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The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation

Published online by Cambridge University Press:  06 April 2009

M. H. Wisher  and
M. E. Rose
M. H. Wisher
Affiliation:
Houghton Poultry Research Station, Huntingdon, Cambs PE17 2DA
M. E. Rose
Affiliation:
Houghton Poultry Research Station, Huntingdon, Cambs PE17 2DA
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Summary

A method is described for the reproducible purification of greater than 1·0 × 108 sporozoites of Eimeria spp. by centrifugation on metrizamide density-gradients. The mean contamination of the purified sporozoite fraction by sporocysts and sporocyst debris was less than 6% and the recovered sporozoites were fully viable, infective and ultrastructurally intact.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 3 , June 1984 , pp. 515 - 519
Copyright
Copyright © Cambridge University Press 1984

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