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Plasmodium berghei and Plasmodium chabaudi chabaudi: development of simple in vitro erythrocyte invasion assays

Published online by Cambridge University Press:  06 April 2009

J. McNally ,
S. M. O'donovan  and
J. P. Dalton
J. McNally
Affiliation:
School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland
S. M. O'donovan
Affiliation:
School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland
J. P. Dalton
Affiliation:
School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland
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Summary

Erythrocyte invasion assays are described for two species of rodent malaria, namely Plasmodium berghei and P. c. chabaudi. These invasion assays are simple, are carried out using a candle jar and allow a number of assays to be performed simultaneously. Our results demonstrate that both rodent malaria species show an in vitro preference for reticulocytes although the preference of P. c. chabaudi for these cells is not as marked as that of P. berghei. The details of our invasion assays and our results obtained are discussed.

Keywords

Plasmodium bergheiPlasmodium chabaudi chabaudimalariarodentreticulocyteserythrocytesinvasion assays
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 355 - 362
Copyright
Copyright © Cambridge University Press 1992

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