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New insights on the Golgi complex of Tritrichomonas foetus

Published online by Cambridge University Press:  18 October 2013

IVONE De ANDRADE ROSA ,
MARJOLLY BRIGIDO CARUSO ,
SILAS PESSINI RODRIGUES ,
REINALDO BARROS GERALDO ,
LUIZA WILGES KIST ,
MAURICIO REIS BOGO ,
LUIZ GONZAGA ,
ANA TEREZA R DE VASCONCELOS ,
JOSE ANDRES MORGADO-DÍAZ  and
RUSSOLINA BENEDETA ZINGALI
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IVONE De ANDRADE ROSA
Affiliation:
Universidade Santa Úrsula, Rio de Janeiro, Brazil Programa de Ciências Morfológicas – Universidade Federal do Rio de Janeiro, Brazil Instituto de Biofísica Carlos Chagas Filho – Universidade Federal do Rio de Janeiro, Brazil
MARJOLLY BRIGIDO CARUSO
Affiliation:
Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) – Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem – INBEB, Brazil
SILAS PESSINI RODRIGUES
Affiliation:
Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) – Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem – INBEB, Brazil
REINALDO BARROS GERALDO
Affiliation:
Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) – Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem – INBEB, Brazil
LUIZA WILGES KIST
Affiliation:
Laboratório de Biologia Genômica e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil
MAURICIO REIS BOGO
Affiliation:
Laboratório de Biologia Genômica e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil
LUIZ GONZAGA
Affiliation:
Laboratório Nacional de Computação Cientifica (LNCC/MCT), Petrópolis, Rio de Janeiro, Brazil
ANA TEREZA R DE VASCONCELOS
Affiliation:
Laboratório Nacional de Computação Cientifica (LNCC/MCT), Petrópolis, Rio de Janeiro, Brazil
JOSE ANDRES MORGADO-DÍAZ
Affiliation:
INCA – Grupo de Biologia Estrutural, Divisão de Biologia Celular, Instituto Nacional de Câncer, Rio de Janeiro, Brazil
RUSSOLINA BENEDETA ZINGALI
Affiliation:
Instituto de Bioquímica Medica (Unidade de Espectrometria de Massa e Proteômica) – Universidade Federal do Rio de Janeiro e Instituto Nacional de Biologia Estrutural e Bioimagem – INBEB, Brazil
MARLENE BENCHIMOL*
Affiliation:
Universidade Santa Úrsula, Rio de Janeiro, Brazil
*
* Corresponding author: Rua Jornalista Orlando Dantas 59, Botafogo, CEP 22231-010 Rio de Janeiro, RJ, Brazil. E-mail: [email protected]
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Summary

Tritrichomonas foetus is a protist that causes bovine trichomoniasis and presents a well-developed Golgi. There are very few studies concerning the Golgi in trichomonads. In this work, monoclonal antibodies were raised against Golgi of T. foetus and used as a tool on morphologic and biochemical studies of this organelle. Among the antibodies produced, one was named mAb anti-Golgi 20.3, which recognized specifically the Golgi complex by fluorescence and electron microscopy. By immunoblotting this antibody recognized two proteins with 60 and 66 kDa that were identified as putative beta-tubulin and adenosine triphosphatase, respectively. The mAb 20.3 also recognized the Golgi complex of the Trichomonas vaginalis, a human parasite. In addition, the nucleotide coding sequences of these proteins were identified and included in the T. foetus database, and the 3D structure of the proteins was predicted. In conclusion, this study indicated: (1) adenosine triphosphatase is present in the Golgi, (2) ATPase is conserved between T. foetus and T. vaginalis, (3) there is new information concerning the nucleic acid sequences and protein structures of adenosine triphosphatase and beta-tubulin from T. foetus and (4) the mAb anti-Golgi 20.3 is a good Golgi marker and can be used in future studies.

Keywords

Tritrichomonas foetusGolgi complexmonoclonal antibodiesadenosine triphosphatasemass spectrometry
Type
Research Article
Information
Parasitology , Volume 141 , Issue 2 , February 2014 , pp. 241 - 253
Copyright
Copyright © Cambridge University Press 2013 

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