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Molecular characterization of Giardia duodenalis isolated from Semai Pahang Orang Asli (Peninsular Malaysia aborigines)

Published online by Cambridge University Press:  07 August 2009

A. K. MOHAMMED MAHDY ,
JOHARI SURIN ,
A. MOHD-ADNAN ,
K.-L. WAN  and
Y. A. L. LIM
A. K. MOHAMMED MAHDY*
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Department of Parasitology, Faculty of Medicine, Sana'a University, Sana'a, Yemen
JOHARI SURIN
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
A. MOHD-ADNAN
Affiliation:
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia
K.-L. WAN
Affiliation:
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia
Y. A. L. LIM
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
*
*Corresponding author: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Tel: +603 7967 4746. Fax: +603 7967 4754. E-mail: [email protected]
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Summary

This study was conducted to determine the genotypes of Giardia duodenalis isolated from human faecal samples at Pos Betau, Pahang, Malaysia. Faecal specimens were collected and examined for G. duodenalis cysts using Trichrome staining techniques. Molecular identification was carried out by the amplification of a region of the small subunit of the nuclear ribosomal RNA (SSU rRNA) gene using nested PCR and subsequent sequencing. The sequences from 15 isolates from G. duodenalis were subjected to phylogenetic analysis (including appropriate outgroups) using the neighbor-joining and maximum parsimony methods. The trees identified G. duodenalis assemblages A and B, with a predominance of assemblage B. The predominance of anthroponotic genotypes indicates the possibility of anthroponotic transmission of these protozoa in this Semai Pahang Orang Asli community.

Keywords

Giardia duodenalisassemblage BgenotypesSemai Pahang Orang Asli
Type
Research Article
Information
Parasitology , Volume 136 , Issue 11 , September 2009 , pp. 1237 - 1241
Copyright
Copyright © Cambridge University Press 2009

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