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Polar filament discharge of Myxobolus cerebralis actinospores is triggered by combined non-specific mechanical and chemical cues

Published online by Cambridge University Press:  22 July 2005

D. M. KALLERT ,
M. EL-MATBOULI  and
W. HAAS
D. M. KALLERT
Affiliation:
Institute for Zoology I, University Erlangen, Staudtstrasse 5 D-91058, Erlangen, Germany
M. EL-MATBOULI
Affiliation:
Institute of Zoology, Fish Biology and Fish Diseases, University of Munich, Kaulbachstraße 37, D-80539 Munich, Germany
W. HAAS
Affiliation:
Institute for Zoology I, University Erlangen, Staudtstrasse 5 D-91058, Erlangen, Germany
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Abstract

This study presents initial evidence for the requirement of both chemical and mechanical stimuli to discharge polar capsules of Myxobolus cerebralis actinospores, the causative agent of salmonid whirling disease. The obligate need for combined discharge triggers was concluded from data obtained in a before/after experimental set-up carried out with individual locally immobilized actinospores. Homogenized rainbow trout mucus as chemostimulus and tangency of the apical region of the spores to achieve mechanical stimulation were applied subsequently. The actinospores showed discharged polar filaments exclusively when mucus substrate application was followed by touching the polar capsule-bearing region, but not when either stimulus was offered solely to the same individuals. We measured filament discharge rates to mucus preparations in a microscopic assay using supplementary vibration stimuli to ensure mechanical excitation. The actinospores responded similarly to different frequencies, which suggested a touch-sensitive recognition mechanism. Discharge specificity for salmonid mucus could not be confirmed, as mucus of common carp and bream could trigger similar filament expulsion rates. To a lesser extent homogenized frog epidermis and bovine submaxillary mucin could also stimulate the attachment reaction. In contrast, mucus of a pulmonate freshwater snail elicited no response.

Keywords

MyxozoaMyxobolus cerebralispolar capsule dischargechemo-perceptionhost specificitymucus
Type
Research Article
Information
Parasitology , Volume 131 , Issue 5 , November 2005 , pp. 609 - 616
Copyright
© 2005 Cambridge University Press

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