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A method for determining the pH of small quantities of fluid

Published online by Cambridge University Press:  06 April 2009

Ann Bishop ,
E. F. Hartree  and
Elspeth W. McConnachie
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Extract

The micro-glass electrode used in measuring the pH of blood from the stomachs of mosquitoes was a modification of that described by Caldwell (1954). The electrode was made of capillary tubing, drawn from Corning 015 glass, the outer diameter being 0·5–0·75 mm. and the length about 6 cm. As the electrode was used with a Pye Universal pH meter, and not with the electrometer impedance converter circuit used by Caldwell, it was important that the wall of the capillary tube should be very thin, in order that the resistance of the electrode should not exceed 500 MΩ, the maximum electrode resistance permissible with the Pye Universal pH meter.

Type
Research Article
Information
Parasitology , Volume 46 , Issue 1-2 , May 1956 , pp. 216 - 219
Copyright
Copyright © Cambridge University Press 1956

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References

REFERENCES

Caldwell, P. C. (1954). An investigation of the intracellular pH of crab muscle fibres by means of micro-glass and micro-tungsten electrodes. J. Physiol. 126, 169.CrossRefGoogle ScholarPubMed
Chinoy, J. J. (1947). A micro-glass electrode for pH determination. Curr. Sci. 16, 25.Google ScholarPubMed
Cole, S. W. (1933). Practical Physiological Chemistry. Cambridge: W. Heffer & Sons, Ltd.Google Scholar
Dietz, V. H. (1948). A simple micro-beaker for use with the Beckman pH meter (model G). Science, 108, 338.CrossRefGoogle Scholar
Hartre, E. F. (1952). A micro glass electrode. Biochem. J. 52, 619.CrossRefGoogle Scholar
Païc, M. (1938). Sur un nouveau type d'électrode de verre, pouvant servir de microélectrode. J. Chim. Phys. 35, 327.CrossRefGoogle Scholar