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Monoamine oxidase in adult Hymenolepis diminuta (Cestoda)

Published online by Cambridge University Press:  06 April 2009

M. Sanchez Moreno  and
J. Barrett
M. Sanchez Moreno
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed, SY23 3DA
J. Barrett
Affiliation:
Department of Zoology, University College of Wales, Aberystwyth, Dyfed, SY23 3DA
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Summary

A membrane-bound monoamine oxidase (EC 1.4.3.4) was demonstrated in homogenates of Hymenolepis diminuta. The enzyme oxidized a variety of biologically active amines (in decreasing order: dopamine, adrenaline, noradrenaline, tryptamine, tyramine, octopamine), there was, however, no activity with 5-hydroxytryptamine or benzylamine. No diamine oxidase (EC 1.4.3.6) could be detected in H. diminuta (using histamine, cadaverine or putrescine as substrates). The monoamine oxidase from H. diminuta was not inhibited by azide, hydroxylamine or semicarbazide, but was inhibited by cupferron, α-α dipyridyl and iodoacetamide, and by the specific monoamine oxidase inhibitors pargyline, nialamide and iproniazid. Several anthelmintics were also found to be inhibitors of monoamine oxidase. The possible roles of monoamine oxidase in H. diminuta are discussed.

Type
Research Article
Information
Parasitology , Volume 78 , Issue 1 , February 1979 , pp. 1 - 5
Copyright
Copyright © Cambridge University Press 1979

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References

REFERENCES

Blashko, H. (1963). Amine oxidase. In The Enzymes, vol 8 (ed. Boyer, P. D., Lardy, H. and Myrbäck, K.), pp. 337–51. New York: Academic Press.Google Scholar
Chou, T. C. T., Bennett, J. & Bueding, E. (1972). Occurrence and concentrations of biogenic amines in trematodes. Journal of Parasitology 58, 1098–1102.CrossRefGoogle ScholarPubMed
Hariri, M. (1974). Occurrence and concentration of biogenic amines in Mesocestoides corti (Cestoda). Journal of Parasitology 60, 737–43.CrossRefGoogle ScholarPubMed
Layne, E. (1957). Spectrophotometric and turbidimetric methods for measuring proteins. In Methods in Enzymology, vol. 3 (ed. Colowick, S. P. and Kaplan, N. O.), pp. 447–54. New York: Academic Press.Google Scholar
Nimmo-Smith, R. H. & Raison, C. G. (1968). Monoamine oxidase activity of Schistosoma mansoni. Comparative Biochemistry and Physiology 24, 403–16.CrossRefGoogle ScholarPubMed
Shield, J. M. (1971). Histochemical localization of monoamines in the nervous system of Dipylidium caninum (Cestoda) by the formaldehyde fluorescence technique. International Journal for Parasitology 1, 135–8.CrossRefGoogle ScholarPubMed
Shih, J. H. C. & Eiduson, S. (1969). Multiple forms of monoamine oxidase in the developing brain. Nature, London 224, 1309–10.CrossRefGoogle ScholarPubMed
Smyth, J. D. (1969). The Physiology of Cestodes. Edinburgh: Oliver and Boyd.Google Scholar
Sweetman, A. J. & Weetman, D. F. (1972). The oxidation of biologically important monoamines by monoamine oxidase. In Experiments in Physiology and Biochemistry, vol. 5 (ed. Kerkut, G. A.), pp. 302–28. New York: Academic Press.Google Scholar
Tomosky-Sykes, T. K., Mueller, J. F. & Bueding, E. (1977). Effects of putative neurotransmitters on the motor activity of Spirometra mansonoides. Journal of Parasitology 63, 492–4.CrossRefGoogle ScholarPubMed
Yasunobu, K. T. & Gomes, B. (1971). Mitochondrial amine oxidase (monoamine oxidase) (beef liver). In Methods in Enzymology, vol. 17B (ed. Tabor, H. and Tabor, C. W.), pp. 709–22. New York: Academic Press.Google Scholar