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NO nerves in a tapeworm. NADPH-diaphorase histochemistry in adult Hymenolepis diminuta

Published online by Cambridge University Press:  06 April 2009

M. K. S. Gustafsson ,
A. M. Lindholm ,
N. B. Terenina  and
M. Reuter
M. K. S. Gustafsson*
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
A. M. Lindholm
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
N. B. Terenina
Affiliation:
Institute of Parasitology of the Russian Academy of Sciences, Lenin Avenue 33, 117071 Moscow, Russia
M. Reuter
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
*
* Corresponding author: Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland. Tel: + 358 21 2654603. Fax: + 358 21 2654748. E-mail: [email protected].
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Summary

The free radical nitric oxide (NO), which is synthesized by nitric oxide synthase (NOS), has recently been discovered to function as a neuronal messenger. The presence of NOS was detected in the nervous system of adult Hymenolepis diminuta with NADPH-diaphorase (NADPH-d) histochemistry. The NADPH-d histochemical reaction is regarded as a selective marker for NOS in neuronal tissue. NADPH-d staining was observed in nerve fibres in the main and minor nerve cords and the transverse ring commissures, and in cell bodies in the brain commissure, along the main nerve cords, in the suckers and the rostellar sac. NADPH-d staining was also observed in the wall of the internal seminal vesicle and the genital atrium. The pattern of NADPH-d staining was compared with that of the 5-HT immunoreactive nervous elements. The NADPH-d staining reaction and the 5-HT immunoreactivity occur in separate sets of neurons. This is the first time the NADPH-d reaction has been demonstrated in the nervous system of a flatworm, indicating that NOS is present and that NO can be produced at this level of evolution.

Keywords

Hymenolepis diminutanitric oxideNADPH-diaphoraseserotoninnervous system
Type
Research Article
Information
Parasitology , Volume 113 , Issue 6 , December 1996 , pp. 559 - 565
Copyright
Copyright © Cambridge University Press 1996

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