Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-26T01:07:51.626Z Has data issue: false hasContentIssue false
  • English
  • Français

Lethality of intestinal tissue extracts from Eimeria-infected chickens

Published online by Cambridge University Press:  06 April 2009

M. E. McKenzie ,
Joyce Johnson  and
P. L. Long
M. E. McKenzie
Affiliation:
Department of Poultry Science, University of Georgia, Athens, Georgia30602
Joyce Johnson
Affiliation:
Department of Poultry Science, University of Georgia, Athens, Georgia30602
P. L. Long
Affiliation:
Department of Poultry Science, University of Georgia, Athens, Georgia30602
Get access Rights & Permissions [Opens in a new window]

Extract

Evidence exists that lethal activity is associated with extracts from Eimeria tenella-infected caecal tissues. We examined tissues of the small intestine infected with other species of Eimeria for a similar lethal activity. Extracts were prepared from intestinal tissues of chickens infected with either E. acervulina, E. brunetti, E. maxima, E. mitis or E. necatrix. Appropriate control extracts from tissues of non-infected chickens were also prepared. Intravenous injection of the extracts into healthy chickens showed that lethality of infected tissue extracts was higher than control extracts and was independent of the site of infection within the intestine. Comparison of the lethality of caecal extracts from chickens infected with embryo-adapted or parent lines of E. tenella showed that lethality was independent of the recognized pathogenic characteristics of the lines. Also, it was demonstrated that lethal activity is associated with extracts of tissues containing massive numbers of arrested and non-arrested sporozoites and early trophozoites.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 3 , June 1985 , pp. 565 - 572
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bradford, M. J., Herrick, C. A. & Wolfe, H. R. (1947). Lethal effects of cecal contents from chickens infected with cecal coccidiosis and the inhibition of these effects with immune serum. Journal of Parasitology 33, 393–8.CrossRefGoogle Scholar
Finney, J. D. (1964). Statistical Method in Biological Assay, 2nd edn, pp. 525–31. London: Griffin.Google Scholar
Fulton, J. D. (1965). Toxic exudates in Toxoplasma gondii infections. Experimental Parasitology 17, 252–60.Google Scholar
Gore, T. C., Long, P. L., Kogut, M. & Johnson, J. (1983). Attenuation of Eimeria necatrix and E. tenella of U. S. origin by serial embryo passage. Avian Diseases 3, 569–76.CrossRefGoogle Scholar
Johnson, J. & Reid, W. M. (1970). Anticoccidial drugs: Lesion scoring techniques in battery and floor pen experiments with chickens. Experimental Parasitology 28, 30–6.CrossRefGoogle ScholarPubMed
Long, P. L. (1972). Eimeria tenella: reproduction, pathogenicity, and immunogenicity of a strain maintained in chick embryos by serial passage. Journal of Comparative Pathology 82, 429–37.CrossRefGoogle ScholarPubMed
Long, P. L. (1973). Pathology and pathogenicity of coccidial infections. In The Coccidia (ed. Hammond, D. M. and Long, P. L.), pp. 253–94. Baltimore: University Park Press.Google ScholarPubMed
Long, P. L., Johnson, J. & Wyatt, R. D. (1981). Pathological and clinical effects of Eimeria tenella in partially immune chickens. Journal of Comparative Pathology 91, 581–7.CrossRefGoogle ScholarPubMed
Senaud, J., Vendrely, R. & Tronche, P. (1968). Obtention, à partir d'un extrait de kystes de Sarcocystis tenella (Rail.), d'une faction protéique toxique pour le lapin. Comptes Rendus des Séances de la Société de Biologie et de ses Filiales 162, 1184–9.Google Scholar
Weinman, D. & Klatchko, H. J. (1950). Description of a toxin in toxoplasmosis. Yale Journal of Biology and Medicine 22, 323–6.Google Scholar
Weston, I. D. (1964). Micro-analysis in Medical Biochemistry, pp. 138–9. New York: Crane and Stratton Inc.Google Scholar
Witlock, D. R. (1980). Demonstration of thromboplastin-like activity in extracts from Eimeria tenella-infected and non-infected chicken ceca. Proceedings of the Helminthological Society of Washington 47, 122–8.Google Scholar
Witlock, D. R. & Brimfield, A. A. (1982). Further studies on toxic extracts from Eimeria tenella-infected chicken ceca. Proceedings of the Helminthological Society of Washington 49, 305–13.Google Scholar
Wolfe, H. R., Bradford, M. J. & Herrick, C. A. (1946). The thromboplastin effects of tissue extracts and their inhibitions by immune sera. Physiological Zoology 19, 148–54.CrossRefGoogle ScholarPubMed