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Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta as a potential biomarker for Opisthorchis viverrini infection and cholangiocarcinoma

Published online by Cambridge University Press:  05 October 2021

Sattrachai Prasopdee ,
Yodying Yingchutrakul ,
Sittiruk Roytrakul ,
Montinee Pholhelm ,
Teva Phanaksri ,
Anthicha Kunjantarachot ,
Jutharat Kulsantiwong ,
Kritiya Butthongkomvong ,
Smarn Tesana  and
Thanakrit Sathavornmanee
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Sattrachai Prasopdee
Affiliation:
Thammasat University Research Unit in Opisthorchiasis, Cholangiocarcinoma, and Neglected Parasitic Diseases, Thammasat University, Pathum Thani, 12120, Thailand Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand
Yodying Yingchutrakul
Affiliation:
Proteomics Research Team, National Omics Center, NSTDA, Pathum Thani, 12120, Thailand
Sittiruk Roytrakul
Affiliation:
Proteomics Research Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), NSTDA, Pathum Thani, 12120, Thailand
Montinee Pholhelm
Affiliation:
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand
Teva Phanaksri
Affiliation:
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand
Anthicha Kunjantarachot
Affiliation:
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand
Jutharat Kulsantiwong
Affiliation:
Faculty of Science, Udon Thani Rajabhat University, Udon Thani, 41000, Thailand
Kritiya Butthongkomvong
Affiliation:
Medical Oncology Unit, Udonthani Cancer Hospital, Udon Thani, 41330, Thailand
Smarn Tesana
Affiliation:
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand
Thanakrit Sathavornmanee
Affiliation:
Chonburi hospital, Ministry of Public Health, Chonburi, 20000, Thailand
Veerachai Thitapakorn*
Affiliation:
Thammasat University Research Unit in Opisthorchiasis, Cholangiocarcinoma, and Neglected Parasitic Diseases, Thammasat University, Pathum Thani, 12120, Thailand Chulabhorn International College of Medicine, Thammasat University, Pathum Thani, 12120, Thailand
*
Author for correspondence: Veerachai Thitapakorn, E-mail: [email protected]
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Abstract

The human liver fluke Opisthorchis viverrini (Ov), the primary risk factor for cholangiocarcinoma (CHCA), is a parasite endemic to southeast Asian countries. With no effective treatments for CHCA currently available, early diagnosis and treatment of Ov infection remains the only practical method for the prevention of CHCA. In this study, plasma phosphoproteomes of patients in the non-Ov infection, non-cholangiocarcinoma subject group (non-OVCCA), the asymptomatic Ov infected group (OV), and the CHCA group (CCA), were investigated to identify potential biomarkers for Ov infection and CHCA. The AKT signalling pathway was found to be up-regulated. Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB), an upstream signalling molecule, was selected as a potential biomarker and evaluated using indirect enzyme-linked immunosorbent assay (ELISA). Results demonstrated evidence that levels of PIK3CB in both the OV group and CCA group was statistically different compared to the non-OVCCA group (P < 0.01). However, the levels of PIK3CB between the OV group and the CCA group were found not to be statistically different. Sensitivity and specificity for OV using OD450 cut-off at >1.570 was 76 and 72%, respectively. For CCA, sensitivity and specificity using OD450 cut-off at >1.398 was 68 and 76%, respectively. Application of indirect ELISA detecting plasma PIK3CB will be of great benefit for screening of opisthorchiasis and CHCA.

Keywords

BiomarkercholangiocarcinomadiagnosisOpisthorchis viverriniPhosphoproteomePIK3CB
Type
Research Article
Information
Parasitology , Volume 149 , Issue 2 , February 2022 , pp. 171 - 180
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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