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Morphological and molecular characterization of Trypanosoma copemani n. sp. (Trypanosomatidae) isolated from Gilbert's potoroo (Potorous gilbertii) and quokka (Setonix brachyurus)

Published online by Cambridge University Press:  06 May 2009

J. M. AUSTEN ,
R. JEFFERIES ,
J. A. FRIEND ,
U. RYAN ,
P. ADAMS  and
S. A. REID
J. M. AUSTEN
Affiliation:
School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, South Street, Murdoch, Western Australia, 6150
R. JEFFERIES
Affiliation:
School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
J. A. FRIEND
Affiliation:
Science Division, Department of Environment and Conservation, 120 Albany Highway, Albany, Western Australia, 6330
U. RYAN
Affiliation:
School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, South Street, Murdoch, Western Australia, 6150
P. ADAMS
Affiliation:
School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, South Street, Murdoch, Western Australia, 6150
S. A. REID*
Affiliation:
School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, South Street, Murdoch, Western Australia, 6150
*
*Corresponding author: School of Veterinary and Biomedical Sciences, Division of Health Sciences, Murdoch University, South Street, Murdoch, Western Australia, 6150. Tel: +61 8 9360 7423. Fax: +61 8 9310 4144. E-mail: [email protected]
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Summary

Little is known of the prevalence and life-cycle of trypanosomes in mammals native to Australia. Native Australian trypanosomes have previously been identified in marsupials in the eastern states of Australia, with one recent report in brush-tailed bettongs (Bettongia penicillata), or woylie in Western Australia in 2008. This study reports a novel Trypanosoma sp. identified in blood smears, from 7 critically endangered Gilbert's potoroos (Potorous gilbertii) and 3 quokkas (Setonix brachyurus) in Western Australia. Trypanosomes were successfully cultured in vitro and showed morphological characteristics similar to members of the subgenus Herpetosoma. Phylogenetic analysis of 18S rRNA gene sequences identified 2 different novel genotypes A and B that are closely related to trypanosomes previously isolated from a common wombat (Vombatus ursinus) in Victoria, Australia. The new species is proposed to be named Trypanosoma copemani n. sp.

Keywords

Trypanosoma copemanitrypanosomemarsupialhaemoparasitePotorous gilbertiiSetonix brachyurus
Type
Research Article
Information
Parasitology , Volume 136 , Issue 7 , June 2009 , pp. 783 - 792
Copyright
Copyright © Cambridge University Press 2009

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