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Molecular investigation of the Pfmdr1 gene of Plasmodium falciparum isolates in Henan Province imported from Africa

Published online by Cambridge University Press:  27 September 2018

Chengyun Yang Open the ORCID record for Chengyun Yang [Opens in a new window] ,
Ruimin Zhou ,
Ying Liu ,
Suhua Li ,
Dan Qian ,
Yuling Zhao ,
Hongwei Zhang  and
Bianli Xu
Chengyun Yang
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Ruimin Zhou
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Ying Liu
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Suhua Li
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Dan Qian
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Yuling Zhao
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Hongwei Zhang*
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Bianli Xu
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
*
Author for correspondence: Hongwei Zhang, E-mail: [email protected]
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Abstract

Efficacious antimalarial drugs are important for malaria control and elimination, and continuous monitoring of their efficacy is essential. The prevalence and distribution of Pfmdr1 were evaluated in African migrant workers in Henan Province. Among 632 isolates, 13 haplotypes were identified, NYSND (39.87%, 252/632), YYSND (2.85%, 18/632), NFSND (31.01%, 196/632), NYSNY (0.47%, 3/632), YFSND (13.77%, 87/632), NFSNY (0.32%, 2/632), YYSNY (2.06%, 13/632), YFSNY (0.16%, 1/632), N/Y YSND (1.90%, 12/632), N Y/F SND (6.17%, 39/632), N/Y Y/F SND (0.47%, 3/632), YYSN D/Y (0.16%, 1/632) and N/Y FSND (0.79%, 5/632). The highest frequency of NYSND was observed in individuals from North Africa (63.64%, 7/11), followed by South Africa (61.33%, 111/181), Central Africa (33.33%, 56/168), West Africa (28.94%, 68/235) and East Africa (27.03%, 10/37) (χ2 = 54.605, P < 0.05). The highest frequency of NFSND was observed in East Africa (48.65%, 18/37), followed by West Africa (39.14%, 92/235), Central Africa (26.79%, 45/168), South Africa (22.65%, 41/181) and North Africa (9.09%, 1/11) (χ2 = 22.368 P < 0.05). The mutant prevalence of codons 86 and 184 decreased. These data may provide complementary information on antimalarial resistance that may be utilized in the development of a treatment regimen for Henan Province.

Keywords

Africaantimalarial drugsHenan ProvincemalariaPfmdr1Plasmodium falciparum
Type
Research Article
Information
Parasitology , Volume 146 , Issue 3 , March 2019 , pp. 372 - 379
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Copyright
Copyright © Cambridge University Press 2018 

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References

Conrad, MD, LeClair, N, Arinaitwe, E, Wanzira, H, Kakuru, A, Bigira, V, Muhindo, M, Kamya, MR, Tappero, JW and Greenhouse, B (2014) Comparative impacts over 5 years of artemisinin-based combination therapies on Plasmodium falciparum polymorphisms that modulate drug sensitivity in Ugandan children. Journal of Infectious Diseases 210, 344–353.Google Scholar
Danquah, I, Coulibaly, B, Meissner, P, Petruschke, I, Muller, O and Mocken-haupt, FP (2010) Selection of pfmdr1 and pfcrt alleles in amodiaquine treatment failure in in north-western Burkina Faso. Acta Tropica 114, 63–66.Google Scholar
Dokomajilar, C, Lankoande, ZM, Dorsey, G, Zongo, I, Ouedraogo, JB and Rosenthal, PJ (2006) Roles of specific Plasmodium falciparum mutations in resistance to amodiaquine and sulfadoxine-pyrimethamine in Burkina Faso. American Journal of Tropical Medicine and Hygiene 75, 162–165.Google Scholar
Feng, J, Yan, H, Feng, XY, Zhang, L, Li, M, Xia, ZG and Xiao, N (2014) Imported malaria in China, 2012. Emerging Infectious Diseases 20, 1778–1780.Google Scholar
Feng, J, Li, J, Yan, H, Feng, MY and Xia, ZG (2015) Evaluation of antimalarial resistance marker polymorphism in returned migrant workers in China. Antimicrobial Agents and Chemotherapy 59, 326–330.Google Scholar
Froberg, G, Jornhagen, L, Morris, U, Shakely, D, Msellem, MI, Gil, JP, Bjorkman, A and Martensson, A (2012) Decreased prevalence of Plasmodium falciparum resistance markers to amodiaquine despite its wide scale use as ACT partner drug in Zanzibar. Malaria Journal 11, 321.Google Scholar
Gama, BE, Pereira-Carvalho, GA, Lutucuta Kosi, FJ, Almeida de Oliveira, NK, Fortes, F, Rosenthal, PJ, Daniel-Ribeiro, CT and Ferreira-da-Cruz, MF (2010) Plasmodium falciparum isolates from Angola show the StctVMNT haplotype in the pfcrt gene. Malaria Journal 9, 174.Google Scholar
Guan, YY, Tang, LH, Hu, L, Feng, XP and Liu, DQ (2005) The point mutations in Pfcrt and Pfmdr1 genes in Plasmodium falciparum isolated from Hainan Province (in Chinese). Chinese Journal of Parasitology and Parasitic Diseases 23, 135–139.Google Scholar
Henriques, G, Hallett, RL, Beshir, KB, Gadalla, NB, Johnson, RE, Burrow, R, Schalkwyk, DA, Sawa, P, Omar, SA, Clark, TG, Bousema, T and Sutherland, CJ (2014) Directional selection at the pfmdr1, pfcrt, pfubp1, and pfap2mu loci of Plasmodium falciparum in Kenyan children treated with ACT. Journal of Infectious Diseases 210, 2001–2008.Google Scholar
Huang, F, Tang, L, Yang, H, Zhou, S, Liu, H, Li, J and Guo, S (2012) Molecular epidemiology of drug resistance markers of Plasmodium falciparum in Yunnan Province, China. Malaria Journal 11, 243.Google Scholar
Lin, JT, Juliano, JJ and Wongsrichanalai, C (2010) Drug-resistant malaria: the era of ACT. Current Infectious Disease Reports 12, 165–173.Google Scholar
Liu, Y, Zhang, HW, Zhou, RM, Yang, CY, Qian, D, Zhao, YL and Xu, BL (2014 a) First imported relapse case of Plasmodium vivax malaria and analysis of its origin CSP sequencing in Henan Province, China. Malaria Journal 13, 448.Google Scholar
Liu, YB, Hsiang, MS, Zhou, HY, Wang, WM, Cao, YY, Gosling, RD, Cao, J and Gao, Q (2014 b) Malaria in overseas labourers returning to China: an analysis of imported malaria in Jiangsu Province, 2001–2011. Malaria Journal 13, 29.Google Scholar
Liu, Y, Zhou, RM, Qian, D, Yang, CY and Zhang, HW (2014 c) Analysis of malaria epidemiological characteristics in Henan Province from 2005 to 2013(In Chinese). Chinese Journal of Parasitology Parasitic Diseases 32, 419–422.Google Scholar
Lucchi, NW, Komino, F, Okoth, SA, Goldman, I, Onyona, P, Wiegand, PE, Juma, E, Shi, YP, Barnwell, JW, Udhayakumar, V and Kariuki, S (2015) In vitro and molecular surveillance for antimalarial drug resistance in Plasmodium falciparum parasites in Western Kenya reveals sustained artemisinin sensitivity and increased chloroquine sensitivity. Antimicrobial Agents and Chemotherapy 59, 7540–7547.Google Scholar
Maberti, S (1960) Development of resistance to pyrimethamine, presentation of 15 cases studied in Trujillo, Venezuela. Archivos Venezolanos De Medicina Tropical Y Parasitología Médica 3, 239–259.Google Scholar
Mehlotra, RK, Fujioka, H and Roepe, PD (2001) Evolution of a unique Plasmodium falciparum chloroquine-resistance phenotype in association with pfcrt polymorphism in Papua New Guinea and South America. Proceedings of the National Academy of Sciences of the USA 98, 12689–12694.Google Scholar
Mok, S, Imwong, M, Mackinnon, MJ, Sim, J, Ramadoss, R, Yi, P, Mayxay, M, Chotivanich, K, Liong, KY, Russell, B, Socheat, D, Newton, PN, Day, NPJ, White, NJ, Preiser, PR, Nosten, F, Dondorp, AM and Bozdech, Z (2011) Artemisinin resistance in Plasmodium falciparum is associated with an altered temporal pattern of transcription. BMC Genomics 12, 391.Google Scholar
Moore, DV and Lanier, JE (1961) Observations on the two Plasmodium falciparum infections with an abnormal response to chloroquine. American Journal of Tropical Medicine and Hygiene 10, 5–9.Google Scholar
Muller, O, Sie, A, Meissner, P, Schirmer, RH and Kouyate, B (2009) Artemisinin resistance on the Thai-Cambodian border. The Lancet 374, 1419.Google Scholar
Mvumbi, DM, Kayembe, J, Situakibanza, H, Bobanga, TL, Nsibu, CN, Mvumbi, GL, Melin, P, Mol, PD and Hayette, M (2015) Falciparum malaria molecular drug resistance in the Democratic Republic of Congo: a systematic review. Malaria Journal 14, 354.Google Scholar
Mwai, L, Kiara, SM, Abdirahman, A, Pole, L, Rippert, A, Diriye, A, Bull, P, Marsh, K, Borrmann, S and Nzila, A (2009) In vitro activities of piperaquine, lumefantrine, and dihydroartemisinin in Kenyan Plasmodium falciparum isolates and polymorphisms in pfcrt and pfmdr1. Antimicrobial Agents and Chemotherapy 53, 5069–5073.Google Scholar
Nsobya, SL, Dokomajilar, C, Joloba, M, Dorsey, G and Rosenthal, PJ (2007) Resistance-mediating Plasmodium falciparum pfcrt and pfmdr1 alleles after treatment with artesunate-amodiaquine in Uganda. Antimicrobial Agents and Chemotherapy 51, 3023–3025.Google Scholar
Ojurongbe, O, Ogungbamigbe, TO, Fagbenro-Beyioku, AF, Fendel, R, Kremsner, PG and Kun, JFJ (2007) Rapid detection of Pfcrt and Pfmdr1 mutations in Plasmodium falciparum isolates by FRET and in vivo response to chloroquine among children from Osogbo, Nigeria. Malaria Journal 6, 41.Google Scholar
Peters, W (1987) Chemotherapy and Drug Resistance in Malaria, 2nd Edn. London: Academic Press, p. 542.Google Scholar
Plucinski, MM, Talundzic, E, Morton, L, Dimbu, PR, Macaia, AP, Fortes, F, Goldman, I, Lucchi, N, Stennies, G, MacArthur, JR and Udhayakumar, V (2015) Efficacy of artemether-lumefantrine and dihydroartemisinin-piperaquine for treatment of uncomplicated malaria in children in Zaire and Uige Provinces, Angola. Antimicrobial Agents and Chemotherapy 59, 437–443.Google Scholar
Reyes, S (1981) Malarial infections with Plasmodium falciparum resistant to chloroquine treatment. The situation in Braxil (1960–1981). Revista brasileira de malariologia e doenças tropicais. Publicações Avulsas 33, 109–130.Google Scholar
Ridley, RG (2002) Medical need, scientific opportunity and the drive for anti-malarial drugs. Nature 415, 686–693.Google Scholar
Roper, C, Pearce, R, Bredenkamp, B, Gumede, J, Drakeley, C, Mosha, F, Chandramohan, D and Sharp, B (2003) Antifolate antimalarial resistance in southeast Africa: a population-based analysis. The Lancet 361, 1174–1181.Google Scholar
Sisowath, C, Ferreira, PE, Bustamante, LY, Dahlstrom, S, Martensson, A, Bjorkman, A, Krishna, S and Gil, JP (2007) The role of pfmdr1 in Plasmodium falciparum tolerance to artemether-lumefantrine in Africa. Tropical Medicine & International Health 12, 736–742.Google Scholar
Thomas, SM, Ndir, O, Dieng, T, Mboup, S, Wypij, D, Maguire, JH and Wirth, DF (2002) In vitro chloroquine susceptibility and PCR analysis of Pfcrt and Pfmdr1 polymorphisms in Plasmodium falciparum isolates from Senegal. American Journal of Tropical Medicine and Hygiene 66, 474–480.Google Scholar
Trape, JF, Pison, G, Spiegel, A, Enel, C and Rogier, C (2002) Combating malaria in Africa. Trends in Parasitology 18, 224–230.Google Scholar
Van Tyne, D, Dieye, B, Valim, C, Daniels, RF, Sene, PD, Lukens, AK, Ndiaye, M, Bei, AK, Ndiaye, YD, Hamilton, EJ, Ndir, O, Mboup, S, Volkman, SK, Wirth, DF and Ndiaye, D (2013) Changes in drug sensitivity and anti-malarial drug resistance mutations over time among Plasmodium falciparum parasites in Senegal. Malaria Journal 12, 441.Google Scholar
Vitoria, M, Granich, R, Gilks, CF, Gunneberq, C, Hosseini, M, Were, W, Raviqlione, M and De Cock, KM (2009) The global fight against HIV/AIDS, tuberculosis, and malaria: current status and future perspectives. American Journal of Clinical Pathology 131, 844–848.Google Scholar
Wang, ZL, Shrestha, S, Li, XL, Miao, J, Yuan, LL, Cabrera, M, Grube, C, Yang, ZQ and Cui, LW (2015) Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007–2012. Malaria Journal 14, 168.Google Scholar
Wellems, TE and Plowe, CV (2001) Chloroquine resistant malaria. Journal of Infectious Diseases 184, 770–776.Google Scholar
Wernsdorfer, WH and Payne, D (1991) The dynamics of drug resistance in Plasmodium falciparum. Pharmacology and Therapeutics 50, 95–121.Google Scholar
WHO (2006) Guidelines for the Treatment of Malaria. Geneva: World Health OrganizationGoogle Scholar
WHO (2016) World malaria report 2016. Geneva: World Health Organization.Google Scholar
Xu, C, Wei, QK, Li, J, Xiao, T, Yin, K, Kong, XL, Wang, YB, Cui, Y, Sun, H, Zhao, GH, Zhu, X, Yan, C and Huang, BC (2016) Haplotype and mutation analysis of drug resistance Pfcrt and Pfmdr1 gene of imported Plasmodium falciparum (In Chinese). Chinese Journal of Zoonoses 32, 1051–1057.Google Scholar
Yang, CY, Qian, D, Chen, WQ, Liu, Y, Zhou, RM and Zhang, HW (2016) Investigation and analysis of overseas imported malaria prevalence in Henan Province from 2012 to 2014(In Chinese). Chinese Journal of Schistosomiasis Control 28, 444–446.Google Scholar
Zhang, L, Zhou, SS, Feng, J, Fang, W and Xia, ZG (2015) Malaria situation in the People's Republic of China in 2014 (In Chinese). Chinese Journal of Parasitology and Parasitic Diseases 33, 321–326.Google Scholar
Zhou, RM, Zhang, HW, Yang, CY, Liu, Y, Zhao, YL, Li, SH, Qian, D and Xu, BL (2016) Molecular mutation profile of pfcrt in plasmodium falciparum isolates imported from Africa in Henan Province. Malaria Journal 15, 265.Google Scholar