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Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix

Published online by Cambridge University Press:  06 April 2009

M. A. Fernando  and
J. Pasternak
M. A. Fernando
Affiliation:
Department of Pathology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N2G 2W2
J. Pasternak
Affiliation:
Department of Biology, Univeristy of Waterloo, Waterloo, ontario, Canada, N2L 3G1
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Extract

After infection with Eimeria necatrix, chick intestinal cells undergo a profound increase in size. Herien, we describe methods for isolating and purifying infected cells containing second-generation schizonts at Various times post-infection. Our purification procoedures depended upon three fundamental properties of infected cells: namely (1) large size, (2) resistance to shearing and (3) decreased susceptibility to osmotic shock in hypotonic solutions. The availability of simple techniques for isolating infected whole cells should prove valuable in elucidating various intracellular aspects of infection during schizogony.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 1 , February 1977 , pp. 19 - 26
Copyright
Copyright © Cambridge University Press 1977

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References

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