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Isolation of second-generation schizonts of avian coccidia and their use in biochemical investigations

Published online by Cambridge University Press:  06 April 2009

S. James
S. James
Affiliation:
Imperial Chemical Industries Ltd, Pharmaceuticals Division, Alderley Park, Macclesfield, Cheshire SK10 4TG
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Summary

A technique is described for the isolation of a pure suspension of second-generation schizonts of Eimeria necatrix and E. tenella. Most of the schizonts appeared viable as judged by their ability to exclude Trypan blue and the use of this criterion is examined. They were able to metabolize radio-isotope labelled glucose and accumulate thiamine against a concentration gradient. A formula allowing detailed consideration of the latter process is introduced. Crude extracts of these schizonts exhibited serine hydroxymethyl transferase activity which is significantly different from that of the host tissue in terms of optimal pH (8·3 for the parasite and 7·8 for the avian intestinal cells) and Michaelis constant (approximately 2·6 × 10−3m and 0·6 × 10−3m respectively). Differences in response to potential inhibitors are also shown.

Type
Research Article
Information
Parasitology , Volume 80 , Issue 2 , April 1980 , pp. 301 - 312
Copyright
Copyright © Cambridge University Press 1980

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