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Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon

Published online by Cambridge University Press:  03 March 2004

I. L. MAURICIO ,
J. R. STOTHARD  and
M. A. MILES
I. L. MAURICIO
Affiliation:
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
J. R. STOTHARD
Affiliation:
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK. Current address: Department of Infectious Disease Epidemiology, Imperial College, Norfolk Place, Paddington, London W2 1PG, UK.
M. A. MILES
Affiliation:
Pathogen Molecular Biology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
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Abstract

Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.

Keywords

restriction fragment length polymorphismgenotypingKinetoplastidaLeishmania donovaniribosomal internal transcribed spacermini-exon
Type
Research Article
Information
Parasitology , Volume 128 , Issue 3 , March 2004 , pp. 263 - 267
Copyright
2004 Cambridge University Press

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