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Isospora hypoleucae sp. n. (Apicomplexa: Eimeriidae), a new coccidian parasite found in the Pied Flycatcher (Ficedula hypoleuca)

Published online by Cambridge University Press:  19 May 2009

O. V. DOLNIK ,
J. A. C. von RÖNN  and
S. BENSCH
O. V. DOLNIK*
Affiliation:
Institute for Polar Ecology, Wischhofstraße 1-3 Geb. 12, D-24148Kiel, Germany Department of Animal Ecology, Lund University, Ecology Building, Sölvegatan 37, 223 62Lund, Sweden
J. A. C. von RÖNN
Affiliation:
Max Planck Institute for Evolutionary Biology, August-Thienemann-Straße 2, 24306Plön, Germany Department of Animal Ecology, Lund University, Ecology Building, Sölvegatan 37, 223 62Lund, Sweden
S. BENSCH
Affiliation:
Department of Animal Ecology, Lund University, Ecology Building, Sölvegatan 37, 223 62Lund, Sweden
*
*Corresponding author: Institute for Polar Ecology, Wischhofstraße 1-3 Geb. 12, D-24148Kiel, Germany. Tel: ++49 0 4318860842. Fax: ++49 0 4316001210. E-mail: [email protected]
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Summary

A new Coccidia species is reported from the natural population of Pied Flycatcher (Ficedula hypoleuca) in northern Germany. Sporulated oocysts were found in faeces from 6 of 8 sampled adults. The spherical oocysts of the new Isospora species have a brownish, smooth, bi-layered wall. Average size of sporulated oocysts was 19·4×19·3 μm (17·5–22·8 μm×17·5–22·8 μm) with a shape index (length/width) of 1·0. The sporulated oocysts have no micropyle or residuum, but enclose several small polar granules that often cluster into 2–3 dumbbell-shaped formations. Sporocysts are slightly elongated, rounded at the end opposite the Stieda body, 15·3 μm×9·2 μm in size (13·8–16·1 μm× 8·5–10·3 μm), and have a shape index of 1·7 (1·6–1·8). The Stieda body has a prominent knob-like cap, whereas the substieda body is absent. Sporocysts contain a small compact sporocyst residuum and 4 sporozoites. COI haplotypes identical to those isolated from faecal oocysts were PCR amplified from the blood of 13-day-old nestlings, suggesting that the newly described species has extra-intestinal stages in blood. This represents the first description of a new avian Isospora species supported by molecular sequence data from the same oocysts that are described morphologically.

Keywords

Isospora hypoleucaeFicedula hypoleucamitochondrial COI gene
Type
Research Article
Information
Parasitology , Volume 136 , Issue 8 , July 2009 , pp. 841 - 845
Copyright
Copyright © Cambridge University Press 2009

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References

REFERENCES

Box, E. D. (1975). Exogenous stages of Isospora serini (Aragão) and Isospora canaria sp. n. in the canary (Serinus canarius Linnaeus). Journal of Protozoology 22, 165–169.CrossRefGoogle Scholar
Box, E. D. (1977). Life cycles of two Isospora species in the Canary, Serinus canarius Linnaeus. Journal of Protozoology 24, 56–67.CrossRefGoogle ScholarPubMed
Box, E. (1981). Isospora as an extraintestinal parasite of passerine birds. Journal of Protozoology 28, 244–246.CrossRefGoogle Scholar
Černá, Ž. (1973). Interesting coccidians from wild birds. Journal of Protozoology 20, 535–536.Google Scholar
Cringoli, G. and Quesada, A. (1990). Isospora couderti sp.n., Isospora aycardii sp.n. chez Cisticola jundicis (Cisticole des Jones) (Passeriformes: Sylviidae) et Isospora landauae sp.n. chez Muscicapa hypoleuca (Gobe-mouche) (Passenformes: Muscicapidae). Annales de Parasitologie Humaine et Comparée 65, 167–169.CrossRefGoogle Scholar
Dolnik, O. (2002 a). Some aspects of the biology and host-parasite interactions of Isospora spp. (Protozoa: Coccidiida) of passerine birds. Ph.D. dissertation, Carl von Ossietzky University, Oldenburg, Germany. Online: available at: http://docserver.bis.uni-oldenburg.de/publikationen/dissertation/2003/dolsom02/dolsom02.htmlGoogle Scholar
Dolnik, O. V. (2002 b). Isospora sylvianthina (Protozoa: Coccidiida), parasite of Blackcap, does not infect Reed Warbler. Zoosystematica Rossica 10, 240.CrossRefGoogle Scholar
Dolnik, O. (2006). The relative stability of chronic Isospora sylvianthina (Protozoa: Apicomplexa) infection in blackcaps (Sylvia atricapilla): evaluation of a simplified method of estimating isosporan infection intensity in passerine birds. Parasitology Research 100, 55–60.CrossRefGoogle ScholarPubMed
Dolnik, O., Palinauskas, V. and Bensch, S. (2009). Individual oocysts of Isospora (Apicomplexa: Coccidia) parasites from avian faeces: from photo to sequence. The Journal of Parasitology 95, 169–174. DOI: 10.1645/GE-1873.1CrossRefGoogle ScholarPubMed
Duszynski, D. W. and Wilber, P. G. (1997). A guideline for the preparation of species descriptions in the eimeriidae. Journal of Parasitology 83, 333–336.CrossRefGoogle ScholarPubMed
Duszynski, D. W. (1999). Critical comment: revisiting the code: clarifying name-bearing types for photomicrographs of protozoa. The Journal of Parasitology 85, 769–770.CrossRefGoogle Scholar
Hall, T. A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Levine, N. D. (1982 a). Isospora passeris n. sp. from the house sparrow Passer domesticus, I. lacazei, and related apicomplexan Protozoa. Transactions of the American Microscopical Society 101, 66–74.CrossRefGoogle Scholar
Levine, N. D. (1982 b). The genus Atoxoplasma (Protozoa: Apicomplexa). The Journal of Parasitology 68, 719–723.CrossRefGoogle ScholarPubMed
Long, P. L. (ed.) (1982). The Biology of the Coccidia. Edward Arnold, London, UK.Google Scholar
Lundberg, A. and Alatalo, R. V. (1992). The Pied Flycatcher. T & A Poyser, London, UK.Google Scholar
Pellerdy, L. P. (1974). Coccidia and Coccidiosis. Akademiai Kiado, Budapest, Hungary.Google Scholar
Sambrook, J., Fritsch, F. J. and Maniatis, T. (2002). Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold, Spring Harbor, New York, USA.Google Scholar
Schrenzel, M. D., Maalouf, G. A., Gaffney, P. M., Tokarz, D., Keener, L. L., McClure, D., Griffey, S., McAloose, D. and Rideout, B. A. (2005). Molecular characterization of isosporoid Coccidia (Isospora and Atoxoplasma spp.) in passerine birds. The Journal of Parasitology 91, 635–647.CrossRefGoogle ScholarPubMed
Schwalbach, G. (1959). Untersuchungen und Beobachtungen an Coccidien der Gattungen Eimeria, Isospora und Caryospora bei Vögeln mit einer Beschreibung von sechzehn neuen Arten. Archiv für Protistenkunde 104, 431–491.Google Scholar
Svobodová, M. (1994). Isospora, Caryospora and Eimeria (Apicomplexa: Eimeriidae) in Passeriform Birds from Czech Republic. Acta Protozoologica 33, 101–108.Google Scholar
Upton, S. J., Wilson, S. C., Norton, T. M. and Greiner, E. C. (2001). A new species of Isospora Schneider, 1881 (Apicomplexa: Eimeriidae) from the Bali (Rothschild's) mynah Leucopsar rothschildi (Passeriformes: Sturnidae), and comments concerning the genera Atoxoplasma Garnham, 1950 and Isospora. Systematic Parasitology 48, 47–53.CrossRefGoogle ScholarPubMed