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Isolation and characterization of polysomes from Trypanosoma brucei

Published online by Cambridge University Press:  06 April 2009

J. S. Cordingley  and
M. J. Turner
J. S. Cordingley
Affiliation:
Medical Research Council Biochemical Parasitology Unit, The Molteno Institute, Downing Street, Cambridge CB2 3EE
M. J. Turner
Affiliation:
Medical Research Council Biochemical Parasitology Unit, The Molteno Institute, Downing Street, Cambridge CB2 3EE
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Summary

The isolation of polysomes in bulk from bloodstream forms of Trypanosoma brucei is described. The polysomes are active in in vitro protein synthesis in the presence or absence of initiation inhibitors. Nascent variant surface antigen (VSA) has been detected on these polysomes using purified radio-iodinated antibody. EDTA-induced ribosomal sub-units and their large rRNA's are characterized. The 26S rRNA is nicked to produce 2 molecules which are both smaller than the 195 rRNA of the small sub-unit which is larger than that found in the majority of eukaryotic small sub-units.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 3 , December 1980 , pp. 537 - 552
Copyright
Copyright © Cambridge University Press 1980

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