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Parasites infecting the cultured oyster Crassostrea gasar (Adanson, 1757) in Northeast Brazil

Published online by Cambridge University Press:  02 January 2015

FERNANDO RAMOS QUEIROGA ,
ROGÉRIO TUBINO VIANNA ,
CAIRÉ BARRETO VIEIRA ,
NATANAEL DANTAS FARIAS  and
PATRICIA MIRELLA DA SILVA
FERNANDO RAMOS QUEIROGA
Affiliation:
Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba – Campus I, 58051-900, João Pessoa, PB, Brazil
ROGÉRIO TUBINO VIANNA
Affiliation:
Universidade Federal do Rio Grande, CEP 96203-900, Rio Grande, RS, Brazil
CAIRÉ BARRETO VIEIRA
Affiliation:
Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba – Campus I, 58051-900, João Pessoa, PB, Brazil
NATANAEL DANTAS FARIAS
Affiliation:
Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba – Campus I, 58051-900, João Pessoa, PB, Brazil
PATRICIA MIRELLA DA SILVA*
Affiliation:
Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba – Campus I, 58051-900, João Pessoa, PB, Brazil
*
* Corresponding author. Laboratório de Imunologia e Patologia de Invertebrados, Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba – Campus I, 58051-900, João Pessoa, PB, Brazil. E-mail: [email protected]
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Summary

The oyster Crassostrea gasar is a species widely used as food and a source of income for the local population of the estuaries of Northeast Brazil. Perkinsus marinus and Perkinsus olseni are deleterious parasites for oyster farming and were recently detected in Brazil. In this study, a histopathologic survey of the oyster C. gasar cultured in the estuary of the River Mamanguape (Paraíba State) was performed. Adult oysters were collected in December 2011 and March, May, August and October 2012 and processed for histology and Perkinsus sp. identification by molecular analyses. Histopathological analysis revealed the presence of parasitic organisms including viral gametocytic hypertrophy, prokaryote-like colonies, protozoans (Perkinsus sp. and Nematopsis sp.) and metazoans (Tylocephalum sp. and cestodes). Other commensal organisms were also detected (the protozoan Ancistrocoma sp. and the turbellarian Urastoma sp.). The protozoan parasite Perkinsus sp. had the highest overall prevalence among the symbiotic organisms studied (48·9%), followed by Nematopsis sp. (36·3%). The other organisms were only sporadically observed. Only the protozoan Perkinsus sp. caused alterations in the oysters’ infected organs. Molecular analyses confirmed the presence of P. marinus, P. olseni and Perkinsus beihaiensis infecting the oyster C. gasar. This is the first report of P. beihaiensis in this oyster species.

Keywords

Crassostrea gasarPerkinsus sp.histopathologyPCR–RFLPrDNA
Type
Research Article
Information
Parasitology , Volume 142 , Issue 6 , May 2015 , pp. 756 - 766
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Copyright
Copyright © Cambridge University Press 2015 

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