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A major Litomosoides carinii microfilarial sheath glycoprotein (gp22): amino terminal sequence and immunological studies with corresponding synthetic peptides

Published online by Cambridge University Press:  06 April 2009

G. Bardehle ,
F. J. Conraths ,
F. Fahrenholz ,
M. Hintz ,
D. Linder ,
G. Schares ,
H.-H. Schott ,
B. Schützle ,
S. Stirm  and
W. Stüber
...Show all authors
G. Bardehle
Affiliation:
Biochemisches Institut am Klinikum
F. J. Conraths
Affiliation:
Institut für Parasitologie der Justus-Liebig-Universität, Gieβen
F. Fahrenholz
Affiliation:
Max-Planck-Institut für Biophysik, Frankfurt
M. Hintz
Affiliation:
Biochemisches Institut am Klinikum
D. Linder
Affiliation:
Biochemisches Institut am Klinikum
G. Schares
Affiliation:
Institut für Parasitologie der Justus-Liebig-Universität, Gieβen
H.-H. Schott
Affiliation:
Biochemisches Institut am Klinikum
B. Schützle
Affiliation:
Max-Planck-Institut für Biophysik, Frankfurt
S. Stirm
Affiliation:
Biochemisches Institut am Klinikum
W. Stüber
Affiliation:
Behringwerke AG, Marburg/L
H. Zahner
Affiliation:
Institut für Parasitologie der Justus-Liebig-Universität, Gieβen
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Extract

The major glycoprotein of the sheath of Litomosoides carinii microfilariae (gp22) was analysed for its amino acid and amino sugar composition. It is rich in proline, glutamine/glutamic acid and glycine and contains (N-acetyl)galactosamine. The N-terminal amino acid sequence was determined up to position 37. It consists of a group of 6 repeats of the pentapeptide sequence methionine-glycine-proline-glutamine-proline with two minor modifications in repeats 3–6, while the first two repeats follow the general pattern more loosely. Identical N-terminal amino acid sequences were found in at least two other sheath polypeptides (33 kDa, 39 kDa). Antisera prepared against 3 overlapping synthetic peptides corresponding to the amino terminus of gp22 recognized different epitopes. They all reacted with identical patterns of sheath polypeptides. The antisera failed to recognize antigens of 4th-stage larvae of L. carinii. In contrast, cross-reacting epitopes were detected in other parasite stages. Antisera reacted with material surrounding embryos and microfilariae in the uterus of females, and caused patchy fluorescence on the sheath of blood-derived and in vitro-released microfilariae.

Keywords

Litomosoides cariniimicrofilarial sheathsheath polypeptidesgp22N-terminal sequenceoligopeptides
Type
Research Article
Information
Parasitology , Volume 103 , Issue 3 , December 1991 , pp. 387 - 394
Copyright
Copyright © Cambridge University Press 1991

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