Hostname: page-component-745bb68f8f-v2bm5 Total loading time: 0 Render date: 2025-01-13T11:51:12.890Z Has data issue: false hasContentIssue false
  • English
  • Français

New defined and semi-defined media for cultivation of the flagellate Trichomonas vaginalis

Published online by Cambridge University Press:  06 April 2009

David Linstead
David Linstead
Affiliation:
Department of Parasitology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
Get access Rights & Permissions [Opens in a new window]

Summary

Studies on the nutrition and physiology of Trichomonas vaginalis have been hampered by the lack of a defined synthetic medium in which to grow the organism. New synthetic media free of ill-defined supplements and serum which support good growth of T. vaginalis have been developed in this study. The first successful approach to a defined medium resulted from the modification of the tissue-culture medium CMRL 1066. Essential modifications included (1) lowering the redox potential of the medium by adding high levels of ascorbate and (2) modifying the buffer system. Subsequently, CMRL 1066 was replaced by two new media DL7 and DL8 which have greater flexibility in their formulation and permit improved serum-free growth. Serum was replaced by bovine serum albumin and cholesterol, together with either a glyceryl fatty acid ester or a defined mixture of fatty acids. Preliminary nutritional studies using the new media have indicated a requirement for saturated and unsaturated fatty acids and a sterol.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 1 , August 1981 , pp. 125 - 137
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Budilová, M. & Kulda, J. (1978). Further studies on the effect of ferric ammonium citrate on the virulence of Tritrichomonas foetus to mice. Journal of Protozoology 25, Suppl. 1, 34A.Google Scholar
Cross, G. A. M. & Manning, J. C. (1973). Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media. Parasitology 67, 315–31.Google Scholar
Eagle, H. (1959). Amino acid metabolism in mammalian cell cultures. Science 130, 432–7.CrossRefGoogle ScholarPubMed
Evans, V. J., Bryant, J. C., Kerr, H. A. & Schilling, E. L. (1964). Chemically defined media for cultivation of long-term cell strains from four mammalian species. Experimental Cell Research 36, 439–74.CrossRefGoogle ScholarPubMed
FarrisV, K. V, K. & Honigberg, B. M. (1970). Behavior and pathogenicity of Trichomonas vaginalis Donné in chick liver cell culture. Journal of Parasitology 56, 849–82.Google Scholar
Heyworth, P. G. & Gutteridge, W. E. (1978). Purine and pyrimidine metabolism in Trichomonas vaginalis. Parasitology 77, xi.Google Scholar
Heyworth, P. G., Gutteridge, W. E. & Ginger, C. D. (1979). Pyrimidine biosynthesis in Trichomonas vaginalis. Parasitology 79, xli.Google Scholar
Hill, B., McIntosh, R., Ginger, C. D., Kilsby, J. & Rogerson, G. W. (1979). The enzymes of pyrimidine biosynthesis in a range of parasite helminths and protozoa. Parasitology 79, xli.Google Scholar
Kulda, J. (1967). Effect of different species of trichomonads on monkey kidney cell cultures. Folia Parasitologia 14, 295–310.Google Scholar
Kulda, J., Honigberg, B. M., Frost, J. K. & Hollander, D. H. (1970). Pathogenicity of Trichomonas vaginalis. American Journal of Obstetrics and Gynecology 108, 908–18.Google Scholar
Lee, J. J. & Pierce, S. (1960). Hypotrichomonas acosta (Moskowitz)from reptiles. II. Physiology. Journal of Protozoology 7, 402–9.CrossRefGoogle Scholar
Linstead, D. (1979). Evolution of a serum-free medium for the cultivation of the protozoan Trichomonas vaginalis. Parasitology 79, xlii.Google Scholar
Lund, P. G. & Shorb, M. S. (1962). Steroid requirements of trichomonads. Journal of Protozoology 9, 151–4.CrossRefGoogle ScholarPubMed
Mandel, M. & Honigberg, B. M. (1957). The response of Trichomonas gallinae to cholesterol and dihydrocholesterol. Anatomical Record 128, 586.Google Scholar
Meyer, E. A. (1976). Giardia lamblia: isolation and axenic cultivation. Experimental Parasitology 27, 101–5.CrossRefGoogle Scholar
Neal, R. A., Latter, V. S. & Richards, W. H. G. (1974). Survival of Entamoeba and related amoebae at low temperature – II Viability of amoebae and cysts stored in liquid nitrogen. International Journal for Parasitology 4, 353–60.Google Scholar
Parker, R. C., LaRoy, N. C. & McCulloch, E. A. (1957). Altered cell strains in continuous culture, a general survey. Special Publications, New York Academy of Science 5, 303–13.Google Scholar
Roitman, I., Heyworth, P. G. & Gutteridge, W. E. (1978). Lipid synthesis by Trichomonas vaginalis. Annals of Tropical Medicine and Parasitology 72, 583–5.Google Scholar
Samuels, R. (1965). Growth of axenic trichomonads in a serum-free medium. Progress in Protozoology,2nd International Conference of Protozoology,Exerpta Medica Foundation, International Congress Series no. 91, page 200.Google Scholar
Shorb, M. S. (1964). The physiology of Trichomonads. In Biochemistry and Physiology of Protozoa, vol. 3 (ed. Hunter, S. H. and Lwoff, A.), pp. 383–457. New York: Academic Press.Google Scholar
Shorb, M. S. & Lund, P. G. (1958). Fatty acid and other requirements of Trichomonas gallinae. Journal of Protozoology 5, Suppl. 15.Google Scholar
Shorb, M. S. & Lund, P. G. (1959). Requirements of Trichomonads for unidentified growth factors, saturated and unsaturated fatty acids. Journal of Protozoology 6, 122–30.CrossRefGoogle Scholar