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Merozoite proteins from Babesia sp. BQ1 (Lintan) as potential antigens for serodiagnosis by ELISA

Published online by Cambridge University Press:  29 January 2010

G. Q. GUAN ,
A. CHAUVIN ,
H. ROGNIAUX ,
J. X. LUO ,
H. YIN  and
E. MOREAU
G. Q. GUAN
Affiliation:
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China Ecole Nationale Vétérinaire de Nantes, UMR 1300 BioEpAR, Atlanpole – La Chantrerie, BP 40706, F-44307 Nantes cedex 03, France
A. CHAUVIN
Affiliation:
Ecole Nationale Vétérinaire de Nantes, UMR 1300 BioEpAR, Atlanpole – La Chantrerie, BP 40706, F-44307 Nantes cedex 03, France
H. ROGNIAUX
Affiliation:
UR1268 Biopolymères Interactions Assemblages, Plate-Forme BIBS, INRA, F-44300 Nantes, France
J. X. LUO
Affiliation:
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China
H. YIN
Affiliation:
Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China
E. MOREAU*
Affiliation:
Ecole Nationale Vétérinaire de Nantes, UMR 1300 BioEpAR, Atlanpole – La Chantrerie, BP 40706, F-44307 Nantes cedex 03, France
*
*Corresponding author: Ecole Nationale Vétérinaire de Nantes, UMR 1300 BioEpAR, ENVN, Atlanpole – La Chantrerie, BP 40706, F-44307 Nantes cedex 03, France. Tel: 33 02 40 68 77 57. Fax: 33 02 40 68 77 51. E-mail: [email protected].
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Summary

Babesia sp. BQ1 (Lintan) is a Babesia isolated from sheep infested with Haemaphysalis qinghaiensis in China, and is closely related to B. motasi based on the 18S rRNA gene sequence. In the present study, an ELISA was developed with merozoite antigens of Babesia sp. BQ1 (Lintan) (BQMA) purified from in vitro culture. When the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95·5%. Except for Babesia sp. Tianzhu, there was no cross-reaction between BQMA and positive sera from Babesia sp. BQ1 (Ningxian)-, Babesia sp. Hebei-, Babesia sp. Xinjiang-, Theileria luwenshuni-, T. uilenbergi-, or Anaplasma ovis-infected sheep, which are the dominant haemoparasites of small ruminants in China. Specific antibodies against Babesia sp. BQ1 (Lintan) were produced 1 or 2 weeks post-infection and a high level of antibodies persisted for more than 8 months in experimentally infected sheep. This ELISA was tested on 974 sera collected from field-grazing sheep in 3 counties of Gansu province, northwestern China to evaluate the seroprevalence of Babesia sp. BQ1 (Lintan) infection and the average positive rate was 66·84%. The feasibility of increasing the specificity of this BQMA-based ELISA, by using some BQMA antigens for serodiagnosis is discussed.

Keywords

Babesia sp. BQ1 (Lintan)antigenELISAmerozoitecross-reactionantibody kineticsprevalence
Type
Research Article
Information
Parasitology , Volume 137 , Issue 6 , May 2010 , pp. 927 - 938
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Alani, A. J. and Herbert, I. V. (1980). The morphometrics of Babesia motasi (Wales) and its transmission by Haemaphysalis punctata (Canestrni and Fanzago, 1877) to sheep. Veterinary Parasitology 30, 87–95.CrossRefGoogle Scholar
Bai, Q., Liu, G. Y., Liu, D. K., Ren, J. X. and Li, X. (2002). Isolation and preliminary characterization of a large Babesia sp. from sheep and goats in the eastern part of Gansu Province, China. Parasitology Research 88, S16–S21.CrossRefGoogle ScholarPubMed
Birkenheuer, A. J., Levy, M. G., Stebbins, M., Poore, M. and Breitschwerdt, E. (2003). Serosurvey of anti-Babesia antibodies in stray dogs and American pit bull terriers and American Staffordshire terriers from North Carolina. Journal of the American Animal Hospital Association 39, 551–557.CrossRefGoogle Scholar
Brandao, L. P., Hagiwara, M. K. and Myiashiro, S. I. (2003). Humoral immunity and reinfection resistance in dogs experimentally inoculated with Babesia canis and either treated or untreated with imidocarb dipropionate. Veterinary Parasitology 114, 253–265.CrossRefGoogle ScholarPubMed
Brüning, R. (1996). Equine piroplasmosis: an update on diagnosis, treatment, and prevention. British Veterinary Journal 152, 139–151.CrossRefGoogle ScholarPubMed
Chauvin, A., Bouvet, G. and Boulard, C. (1995 a). Humoral and cellular immune responses to Fasciola hepatica experimental primary and secondary infection in sheep. International Journal for Parasitology 25, 1227–1241.CrossRefGoogle ScholarPubMed
Chauvin, A., L'hostis, M., Valentin, A., Precigout, E., Cesbron-Zeggane, N. and Gorenflot, A. (1995 b). Babesia divergens: an ELISA with soluble parasite antigen for monitoring the epidemiology of bovine babesiosis. Parasite 2, 257–262.CrossRefGoogle ScholarPubMed
Chen, D. M. (1982). Investigations on ovine Piroplasmosis. Chinese Journal for Veterinary Science and Technology 3, 31–32.Google Scholar
Christensson, D. and Thunegard, E. (1981). Babesia motasi in sheep on the island of Gotland in Sweden. Veterinary Parasitology 9, 99–106.CrossRefGoogle ScholarPubMed
Duzgun, A., Wright, I. G., Waltisbuhl, D. J., Gale, K. R., Goodger, B. V., Dargie, J. D., Alabay, M. and Cerci, H. (1991). An ELISA for the diagnosis of Babesia ovis infection utilizing a synthetic, Babesia bovis-derived antigen. Veterinary Parasitology 39, 225–231.CrossRefGoogle ScholarPubMed
Ferrer, D., Castellà, J., Gutiérrez, J. F., Lavín, S. and Marco, I. (1998). Seroprevalence of Babesia ovis in Spanish ibex (Capra pyrenaica) in Catalonia, northeastern Spain. Veterinary Parasitology 75, 93–98.CrossRefGoogle ScholarPubMed
Friedhoff, K. T. (1988). Transmission of Babesia. In Babesiosis of Domestic Animals and Man (ed. Ristic, M.), pp. 23–52. Boca Raton, Florida, USA.Google Scholar
Friedhoff, K. T. (1997). Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp. Parasitologia 39, 99–109.Google ScholarPubMed
Goff, W. L., McElwain, T. F., Suarez, C. E., Johnson, W. C., Brown, W. C., Norimine, J. and Knowles, D. P. (2003). Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle. Clinical and Diagnostic Laboratory Immunology 10, 38–43.Google ScholarPubMed
Guan, G. Q., Chauvin, A., Luo, J. X., Inoue, N., Moreau, E., Liu, Z. J., Gao, J. L., Oriel, M. M. THEKISOE, Ma, M. L., Liu, A. H., Dang, Z. S., Liu, J. L., Ren, Q. Y., Jin, Y. R., Sugimoto, C. and Yin, H. (2008). The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Experimental Parasitology 120, 39–44.CrossRefGoogle ScholarPubMed
Guan, G. Q., Ma, M. L., Moreau, E., Liu, J. L., Lu, B. Y., Bai, Q., Luo, J. X., Jorgensen, W., Chauvin, A. and Yin, H. (2009). A new ovine Babesia species transmitted by Hyalomma anatolicum anatolicum. Experimental Parasitology 122, 261–267.CrossRefGoogle ScholarPubMed
Guan, G. Q., Yin, H., Luo, J. X., Lu, W. S., Zhang, Q. C., Ma, M. L., Yuan, G. L., Lu, B. Y., Wang, Y. J. and Muhe, T. E. (2001). Isolation of a large ovine Babesia sp. in Xinjiang, China. Chinese Journal of Veterinary Science and Technology 31, 35–36.Google Scholar
Guan, G. Q., Yin, H., Luo, J. X., Lu, W. S., Zhang, Q. C., Gao, Y. L. and Lu, B. Y. (2002). Transmission of Babesia sp. to sheep with field-collected Haemaphysalis qinghaiensis. Parasitology Research 88, S22–S24.CrossRefGoogle ScholarPubMed
Habela, M., Reina, D., Nieto, C. and Navarrete, I. (1990). Antibody response and duration of latent infection in sheep following experimental infection with Babesia ovis. Veterinary Parasitology 35, 1–10.CrossRefGoogle ScholarPubMed
Hashemi-Fesharki, R. and Uilenberg, G. (1981). Babesia crassa n.sp (Sporozoa, Babesiidae) of domestic sheep in Iran. Veterinary Quarterly 2, 3–14.Google Scholar
He, L., Liu, Q., Quan, M., Zhou, D. N., Zhou, Y. Q., Zhao, J. L. (2009). Molecular cloning and phylogenetic analysis of Babesia orientalis heat shock protein 70. Veterinary Parasitology 162, 183–191.CrossRefGoogle ScholarPubMed
Ikadai, H., Osorio, C. R., Xuan, X. N., Igarashi, I., Kanemaru, T., Nagasawa, H., Fujisaki, K., Suzuki, N. and Mikami, T. (2000). Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoites rhoptry protein. International Journal for Parasitology 30, 633–635.CrossRefGoogle ScholarPubMed
Kjemtrup, A. M., Thomford, J. W., Gardner, I. A., Conrad, P. A., Jessup, D. A. and Boyce, W. M. (1995). Seroprevalence of two Babesia spp. isolates in selected bighorn sheep (Ovis canadensis) and mule deer (Odocoileus hemionus) populations in California. Journal of Wildlife Diseases 31, 467–471.CrossRefGoogle ScholarPubMed
Levine, N. D. (1985). Veterinary Protozoology, 1st Edn. Iowa State University Press, Ames, Iowa, USA.Google Scholar
Lewis, D., Holman, M. R., Purnell, R. E. and Young, E. R. (1981). Investigations on Babesia motasi isolated from Wales. Research in Veterinary Science 31, 239–243.CrossRefGoogle ScholarPubMed
Li, Y. Q., Luo, J. X., Gao, J. L., Guan, G. Q., Ma, M. L., Liu, Z. J., Liu, J. L., Liu, A. H., Ren, Q. Y., Dang, Z. S., Lu, B. Y., Liu, G. Y., Bai, Q. and Yin, H. (2009). Construction of cDNA expression library of Theileria luwenshuni and immunoscreening of Antigenic Genes. Scientia Agricultura Sinica 42, 331–339.Google Scholar
Liu, A. H., Yin, H., Guan, G. Q., Schnittger, L., Liu, Z. J., Ma, M. L., Dang, Z. S., Liu, J. L., Ren, Q. Y., Bai, Q., Ahmed, J. S. and Luo, J. X. (2007). At least two genetically distinct large Babesia species infective to sheep and goats in China. Veterinary Parasitology 147, 246–251.CrossRefGoogle ScholarPubMed
Liu, Z. J., Dang, Z. S., Luo, J. X., Yin, H., Ahmed, J. S. and Seitzer, U. (2008). Small-scale expressed sequence tag analysis of Theileria uilenbergi: Identification of a gene family encoding potential antigenic proteins. Annals of the New York Academy of Sciences 1149, 214–217.CrossRefGoogle ScholarPubMed
Malandrin, L., L'Hostis, M. and Chauvin, A. (2004). Isolation of Babesia divergens from carrier cattle blood using in vitro culture. Veterinary Research 35, 131–139.CrossRefGoogle ScholarPubMed
Miranda, J., Bakheit, M. A., Liu, Z., Yin, H., Mu, Y., Guo, S., Beyer, D., Oliva, A., Ahmed, J. S. and Seitzer, U. (2006). Development of a recombinant indirect ELISA for the diagnosis of Theileria sp. (China) infection in small ruminants. Parasitology Research 98, 561–567.CrossRefGoogle ScholarPubMed
Molloy, J. B., Bowles, P. M., Jeston, P. J., Bruyeres, A. G., Bowden, J. M., Bock, R. E., Jorgensen, W. K., Blight, G. W. and Dalgliesh, R. J. (1998). Development of an enzyme-linked immunosorbent assay for detection of antibodies to Babesia bigemina in cattle. Parasitology Research 84, 651–656.CrossRefGoogle ScholarPubMed
Na, B. K., Park, J. W., Lee, H. W., Lin, K., Kim, S. H., Bae, Y. A., Sohn, W. M., Kim, T. S. and Kong, Y. (2007). Characterization of Plasmodium vivax heat shock protein 70 and evaluation of its value for serodiagnosis of tertian malaria. Clinical and Vaccine Immunology 14, 320–322.CrossRefGoogle ScholarPubMed
Niu, Q. L., Luo, J. X., Guan, G. Q., Liu, Z. J., Ma, M. L., Liu, A. H., Gao, J. L., Ren, Q. Y., Li, Y. Q., Qiu, J. X. and Yin, H. (2009). Differentiation of two ovine Babesia based on the ribosomal DNA internal transcribed spacer (ITS) sequences. Experimental Parasitology 121, 64–68.CrossRefGoogle ScholarPubMed
Papadopoulos, B., Perié, N. M. and Uilenberg, G. (1996). Piroplasms of domestic animals in the Macedonia region of Greece: 1. Serological cross-reactions. Veterinary Parasitology 63, 41–56.CrossRefGoogle ScholarPubMed
Schnittger, L., Yin, H., Gubbels, M. J., Beyer, D., Nieman, S., Jongejan, F. and Ahmed, J. S. (2003). Phylogeny of sheep and goat Theileria and Babesia parasites. Parasitology Research 91, 398–406.Google ScholarPubMed
Shin, Y. S., Lee, E. G., Shin, G. W., Kim, Y. R., Lee, E. Y., Kim, J. H., Jang, H., Gershwin, L. J., Kim, D. Y., Kim, Y. H., Kim, G. S., Suh, M. D. and Jung, T. S. (2004). Identification of antigenic proteins from Neospora caninum recognized by bovine immunoglobulins M, E, A and G using immunoproteomics. Proteomics 4, 3600–3609.CrossRefGoogle Scholar
Sun, C. Q., Liu, Z. J., Gao, J. L., Guan, G. Q., Ma, M. L., Luo, J. X. and Yin, H. (2008). Investigations into the natural infection rate of Haemaphysalis qinghaiensis with piroplasms using a nested PCR. Experimental and Applied Acarology 44, 107–114.CrossRefGoogle Scholar
Teng, K. F. (1991). Economic Insect Fauna of China, Fasc 39, Acari: Ixodiae. Science Press Beijing, China.Google Scholar
Tenter, A. M. and Friedhoff, K. T. (1986). Serodiagnosis of experimental and natural Babesia equi and B. caballi infections. Veterinary Parasitology 20, 49–61.CrossRefGoogle ScholarPubMed
Uilenberg, G. (2006). Babesia – A historical overview. Veterinary Parasitology 138, 3–10.CrossRefGoogle ScholarPubMed
Uilenberg, G., Rombach, M. C., Perie, N. M. and Zwart, D. (1980). Blood parasites of sheep in the Netherlands. II. Babesia motasi (Sporozoa, Babesiidae). Veterinary Quarterly 2, 3–14.CrossRefGoogle ScholarPubMed
Zhao, X. R., Li, C. R. and Min, Y. G. (1986). Investigations on ovine Babesiosis. Chinese Journal for Veterinary Science and Technology 1, 26–27.Google Scholar