Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-26T16:58:57.507Z Has data issue: false hasContentIssue false
  • English
  • Français

Plasmodium berghei and Plasmodium chabaudi chabaudi: development of simple in vitro erythrocyte invasion assays

Published online by Cambridge University Press:  06 April 2009

J. McNally ,
S. M. O'donovan  and
J. P. Dalton
J. McNally
Affiliation:
School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland
S. M. O'donovan
Affiliation:
School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland
J. P. Dalton
Affiliation:
School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland
Get access Rights & Permissions [Opens in a new window]

Summary

Erythrocyte invasion assays are described for two species of rodent malaria, namely Plasmodium berghei and P. c. chabaudi. These invasion assays are simple, are carried out using a candle jar and allow a number of assays to be performed simultaneously. Our results demonstrate that both rodent malaria species show an in vitro preference for reticulocytes although the preference of P. c. chabaudi for these cells is not as marked as that of P. berghei. The details of our invasion assays and our results obtained are discussed.

Keywords

Plasmodium bergheiPlasmodium chabaudi chabaudimalariarodentreticulocyteserythrocytesinvasion assays
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 355 - 362
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Carter, R. & Walker, D. (1975). New observations on the malaria parasites of the Central African Republic, Plasmodium vinckei petteri subsp. nov and P. chabaudi Landau (1965). Annals of Tropical Medicine and Parasitology 69, 187–96.CrossRefGoogle ScholarPubMed
Dalton, J. P., Hudson, D., Adams, J. H. & Miller, L. H. (1991). Blocking of the receptor-mediated invasion of erythrocytes by Plasmodium knowlesi malaria with sulfated polysaccharides and glycosaminoglycans. European Journal of Biochemistry 195, 789–94.CrossRefGoogle ScholarPubMed
Gronowicz, G., Swift, H. & Steck, T. L. (1984). Maturation of the reticulocyte in vitro. Journal of Cell Science 71, 177–97.CrossRefGoogle ScholarPubMed
Hadley, T. J., Klotz, F. W., Pasvol, G., Haynes, J. D., McGinniss, M. H., Okubo, Y. & Miller, L. H. (1987). Falciparum malaria parasites invade erythrocytes that lack glycophorin A and B (Mk Mk). Journal of Clinical Investigations 80, 1190–3.Google Scholar
Hadley, T. J., McGinniss, M. H., Klotz, F. W. & Miller, L. H. (1986). Blood group antigens and invasion of erythrocytes by malaria parasites. In Red Cell Antigens and Antibodies (ed. Garratty, G.), pp. 17–33. Arlington, VA, USA. American Association of Blood Banks.Google Scholar
Haynes, J. D., Dalton, J. P., Klotz, F. W., McGinniss, M. H., Hadley, T. J., Hudson, D. E. & Miller, L. H. (1988). Receptor-like specificity of a Plasmodium knowlesi malaria protein that binds to Duffy antigen ligands on erythrocytes. Journal of Experimental Medicine 167, 1873–81.Google Scholar
Hudson, D. E., Wellems, T. E. & Miller, L. H. (1988). Molecular basis for mutation in a surface protein expressed by malaria parasites. Journal of Molecular Biology 203, 707–14.Google Scholar
Janse, C. J., Boorsma, E. G., Ramesar, J., Grobbee, M. J. & Mons, B. (1989). Host cell specificity and schizogony of Plasmodium berghei under different in vitro conditions. International Journal for Parasitology 19, 509–14.CrossRefGoogle ScholarPubMed
Janse, C. J., Mons, B., Croon, J. J. A. B. & Van Der Kaay, H. J. (1984). Long-term in vitro cultures of Plasmodium berghei and preliminary observations on gametocytogenesis. International Journal for Parasitology 14, 317–20.Google Scholar
Jarra, W. & Brown, K. N. (1989). Invasion of mature and immature erythrocytes of CBA/Ca mice by a cloned line of Plasmodium chabaudi chabaudi. Parasitology 99, 157–63.CrossRefGoogle ScholarPubMed
Mclean, S. A., Macdougall, L. & Phillips, R. S. (1986). An indirect fluorescent antibody test which recognizes antigenic variants of Plasmodium chabaudi. IRCS Medical Science 14, 896–7.Google Scholar
Miller, L. H. & Carter, R. (1976). A review. Innate resistance in malaria. Experimental Parasitology 40, 132–46.Google Scholar
Miller, L. H., Mason, S. J., Clyde, D. F., Mcginniss, M. H. & Rothman, I. K. (1975). Erythrocyte receptors for (Plasmodium knowlesi) malaria: Duffy blood group determinants. Science 189, 561–3.CrossRefGoogle ScholarPubMed
Mons, B., Janse, C. J., Boorsma, E. G. & Van Der, Kaay, H., J. (1985). Synchronized erythrocytic schizogony and gametocytogenesis of Plasmodium berghei in vivo and in vitro. Parasitology 91, 423–30.Google Scholar
Ramakrishnan, S. P. & Prakash, S. (1950). Studies on Plasmodium berghei n. sp. Vinke and Lips 1948. 11. Morphology, periodicity and pathogeneity in blood induced infection in mice, rats and garden squirrels. Indian Journal of Malariology 4, 369–75.Google Scholar
Rapoport, S. M. (1986). The Reticulocyte. Boca Raton, Florida: CRC Press.Google Scholar
Suhrbier, A., Janse, C., Mons, B., Fleck, S. L., Nicholas, J., Davies, C. S. & Sinden, R. E. (1987). The complete development in vitro of the vertebrate phase of the mammalian malaria parasite Plasmodium berghei. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 907–9.Google Scholar
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–5.Google Scholar
Wunderlich, F., Schillinger, G. & Helwig, M. (1985). Fractionation of Plasmodium chabaudi-infected erythrocytes into parasites and ghosts. Zeitschrift für Parasitenkunde 71, 545–51.Google Scholar
Yoeli, M., Hargreaves, B., Carter, R. & Walliker, D. (1975). Sudden increase in virulence in a strain of Plasmodium berghei yoelii. Annals of Tropical Medicine and Parasitology 69, 173–8.Google Scholar