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Molecular and morphological variation of Paragonimus westermani in Vietnam with records of new second intermediate crab hosts and a new locality in a northern province

Published online by Cambridge University Press:  21 July 2016

N. PHAM DOANH ,
A. LUU TU ,
T. DUNG BUI ,
T. HO LOAN ,
NARIAKI NONAKA ,
YOICHIRO HORII ,
DAVID BLAIR  and
YUKIFUMI NAWA
N. PHAM DOANH*
Affiliation:
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
A. LUU TU
Affiliation:
Vietnam National Lung Hospital, Hanoi, Vietnam
T. DUNG BUI
Affiliation:
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
T. HO LOAN
Affiliation:
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
NARIAKI NONAKA
Affiliation:
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan
YOICHIRO HORII
Affiliation:
Laboratory of Veterinary Parasitic Diseases, Faculty of Agriculture, University of Miyazaki, Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan
DAVID BLAIR
Affiliation:
College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia
YUKIFUMI NAWA
Affiliation:
Research Affairs Office, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
*
*Corresponding author: Department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam. E-mail: [email protected]
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Summary

Paragonimus westermani is one of the most medically important lung flukes and is widely distributed in Asia. It exhibits considerable variation in morphological, genetic and biological features. In central provinces of Vietnam, a high prevalence of metacercariae of this species has been reported from the crab intermediate host, Vietopotamon aluoiense. In this study, we detected P. westermani metacercariae in two additional crab hosts, Donopotamon haii in Quang Tri Province, central Vietnam and Indochinamon tannanti in Yen Bai Province in the north. The latter is a new locality for P. westermani in a northern region of Vietnam where P. heterotremus is the only species currently known to cause human paragonimiasis. Paragonimus westermani metacercariae found in Vietnam showed considerable morphological variation but slight genetic variation based on DNA sequences from the nuclear ribosomal ITS2 region and the mitochondrial 16S gene. Co-infection of the same individual crabs with P. westermani and P. heterotremus and/or some other Paragonimus species was found frequently, suggesting potential for co-infection in humans. The findings of the present study emphasize the need for highly specific molecular and immunodiagnostic methods to differentially diagnose between P. westermani and P. heterotremus infections.

Keywords

Paragonimus westermanimorphological and molecular variationnew localitynew crab intermediate hosts
Type
Research Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1639 - 1646
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Copyright
Copyright © Cambridge University Press 2016 

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References

REFERENCES

Agatsuma, T., Iwagami, M., Sato, Y., Iwashita, J., Hong, S. J., Kang, S. Y., Ho, L. Y., Su, K. S., Kawashima, K. and Abe, T. (2003). The origin of the triploid in Paragonimus westermani on the basis of variable regions in the mitochondrial DNA. Journal of Helminthology 77, 279–285.CrossRefGoogle ScholarPubMed
Binchai, S., Rangsiruji, A., Ketudat, P., Morishima, Y. and Sugiyama, H. (2007). Molecular systematic of a new form of Paragonimus westermani discovered in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 38 (Suppl. 1), 92–96.Google Scholar
Blair, D., Agatsuma, T., Watanobe, T., Okamoto, M. and Ito, A. (1997). Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences. Parasitology 115, 411–417.Google Scholar
Blair, D., Xu, Z. B. and Agatsuma, T. (1999). Paragonimiasis and the genus Paragonimus . Advance in Parasitology 42, 113–222.Google Scholar
Blair, D., Nawa, Y., Mitreva, M. and Doanh, P. N. (2016). Gene diversity and genetic variation in lung flukes (genus Paragonimus). Transactions of the Royal Society of Tropical Medicine and Hygiene 110, 6–12.Google Scholar
Bowles, J., Blair, D. and McManus, D. P. (1995). A molecular phylogeny of the human schistosomes. Molecular Phylogenetics and Evolution 4, 103–109.CrossRefGoogle ScholarPubMed
Dang, N. T. and Ho, T. H. (2012). Freshwater Shrimps and Crabs in Vietnam (Palaemonidae, Atyidae, Parathelphusidae, Potamidae). Publishing House for Science and Technology (in Vietnamese), Hanoi.Google Scholar
Dang, N. T., Thai, T. B. and Pham, V. M. (1980). Identification of Invertebrates in Northern Vietnam. Publishing house for Science and technology, Hanoi (in Vietnamese).Google Scholar
De, N. V., Murrell, K. D., Cong, L. D., Cam, P. D., Chau, L. V., Toan, N. D. and Dalsgaard, A. (2003). The food-borne trematodes zoonoses of Vietnam. Southeast Asian Journal of Tropical Medicine and Public Health 34, 12–34.Google ScholarPubMed
Devi, K. R., Narain, K., Agatsuma, T., Blair, D., Nagataki, M., Wickramasinghe, S., Yatawara, L. and Mahanta, J. (2010). Morphological and molecular characterization of Paragonimus westermani in northeastern India. Acta Tropica 116, 31–38.Google Scholar
Devi, K. R., Narain, K., Mahanta, J., Nirmolia, T., Blair, D., Saikia, S. P. and Agatsuma, T. (2013). Presence of three distinct genotypes within the Paragonimus westermani complex in northeastern India. Parasitology 140, 76–86.Google Scholar
Doanh, P. N., Shinohara, A., Horii, Y., Habe, S., Nawa, Y., The, D. T. and Le, N. T. (2007). Morphological and molecular identification of two Paragonimus spp., of which metacercariae concurrently found in a land crab, Potamiscus tannanti, collected in Yenbai Province, Vietnam. Parasitology Research 100, 1075–1082.CrossRefGoogle Scholar
Doanh, P. N., Shinohara, A., Horii, Y., Habe, S. and Nawa, Y. (2009). Discovery of Paragonimus westermani in Viet Nam and its molecular phylogenetic status in P. westermani complex. Parasitology Research 104, 1149–1155.Google Scholar
Doanh, P. N., Dung, D. T., Thach, D. T., Horii, Y., Shinohara, A. and Nawa, Y. (2011). Human paragonimiasis in Viet Nam: epidemiological survey and identification of the responsible species by DNA sequencing of eggs in patients’ sputum. Parasitology International 60, 534–537.Google Scholar
Doanh, P. N., Horii, Y. and Nawa, Y. (2013). Paragonimus and paragonimiasis in Vietnam: an update. Korean Journal of Parasitology 51, 621–627.CrossRefGoogle ScholarPubMed
Iwagami, M., Ho, L. Y., Su, K., Lai, P. F., Fukushima, M., Nakano, M., Blair, D., Kawashima, K. and Agatsuma, T. (2000). Molecular phylogeographic studies on Paragonimus westermani in Asia. Journal of Helminthology 74, 315–322.Google Scholar
Iwagami, M., Rajapakse, R. P. V. J., Paranagama, W., Okada, T., Kano, S. and Agatsuma, T. (2008). Ancient divergence of Paragonimus westermani in Sri Lanka. Parasitology Research 102, 845–852.Google Scholar
Kawashima, K., Hua, J. and Ho, L. Y. (1989). Paragonimus in China. In Paragonimus in Asia: Biology, Genetic Variation and Speciation (II) (ed. Kawashima, K.), pp. 2–9. Shunpposha Photographic Printing Co. Ltd, Fukuoka.Google Scholar
Landmann, H., Ngu, D. V. and Thai, D. D. (1961). Paragonimiasis in Vietnam. Wochenschrqt fÜr die Gesumre Medizin 16, 1355–1364 (in German).Google Scholar
Le, T. H., De, N. V., Blair, D., McManus, D. P., Kino, H. and Agatsuma, T. (2006). Paragonimus heterotremus Chen et Hsia, 1964 in Vietnam: a molecular identification and relationships of isolates from different hosts and geographical origins. Acta Tropica 98, 25–33.Google Scholar
Miyazaki, I. (1978). Two types of the lung fluke which has been called Paragonimus westermani (Kerbert, 1878). Medical Bulletin of Fukuoka University 5, 251–263.Google Scholar
Miyazaki, I. (1991). An Illustrated Book of Helminthic Zoonoses. International Medical Foundation of Japan, Tokyo.Google Scholar
Nawa, Y., Thaenkham, U., Doanh, P. N. and Blair, D. (2014). Paragonimus westermani and Paragonimus species. In Encyclopedia of Food Safety, vol. 2 (ed. Motarjemi, Y.), pp. 179–187. Elsevier, Amsterdam, Netherlands.Google Scholar
Park, G. M., Lee, K. J., Im, K. I., Park, H. and Yong, T. S. (2001). Occurrence of a diploid type and a new first intermediate host of a human lung fluke, Paragonimus westermani, in Korea. Experimental Parasitology 99, 206–212.Google Scholar
Singh, T. S., Hiromu, S., Devi, K. R. and Singh, W. A. (2015). First case of Paragonimus westermani infection in a female patient in India. Indian Journal of Medical Microbiology 33 (Suppl. 1), 156–159.Google Scholar
Sugiyama, H., Morishima, Y., Binchai, S., Rangsiruji, A. and Ketudat, P. (2007). A new form of Paragonimus westermani discovered in Thailand: morphological characteristics and host susceptibility. Southeast Asian Journal of Tropical Medicine and Public Health 38 (Suppl. 1), 87–91.Google Scholar
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.Google Scholar