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Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum

Published online by Cambridge University Press:  01 December 1997

B. COUVREUR ,
D. JACQUET ,
A. BOLLEN  and
D. LE RAY
B. COUVREUR
Affiliation:
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium Service de Génétique Appliquée, Université Libre de Bruxelles, rue de l'Industrie 24, B-1400 Nivelles, Belgium
D. JACQUET
Affiliation:
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium
A. BOLLEN
Affiliation:
Service de Génétique Appliquée, Université Libre de Bruxelles, rue de l'Industrie 24, B-1400 Nivelles, Belgium
D. LE RAY
Affiliation:
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium
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Abstract

Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS–PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6–9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.

Keywords

Leishmania infantumserodiagnosisimmunoelectrophoresisimmunodominant antigen
Type
Research Article
Information
Parasitology , Volume 115 , Issue 6 , December 1997 , pp. 611 - 619
Copyright
1997 Cambridge University Press

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