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Penetration of mammalian skin by the infective larva of Nippostrongylus brasiliensis

Published online by Cambridge University Press:  06 April 2009

D. L. Lee
D. L. Lee
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds, LS2 9JT
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Extract

The behaviour of the third-stage larvae of Nippostrongylus brasiliensis has been studied on rat, mouse and human skin. Locomotion in thin films of moisture is by two-dimensional, undulatory propulsion on skin, and by three-dimensional, undulatory propulsion on hairs. In drops of water or in thick films of moisture locomotion is less efficient than in thin films. It is suggested that the thin film of moisture around larvae on the skin may become coated with a thin monolayer of lipid which could reduce evaporation from the surface of the nematode and from the film of moisture, thus extending the period before desiccation begins. Studies with the electron microscope have shown that the larvae move horizontally into the stratum corneum and later penetrate the epidermis and dermis by separating the constituent cells of these tissues. In the dermis there is dissolution of collagen around the larvae. It is suggested that this separation of cells and dissolution of collagen need not be brought about by enzyme action, as similar effects can be brought about by changes in pH or ionic composition of the bathing medium.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 3 , December 1972 , pp. 499 - 505
Copyright
Copyright © Cambridge University Press 1972

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References

Gharib, H. M. (1955). Observations on skin penetration by the infective larvae of Nippostronqylus brasiliensis. Journal of Helminihology 24, 33–6.CrossRefGoogle Scholar
Gordon, A. B., Miller, L. R. & Bensch, K. G. (1963). Fixation of tissue culture cells for ultrastructural cytochemistry. Experimental Cell Research 31, 440–3.CrossRefGoogle ScholarPubMed
Keeling, J. E. D. (1960). The effects of ultra-violet radiation on Nippostrongylus muris. I. Irradiation of infective larvae; lethal and sub-lethal effects. Annals of Tropical Medicine and Parasitology 54, 182–91.CrossRefGoogle Scholar
Lee, D. L. (1966). The structure and composition of the helminth cuticle. Advances in Parasitology 4, 187–254.Google Scholar
Lee, D. L. (1970). The fine structure of the excretory system in adult Nippostrongylus brasiliensis (Nematoda) and a suggested function for the ‘excretory glands’. Tissue and Cell 2, 225–31.CrossRefGoogle Scholar
Lee, D. L. (1972). The structure of the helminth cuticle. Advances in Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Lewert, B. M. & Lee, C. L. (1954) Studies on the passage of helminth larvae through host tissues. I. Histochemical studies on extracellular changes caused by penetrating larvae. II. Enzymatic activity of larvae in vitro and in vivo. Journal of Infectious Diseases 95, 13–51.Google Scholar
Pearse, A. G. E., (1960). Histochemistry: Theoretical and Applied, 2nd ed.London: Churchill.Google Scholar
Rosenbluth, J. (1965). Ultrastructural organization of obliquely striated muscle fibres in Ascaris lumbricoides. Journal of Cell Biology 25, 494–515.Google ScholarPubMed
Stirewalt, M. A. (1963). Cercaria vs. Schistosomule (Schistosoma mansoni): absence of the pericercarial envelope in vivo and the early physiological and histological metamorphosis of the parasite. Experimental Parasitology 13, 395–406.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. (1966). Skin penetration mechanisms of helminths. In Biology of Parasites (ed. Soulsby, E. J. C.), pp. 41–59. New York: Academic Press.Google Scholar
Taliaferro, W. H. & Sarles, M. P. (1939). The cellular reactions in the skin, lungs and intestine of normal and immune rats after infection with Nippostrongylus muris. Journal of Infectious Diseases 64, 157–92.CrossRefGoogle Scholar
Wallace, H. R., (1963). The Biology of Nematodes. London: Arnold.Google Scholar