, 831–3.Google ScholarPubMed
Sugimoto, C., Sato, M., Kawazu, S., Kamio, T. & Fujisaki, K. (1991). Purification of merozoites of Theileria sergenti from infected bovine erythrocytes. Parasitology Research 77, 129–31.CrossRefGoogle ScholarPubMed
Tanaka, M., Onoe, S., Matsuba, T., Katayama, S., Yamanaka, M., Yonemichi, H., Hiramatsu, K., Beak, B. K., Sugimoto, c. & Onuma, M. (1993). Detection of Theileria sergenti infection in cattle by polymerase chain reaction amplification of parasite-specific DNA. Journal of Clinical Microbiology 31, 2565–9.CrossRefGoogle ScholarPubMed
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Zhuang, W. Z., Kubota, S., Sugimoto, C. & Onuma, M. (1993). Characterization of epitopes on a 32 kDa merozoite surface protein of Theileria sergenti. Parasite Immunology 15, 113–19.CrossRefGoogle ScholarPubMed
Zhuang, W. Z., Sugimoto, C., Matsuba, T., Niinuma, S., Murata, M. & Onuma, M. (1994). Analysis of antigenic and genetic diversities of Theileria sergenti piroplasm surface proteins. Journal of Veterinary Medical Science 56, 469–73.CrossRefGoogle ScholarPubMed
Zhuang, W. Z., Sugimoto, C., Kubota, S. & Onuma, M. (1995). Antigenic alteration in major piroplasm surface proteins of Theileria sergenti during infection. Veterinary Parasitology 60, 191–8.CrossRefGoogle ScholarPubMed