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Nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes

Published online by Cambridge University Press:  19 July 2007

G. STEPEK ,
R. H. C. CURTIS ,
B. R. KERRY ,
P. R. SHEWRY ,
S. J. CLARK ,
A. E. LOWE ,
I. R. DUCE ,
D. J. BUTTLE  and
J. M. BEHNKE
G. STEPEK
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
R. H. C. CURTIS*
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
B. R. KERRY
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
P. R. SHEWRY
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
S. J. CLARK
Affiliation:
Rothamsted Research, Harpenden, Herts AL5 2JQ, UK
A. E. LOWE
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
I. R. DUCE
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
D. J. BUTTLE
Affiliation:
Academic Unit of Molecular Medicine, University of Sheffield, Sheffield S10 2RX, UK
J. M. BEHNKE
Affiliation:
School of Biology, University of Nottingham, Nottingham NG7 2RD, UK
*
*Corresponding author: Division of Plant Pathogen Interactions, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK. Tel: 44 1582 763 133 2437. Fax: 44 1582 760 981. E-mail: [email protected]
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Summary

Cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. In this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera Meloidogyne and Globodera. All the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nematodes. The specific inhibitor of cysteine proteinases, E-64, blocked this activity completely, indicating that it was essentially mediated by cysteine proteinases. In vitro, plant cysteine proteinases are active against second-stage juveniles of M. incognita and M. javanica, and some cysteine proteinases also affect the second-stage juveniles of Globodera rostochiensis. It is not known yet whether these plant extracts will interfere with, or prevent invasion of, host plants.

Keywords

plant parasitic nematodesMeloidogyne sppGlobodera rostochiensisplant cysteine proteinasesin vitronematicide
Type
Research Article
Information
Parasitology , Volume 134 , Issue 12 , November 2007 , pp. 1831 - 1838
Copyright
Copyright © Cambridge University Press 2007

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