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Migration of the schistosomula of Schistosoma mansoni from the lungs to the hepatic portal system

Published online by Cambridge University Press:  06 April 2009

Patricia Miller  and
R. A. Wilson
Patricia Miller
Affiliation:
Department of Biology, University of York, Heslington, York
R. A. Wilson
Affiliation:
Department of Biology, University of York, Heslington, York
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Summary

The pattern of recovery of schistosomula from the lungs of mice, hamsters and rats is described following normal percutaneous infection via the abdominal skin. Peak numbers were found in hamsters and rats on day 5 and in mice on day 6 post-exposure. Schistosomula were recovered from the lungs up to approximately day 20 post-exposure in all 3 species. None were found in pleural washings and only 2 were recovered following mincing and incubation of host diaphragms. The pattern of recovery of schistosomula from the lungs of mice was also described following the injection of a pulse of parasites into the tail vein. Approximately 54% of these injected schistosomula eventually reached maturity in the hepatic portal system. The ability of schistosomula to migrate and mature following their injection into unusual locations was tested. Small numbers reached maturity in the hepatic portal system following injection into the pleural cavity or subcutaneously, more when injected intra-peritoneally and largest numbers following intravenous injection. Small numbers of schistosomula were recovered by mincing and incubation of systemic organs such as the kidney and spleen. Schistosomula injected into the left ventricle of hamsters were able to migrate via the systemic organs to the lungs and 47% eventually matured in the hepatic portal system. The pattern of schistosomulum accumulation in the hepatic portal system of mice, hamsters and rats is described. Following normal percutaneous infection, schistosomula were first detected in this site from day 6 to day 9 depending on the host species. In mice, migration was complete around day 20 but continued in hamsters up to day 40 post-exposure. Following injection of schistosomula into the tail veins of mice, the first arrivals in the hepatic portal system were detected 12 h later and were found to lose their ability to migrate shortly after arrival. However, a proportion of lung schistosomula injected directly into the hepatic portal system were able to traverse hepatic sinusoids and reach the lungs. It was concluded that the route of migration of schistosomula from lungs to liver was entirely intravascular, with potentially several passages round the pulmonary-systemic circulation, before chance entry into arteries leading to the hepatic portal system. The proportion of schistosomula exiting from the pulmonary-systemic circuit was estimated as 0·14/day. A computer simulation produced values of 11 h and 5 h for the duration of migration through pulmonary and systemic capillary beds. The time for 1 complete circuit would thus be 16 h and the proportion of schistosomula exiting per circuit would be 0·095. This approximates to the proportion of cardiac output going to splanchnic organs in the resting rat (0·128). The narrowness of hepatic sinusoids may be one factor contributing to the sequestration of schistosomula in the hepatic portal system.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 2 , April 1980 , pp. 267 - 288
Copyright
Copyright © Cambridge University Press 1980

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References

Altman, P. L. & Ditmer, D. S. (1971). Respiration and Circulation. Bethesda, Maryland: Federation of American Societies for Experimental Biology.Google Scholar
Blahitka, J. & Rakusan, K. (1977). Blood flow in rats during hemorrhagic shock: differences between surviving and dying animals. Circulatory Shock 4, 79–93.Google ScholarPubMed
Bruce, J. I., Pezzlo, F., Yajima, Y. & McCarthy, J. E. (1974). Schistosoma mansoni: pulmonary phase of schistosomule migration studied by electron microscopy. Experimental Parasitology 35, 150–60.CrossRefGoogle ScholarPubMed
Clegg, J. A. & Smithers, S. R. (1968). Death of schistosome cercariae during penetration of the skin. II. Penetration of mammalian skin by Schistosoma mansoni. Parasitology 58, 111–28.CrossRefGoogle ScholarPubMed
Coelho, P. M. Z., Pellegrino, J., Pereira, L. H. & Mello, R. T. (1976). Migration of schistosomula (Schistosoma mansoni) collected from hamsters and inoculated intraperitoneally in mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 161.CrossRefGoogle ScholarPubMed
Faust, E. C., Jones, C. A. & Hofeman, W. A. (1934). Studies on schistosomiasis mansoni in Puerto Rico III biological studies. 2. The mammalian phase of the life cycle. Puerto Rico Journal of Public Health and Tropical Medicine 10, 133–96.Google Scholar
Faust, E. C. & Meleney, H. E. (1924). Studies on schistosomiasis japonica American Journal of Hygiene, Monographic Series 3, 1–339.Google Scholar
Kruger, S. P., Heitman, L. P., Van Wyk, J. A. & McCully, R. M. (1969). The route of migration of Schistosoma matthei from lungs to liver in sheep. Journal of the South African Veterinary Medical Association 40, 39–43.Google Scholar
Lewert, R. M. & Mandlowitz, S. (1963). Innate immunity to Schistosoma mansoni relative to the state of connective tissue. Annals of the New York Academy of Sciences 113, 54–62.CrossRefGoogle Scholar
Madden, R. E., Paparo, M. S. & Schwartz, M. (1968). Limiting vascular diameters in various organs. Archives of Surgery 96, 130–7.CrossRefGoogle ScholarPubMed
Miller, P. (1976). Migration of the human blood fluke Schistosoma mansoni and its subsequent development in the mammalian host. D.Phil, thesis, University of York.Google Scholar
Miller, P. & Wilson, R. A. (1978). Migration of the schistosomula of Schistosoma mansoni from skin to lungs. Parasitology 77, 281–302.CrossRefGoogle ScholarPubMed
Pereira, L. H., Coelho, P. M. Z., Fonseca, J. J. A., Bredl, A. & Pellegrino, J. (1972). Migration of Schistosoma mansoni larvae in the albino mouse. Revista do Instituto de Medicina Tropical de Sao Paulo 14, 306–9.Google ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. (1959). Chronological analysis, pattern, and rate of migration of cercariae of Schistosoma mansoni in body, ear and tail skin of mice. Annals of Tropical Medicine and Parasitology 53, 400–13.CrossRefGoogle ScholarPubMed
Wheater, P. R. & Wilson, R. A. (1979). Schistosoma mansoni: a histological study of migration in the laboratory mouse. Parasitology 75, 49–62.CrossRefGoogle Scholar
Wilks, N. E. (1967). Lungs to liver migration of schistosomes in the laboratory mouse. American Journal of Tropical Medicine and Hygiene 16, 599–605.CrossRefGoogle ScholarPubMed
Wilson, R. A., Draskau, T., Miller, P.Lawson, J. R. (1978). Schistosoma mansoni: the activity and development of the schistosomulum during migration from the skin to the hepatic portal system. Parasitology 77, 57–73.CrossRefGoogle Scholar
Yolles, T. K., Moore, D. V. & Meleney, H. E. (1949). Post-cercarial development of Schistosoma mansoni in the rabbit and hamster after intra-peritoneal and percutaneous infection. Journal of Parasitology 35, 276–94.CrossRefGoogle Scholar