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Molecular characterization of Thelastomatoidea (Nematoda: Oxyurida) from cockroaches in Australia

Published online by Cambridge University Press:  27 March 2006

A. R. JEX ,
M. HU ,
H. A. ROSE ,
M. SCHNEIDER ,
T. H. CRIBB  and
R. B. GASSER
A. R. JEX
Affiliation:
School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Queensland, Australia
M. HU
Affiliation:
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
H. A. ROSE
Affiliation:
School of Land, Water and Crop Sciences, The University of Sydney, Camperdown, New South Wales, Australia
M. SCHNEIDER
Affiliation:
Department of Zoology and Entomology, The University of Queensland, St Lucia, Queensland, Australia
T. H. CRIBB
Affiliation:
School of Molecular and Microbial Sciences, The University of Queensland, St Lucia, Queensland, Australia
R. B. GASSER
Affiliation:
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
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Abstract

A molecular approach was used to genetically characterize 5 species (Aoruroides queenslandensis, Blattophila sphaerolaima, Cordonicola gibsoni, Desmicola ornata and Leidynemella fusiformis) belonging to the superfamily Thelastomatoidea (Nematoda: Oxyurida), a group of pinworms that parasitizes terrestrial arthropods. The D3 domain of the large subunit of nuclear ribosomal RNA (LSU) was sequenced for individual specimens, and the analysis of the sequence data allowed the genetic relationships of the 5 species to be studiedNucleotide sequence data reported in this paper are available in the GenBank®, EMBL and DDBJ databases under the Accession numbers: AM232755–AM232763.. The sequence variation in the D3 domain within individual species (0–1·8%) was significantly less than the differences among species (4·3–12·4%). Phylogenetic analyses, using maximum parsimony, maximum likelihood, and neighbour-joining, tree-building methods, established relationships among the 5 species of Thelastomatoidea and Oxyuris equi (a species of the order Oxyurida). The molecular approach employed provides the prospect for developing DNA tools for the specific identification of the Thelastomatoidea, irrespective of developmental stage and sex, as a basis for systematic, ecological and/or population genetic investigations of members within this superfamily.

Keywords

genetic variationThelastomatoideaOxyuridaNematodaD3 domainlarge subunit of nuclear ribosomal RNA
Type
Research Article
Information
Parasitology , Volume 133 , Issue 1 , July 2006 , pp. 123 - 129
Copyright
2006 Cambridge University Press

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