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Mode of nutrition of the larvae of the filarial nematode Brugia pahangi

Published online by Cambridge University Press:  06 April 2009

Evelyn B. Beckett  and
B. Boothroyd
Evelyn B. Beckett
Affiliation:
Department of Histology, University of Liverpool
B. Boothroyd
Affiliation:
Department of Histology, University of Liverpool
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Summary

(1) Light-microscopic observations were made on B. pahangi larvae at various stages of development in stained serial sections of A. aegypti thoraces, and on 8-to 9-day 2nd-stage live, unstained larvae.

(2) Six-day larvae situated in A. aegypti flight muscle, and isolated 8- to 9-day 2nd-stage larvae, were examined with the electron microscope.

(3) Results showed that host-muscle mitochondria which appeared to be morphologically intact aggregated outside the larvae at the head and tail ends and were present from 6 days onwards in the oesophagus and in the midgut. Those in the oesophagus were unchanged; some of those in the midgut were intact, others showed varying degrees of disintegration suggestive of digestion. Host-muscle mitochondria are therefore likely to be a source of nutrients for B. pahangi larvae during the last third of their intramuscular developmental phase.

Dr W. W. Macdonald, of the Liverpool School of Tropical Medicine, kindly supplied the infected mosquitoes, and offered valuable advice in the course of this work. Miss M. A. Johnson assisted with the care of the mosquitoes.

We are particularly indebted to Mr David John for his patient work in preparing the filariae for electron-microscopic observation. Mrs J. Oliver assisted with some of the histological preparations.

We are pleased to acknowledge the financial assistance of the Royal Society towards the purchase of the ultramicrotomes used in the course of this work.

Type
Research Article
Information
Parasitology , Volume 60 , Issue 1 , February 1970 , pp. 21 - 26
Copyright
Copyright © Cambridge University Press 1970

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References

Beckett, E. B. (1965). Histochemical observations on Aedes aegypti infected with larvae of Brugia malayi. Transactions of the Royal Society of Tropical Medicine and Hygiene 59, 461–71.Google Scholar
Glauert, A. M. & Glauert, R. M. (1958). Araldite as an embedding medium for electron microscopy. Journal of Biophysical and Biochemical Cytology 4, 191–4.CrossRefGoogle ScholarPubMed
Iyengar, R. (1956 a). Développement de Wuchereria bancrofti (Cobbold) et de Wuchereria malayi (Brug) Ière partie. Annales de parasitologie humaine et comparée 31, 99–138.CrossRefGoogle Scholar
Iyengar, R. (1956 b). Développment de Wuchereria bancrofti (Cobbold) et de Wuchereria malayi (Brug) IIe partie. Annales de parasitologie humaine et comparee 31, 266–87.Google Scholar
Laurence, B. R. & Pester, F. R. N. (1961). The ability of Anopheles gambiae Giles to transmit Brugia patei (Buckley, Nelson & Heisch). Journal of Tropical Medicine and Hygiene 64, 169–71.Google Scholar
Macdonald, W. W. & Ramachandran, C. P. (1965). The influence of the gene fm (filarial susceptibility, Brugia malayi) on the susceptibility of Aedes aegypti to seven strains of Brugia, Wuchereria and Dirofilaria. Annals of Tropical Medicine and Parasitology 59, 64–73.CrossRefGoogle ScholarPubMed
Nelson, G. S. (1964). Factors influencing the development and behaviour of filarial nema-todes in their arthropodan hosts. In Host-parasite Relationships in Invertebrate Hosts: 2nd Symposium of the British Society of Parasitology, ed. Taylor, A. E. R.. Oxford: Blackwell.Google Scholar
Schacher, J. F. (1962). Morphology of the microfilariae of Brugia pahangi and of the larval stages in the mosquito. Journal of Parasitology 48, 679–92.Google Scholar
Webber, W. A. F. (1955). Dirofilaria aethiops Webber 1955, a filarial parasite of monkeys. III. The larval development in mosquitoes; Parasitology 45, 388–399.Google Scholar
Williams, P. (1960). Studies on the Ethiopian Chrysops as possible vectors of loiasis. II. Chrysops silacea Austen and human loiasis. (Part I.) Annals of Tropical Medicine and Parasitology 54, 439–59.Google Scholar