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Molecular detection of Babesia spp. and other haemoparasitic infections of cattle in Maputo Province, Mozambique

Published online by Cambridge University Press:  04 February 2010

TIAGO M. MARTINS ,
LUÍS NEVES ,
OLÍVIA C. PEDRO ,
JOSÉ M. FAFETINE ,
VIRGÍLIO E. DO ROSÁRIO  and
ANA DOMINGOS
TIAGO M. MARTINS*
Affiliation:
Unidade de Tecnologias de Proteínas e Anticorpos Monoclonais, Instituto de Higiene e Medicina Tropical, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Rua da Junqueira 96, 1349-008 Lisboa, Portugal
LUÍS NEVES
Affiliation:
Centro de Biotecnologia, Universidade Eduardo Mondlane, Av. de Moçambique km 1.5, Maputo, Mozambique
OLÍVIA C. PEDRO
Affiliation:
Centro de Biotecnologia, Universidade Eduardo Mondlane, Av. de Moçambique km 1.5, Maputo, Mozambique Faculdade de Veterinária, Universidade Eduardo Mondlane, Av. de Moçambique km 1.5, Maputo, Mozambique
JOSÉ M. FAFETINE
Affiliation:
Centro de Biotecnologia, Universidade Eduardo Mondlane, Av. de Moçambique km 1.5, Maputo, Mozambique Faculdade de Veterinária, Universidade Eduardo Mondlane, Av. de Moçambique km 1.5, Maputo, Mozambique
VIRGÍLIO E. DO ROSÁRIO
Affiliation:
Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Rua da Junqueira 96, 1349-008 Lisboa, Portugal
ANA DOMINGOS
Affiliation:
Unidade de Tecnologias de Proteínas e Anticorpos Monoclonais, Instituto de Higiene e Medicina Tropical, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Rua da Junqueira 96, 1349-008 Lisboa, Portugal
*
*Corresponding author: Unidade de Tecnologias de Proteínas e Anticorpos Monoclonais, Instituto de Higiene e Medicina Tropical, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal. Tel: +351 210924712. Fax: +351 217163636. E-mail: [email protected]
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Summary

Molecular detection of Babesia species in apparently healthy cattle within an endemic region was carried out in order to determine the prevalence of carriers and the geographical distribution of Babesia bigemina and Babesia bovis in Maputo Province, Mozambique. Samples from 477 animals at 5 localities were analysed using 2 techniques, the semi-nested hot-start PCR and the reverse line blot (RLB) assay. With the semi-nested hot-start PCR, detection of B. bigemina ranged between 30% and 89%, and of B. bovis between 27% and 83%. The RLB assay was comparatively less sensitive in this study and detection of B. bovis ranged from 0% to 17%, and B. bigemina was not detected at all by this technique. Analysis of new sequences of the 18S rRNA gene revealed that the current B. bigemina RLB probe is not specific for the identification of isolates in Mozambique. The RLB assay, however, resulted in the detection of 8 other haemoparasite species belonging to the genera Babesia, Theileria, Anaplasma and Ehrlichia. 18S rRNA gene sequences from the Theileria spp. were identified, and a phylogenic tree constructed with these sequences yielded a heterogeneous T. mutans-like group. In conclusion, infection with B. bigemina and B. bovis is endemic in Maputo Province, but rates of transmission vary. Furthermore, mixed infections with the haemoparasites responsible for several tick-borne diseases in cattle are common in Mozambique.

Keywords

tick-borne diseasescattleBabesia bigeminaBabesia bovishot-start PCRRLB
Type
Research Article
Information
Parasitology , Volume 137 , Issue 6 , May 2010 , pp. 939 - 946
Copyright
Copyright © Cambridge University Press 2010

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