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Nitric oxide radicals in leucocytes and vaginal washes of Trichomonas vaginalis-infected symptomatic and asymptomatic women

Published online by Cambridge University Press:  25 November 2005

M. YADAV ,
M. L. DUBEY ,
I. GUPTA  and
N. MALLA
M. YADAV
Affiliation:
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
M. L. DUBEY
Affiliation:
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
I. GUPTA
Affiliation:
Department of Obstetrics and Gynaecology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
N. MALLA
Affiliation:
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
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Abstract

The clinical spectrum of Trichomonas vaginalis infection varies from asymptomatic to mild, moderate or severe vaginitis. Nitric oxide and other reactive nitrogen radicals produced by immune effector cells are important cytotoxic and cytostatic mediators against several microorganisms including parasites. In the present study, inducible nitric oxide synthase (iNOS) and reactive nitrogen intermediates (RNI) were determined in leucocyte cultures (stimulated with T. vaginalis in vitro) and vaginal washes (VWs) of 22 symptomatic and 20 asymptomatic T. vaginalis-infected and 20 healthy women by immunoblotting and Griess method respectively. The iNOS protein was detected in leucocytes and VWs of all the symptomatic and asymptomatic women, but was not detected in any of the samples from healthy women. Mean iNOS protein band intensity was significantly higher in leucocytes as compared to VWs (P<0·001) of both symptomatic and asymptomatic women and was also higher in leucocytes of asymptomatic as compared to symptomatic women (P<0·05). Mean RNI concentration was also significantly higher in leucocytes (P<0·01) and VWs (P<0·05) of asymptomatic as compared to symptomatic women, and was also higher in samples of infected as compared to healthy women (P<0·001). These results suggest that reactive nitrogen radicals may have a role in limiting T. vaginalis infection in asymptomatic women.

Keywords

inducible nitric oxide synthasereactive nitrogen intermediatesTrichomonas vaginalistrichomoniasis
Type
Research Article
Information
Parasitology , Volume 132 , Issue 3 , March 2006 , pp. 339 - 343
Copyright
© 2005 Cambridge University Press

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